Cy5 biotin conjugate

This bifunctional Cy5 biotin conjugate demonstrates excellent avidin binding and strong fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and emission. Cy5 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
3100	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cy5 biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	73.865 µL	369.325 µL	738.651 µL	3.693 mL	7.387 mL
5 mM	14.773 µL	73.865 µL	147.73 µL	738.651 µL	1.477 mL
10 mM	7.387 µL	36.933 µL	73.865 µL	369.325 µL	738.651 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1353.82 g/mol	=	mL	x	mM	=	mmol

Citations

View all 29 citations: Citation Explorer

Optimizing SPION Labeling for Single-Cell Magnetic Microscopy

Authors:

Pointner, Andre and Thalheim, Daniela and Belasi, Sarah and Heinen, Lukas and Bonato, Cristian and Luehmann, Tobias and Meijer, Jan and Tietze, Rainer and Alexiou, Christoph and Schneider-Stock, Regine and others,

Journal:

arXiv preprint arXiv:2505.20373 (2025)

Barcoded screening identifies nanocarriers for protein delivery to kidney

Authors:

Wang, Luyao and Zhou, Wen and Chen, Hang and Jia, Xiangqian and Zheng, Peiyuan and Jiang, Haolin and Wu, Mengling and Zhang, Yaning and Ding, Yanchao and Peng, Yexi and others,

Journal:

Nature Communications (2025): 899

Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence

Authors:

Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.

Journal:

Biochem Biophys Rep (2019): 127-131

Biotin-tagged fluorescent sensor to visualize 'mobile' Zn(2+) in cancer cells

Authors:

Fang, L., Trigiante, G., Kousseff, C. J., Crespo-Otero, R., Philpott, M. P., Watkinson, M.

Journal:

Chem Commun (Camb) (2018): 9619-9622

Synthesis of Two-Photon Active Tricomponent Fluorescent Probe for Distinguishment of Biotin Receptor Positive and Negative Cells and Imaging 3D-Spheroid

Authors:

Pal, K., Sharma, A., Koner, A. L.

Journal:

Org Lett (2018): 6425-6429

References

View all 81 references: Citation Explorer

A low-cost, high-performance system for fluorescence lateral flow assays

Authors:

Lee, Linda G and Nordman, Eric S and Johnson, Martin D and Oldham, Mark F

Journal:

Biosensors (2013): 360--373

Implementation of P22 viral capsids as nanoplatforms

Authors:

Kang, Sebyung and Uchida, Masaki and O'Neil, Alison and Li, Rui and Prevelige, Peter E and Douglas, Trevor

Journal:

Biomacromolecules (2010): 2804--2809

Evaluation of a new biotin-DOTA conjugate for pretargeted antibody-guided radioimmunotherapy (PAGRIT((R)))

Authors:

Urbano N, Papi S, Ginanneschi M, De Santis R, Pace S, Lindstedt R, Ferrari L, Choi S, Paganelli G, Chinol M.

Journal:

Eur J Nucl Med Mol Imaging. (2006)

A biotin-protein bond with stability in plasma

Authors:

Bogusiewicz A, Mock NI, Mock DM.

Journal:

Anal Biochem (2005): 98

Instability of the biotin-protein bond in human plasma

Authors:

Bogusiewicz A, Mock NI, Mock DM.

Journal:

Anal Biochem (2004): 156

Physical properties

Molecular weight	1353.82
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.02
Correction factor (280 nm)	0.03
Correction factor (482 nm)	0.009
Correction factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27 ¹ , 0.4 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Other instruments

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 29, 2025