Cy3B PEG8 methyltetrazine
Ordering information
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1167.39 |
| Solvent | DMSO |
Spectral properties
| Correction Factor (260 nm) | 0.048 |
| Correction Factor (280 nm) | 0.069 |
| Extinction coefficient (cm -1 M -1) | 1200001 |
| Excitation (nm) | 560 |
| Emission (nm) | 571 |
| Quantum yield | 0.581 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
See also: Cyanines
Molecular weight 1167.39 | Correction Factor (260 nm) 0.048 | Correction Factor (280 nm) 0.069 | Extinction coefficient (cm -1 M -1) 1200001 | Excitation (nm) 560 | Emission (nm) 571 | Quantum yield 0.581 |
Cy3® dye is one of the most common cyanine dyes used for labeling proteins, nucleic acids and other biological molecules. A variety of Cy3® dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. However, Cy3 suffers low fluorescence intensity. It is the least fluorescent dyes among all the Cy dyes. Cy3B is an improved version of Cy3 dyes with significantly increased fluorescence quantum yield and photostability. Cy3B PEG8 methyltetrazine can be used for conjugations with TCO-containing biological molecules under copper-free conditions. Compared to the other click reactions, the TCO-tetrazine reaction gives much higher yields under mild reaction conditions. Cy3B has poor water solubility. This Cy3B PEG8 methyltetrazine contains a PEG8 linker that significantly improves its water solubility. Cy3® is the trademark of GE Healthcare.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3B PEG8 methyltetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 85.661 µL | 428.306 µL | 856.612 µL | 4.283 mL | 8.566 mL |
| 5 mM | 17.132 µL | 85.661 µL | 171.322 µL | 856.612 µL | 1.713 mL |
| 10 mM | 8.566 µL | 42.831 µL | 85.661 µL | 428.306 µL | 856.612 µL |
Molarity calculator
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Spectrum
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Spectral properties
| Correction Factor (260 nm) | 0.048 |
| Correction Factor (280 nm) | 0.069 |
| Extinction coefficient (cm -1 M -1) | 1200001 |
| Excitation (nm) | 560 |
| Emission (nm) | 571 |
| Quantum yield | 0.581 |
Images
Figure 1. Chemical structure for Cy3B tetrazine.
References
View all 1 references: Citation Explorer
Rapid labeling of metabolically engineered cell-surface glycoconjugates with a carbamate-linked cyclopropene reporter.
Authors: Späte, Anne-Katrin and Bußkamp, Holger and Niederwieser, Andrea and Schart, Verena F and Marx, Andreas and Wittmann, Valentin
Journal: Bioconjugate chemistry (2014): 147-54
Authors: Späte, Anne-Katrin and Bußkamp, Holger and Niederwieser, Andrea and Schart, Verena F and Marx, Andreas and Wittmann, Valentin
Journal: Bioconjugate chemistry (2014): 147-54