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Cy3DIGE NHS ester

Cy3DIGE NHS ester, an equivalent of Cy3® NHS ester minimal dye, is one of the major colors used for labeling proteins subject to DIGE analysis. Cy3DIGE is commonly used together with C2DIGE and/or Cy5DIGE. Cy2DIGE, C3DIGE and Cy5DIGE are specially designed for precise comparison of protein expression in two or three samples of lysates. 
Cy3DIGE NHS ester, an equivalent of Cy3® NHS ester minimal dye, is one of the major colors used for labeling proteins subject to DIGE analysis. Cy3DIGE is commonly used together with C2DIGE and/or Cy5DIGE. Cy2DIGE, C3DIGE and Cy5DIGE are specially designed for precise comparison of protein expression in two or three samples of lysates. 
Ordering information
Price ()
Catalog Number25303
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight709.67
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)1500001
Excitation (nm)555
Emission (nm)569
Quantum yield0.151
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
709.67
Correction Factor (260 nm)
0.07
Correction Factor (280 nm)
0.073
Extinction coefficient (cm -1 M -1)
1500001
Excitation (nm)
555
Emission (nm)
569
Quantum yield
0.151
Cy3DIGE NHS ester, an equivalent of Cy3® NHS ester minimal dye, is one of the major colors used for labeling proteins subject to DIGE analysis. Cy3DIGE is commonly used together with C2DIGE and/or Cy5DIGE. Cy2DIGE, C3DIGE and Cy5DIGE are specially designed for precise comparison of protein expression in two or three samples of lysates. Perfect mobility matching and bright dye fluorescence allow the efficient detection and high resolution of minor proteins on 2D gel electrophoresis. Cy2DIGE, C3DIGE and Cy5DIGE are compatible to all imagers capable of detection of Cy2, Cy3, and Cy5 dyes. The gels labeled with Cy2DIGE, C3DIGE and Cy5DIGE are size- and charge-matched fluorescent dyes for detecting protein abundance differences in 2-D Fluorescence Difference Gel Electrophoresis (DIGE). Cy2DIGE, C3DIGE and Cy5DIGE detect up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel. The size- and charge-matched dyes enable co-migration of labeled samples within the gel. They are bright on proteins, allowing the minimal loss of signal during labeling, separation, and scanning. These dyes have minimal spectral overlap, minimizing the crosstalk that commonly contributes to high background. Their pH-insensitive fluorescence allows DIGEs run at a broad pH range. Cy2®, Cy3® and Cy5® are the trademarks of GE Healthcare.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy3DIGE NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM140.911 µL704.553 µL1.409 mL7.046 mL14.091 mL
5 mM28.182 µL140.911 µL281.821 µL1.409 mL2.818 mL
10 mM14.091 µL70.455 µL140.911 µL704.553 µL1.409 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.07
Correction Factor (280 nm)0.073
Extinction coefficient (cm -1 M -1)1500001
Excitation (nm)555
Emission (nm)569
Quantum yield0.151

Citations


View all 34 citations: Citation Explorer
LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis
Authors: Wu, Y., Lam, C. S., Tse, D. Y., To, C. H., Liu, Q., McFadden, S. A., Chun, R. K., Li, K. K., Bian, J., Lam, C.
Journal: Mol Med Rep (2018): 5571-5580
Proteomic Analysis of Bovine Pregnancy-specific Serum Proteins by 2D Fluorescence Difference Gel Electrophoresis
Authors: Lee, J. E., Lee, J. Y., Kim, H. R., Shin, H. Y., Lin, T., Jin, D. I.
Journal: Asian-Australas J Anim Sci (2015): 788-95
Comparative proteomic analysis of different Toxoplasma gondii genotypes by two-dimensional fluorescence difference gel electrophoresis combined with mass spectrometry
Authors: Zhou, D. H., Zhao, F. R., Nisbet, A. J., Xu, M. J., Song, H. Q., Lin, R. Q., Huang, S. Y., Zhu, X. Q.
Journal: Electrophoresis (2014): 533-45
Comparative proteomic analysis of Dan'er malts produced from distinct malting processes by two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE)
Authors: Li, X., Jin, Z., Gao, F., Lu, J., Cai, G., Dong, J., Yu, J., Yang, M.
Journal: J Agric Food Chem (2014): 9310-6
Proteomic analysis of the hippocampus in Alzheimer's disease model mice by using two-dimensional fluorescence difference in gel electrophoresis
Authors: Takano, M., Yamashita, T., Nagano, K., Otani, M., Maekura, K., Kamada, H., Tsunoda, S., Tsutsumi, Y., Tomiyama, T., Mori, H., Matsuura, K., Matsuyama, S.
Journal: Neurosci Lett (2013): 85-9
Application of fluorescence two-dimensional difference in-gel electrophoresis as a proteomic biomarker discovery tool in muscular dystrophy research
Authors: Carberry, S., Zweyer, M., Sw and ulla, D., Ohlendieck, K.
Journal: Biology (Basel) (2013): 1438-64
Two-dimensional fluorescence difference gel electrophoresis analysis of Listeria monocytogenes submitted to a redox shock
Authors: Ignatova, M., Guevel, B., Com, E., Haddad, N., Rossero, A., Bogard, P., Prevost, H., Guillou, S.
Journal: J Proteomics (2013): 13-27
Identification of new pathogenic candidates for diabetic macular edema using fluorescence-based difference gel electrophoresis analysis
Authors: Hern, undefined and ez, C., Garcia-Ramirez, M., Colome, N., Corraliza, L., Garcia-Pascual, L., Casado, J., Canals, F., Simo, R.
Journal: Diabetes Metab Res Rev (2013): 499-506
Discovery and target identification of an antiproliferative agent in live cells using fluorescence difference in two-dimensional gel electrophoresis
Authors: Park, J., Oh, S., Park, S. B.
Journal: Angew Chem Int Ed Engl (2012): 5447-51