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Cy5 biotin conjugate
This bifunctional Cy5 biotin conjugate demonstrates excellent avidin binding and strong fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and emission. Cy5 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Chemical structure for Cy5 biotin conjugate.
Chemical structure for Cy5 biotin conjugate.
CatalogSize
Price
Quantity
31005 mg
Price
 
Physical properties

Molecular weight1353.82
SolventWater
Spectral properties

Correction factor (260 nm)0.02
Correction factor (280 nm)0.03
Correction factor (482 nm)0.009
Correction factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)
250000
1
Excitation (nm)651
Emission (nm)670
Quantum yield
0.27
1
0.4
2
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Other instruments
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Page updated on October 13, 2025