Cy5 biotin conjugate
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1353.82 |
| Solvent | Water |
Spectral properties
| Correction Factor (260 nm) | 0.02 |
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (482 nm) | 0.009 |
| Correction Factor (565 nm) | 0.09 |
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 651 |
| Emission (nm) | 670 |
| Quantum yield | 0.271, 0.42 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
See also: Biotin and Streptavidin
Molecular weight 1353.82 | Correction Factor (260 nm) 0.02 | Correction Factor (280 nm) 0.03 | Correction Factor (482 nm) 0.009 | Correction Factor (565 nm) 0.09 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 651 | Emission (nm) 670 | Quantum yield 0.271, 0.42 |
This bifunctional Cy5 biotin conjugate demonstrates excellent avidin binding and strong fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and emission. Cy5 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.
Platform
Other instruments
Example protocol
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Cy5 biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 73.865 µL | 369.325 µL | 738.651 µL | 3.693 mL | 7.387 mL |
| 5 mM | 14.773 µL | 73.865 µL | 147.73 µL | 738.651 µL | 1.477 mL |
| 10 mM | 7.387 µL | 36.933 µL | 73.865 µL | 369.325 µL | 738.651 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Correction Factor (260 nm) | 0.02 |
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (482 nm) | 0.009 |
| Correction Factor (565 nm) | 0.09 |
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 651 |
| Emission (nm) | 670 |
| Quantum yield | 0.271, 0.42 |
Images
Figure 1. Chemical structure for Cy5 biotin conjugate.
Citations
View all 27 citations: Citation Explorer
Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence
Authors: Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.
Journal: Biochem Biophys Rep (2019): 127-131
Authors: Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.
Journal: Biochem Biophys Rep (2019): 127-131
Biotin-tagged fluorescent sensor to visualize 'mobile' Zn(2+) in cancer cells
Authors: Fang, L., Trigiante, G., Kousseff, C. J., Crespo-Otero, R., Philpott, M. P., Watkinson, M.
Journal: Chem Commun (Camb) (2018): 9619-9622
Authors: Fang, L., Trigiante, G., Kousseff, C. J., Crespo-Otero, R., Philpott, M. P., Watkinson, M.
Journal: Chem Commun (Camb) (2018): 9619-9622
Synthesis of Two-Photon Active Tricomponent Fluorescent Probe for Distinguishment of Biotin Receptor Positive and Negative Cells and Imaging 3D-Spheroid
Authors: Pal, K., Sharma, A., Koner, A. L.
Journal: Org Lett (2018): 6425-6429
Authors: Pal, K., Sharma, A., Koner, A. L.
Journal: Org Lett (2018): 6425-6429
A green fluorescent protein-based assay for high-throughput ligand-binding studies of a mycobacterial biotin protein ligase
Authors: Bond, T. E. H., Sorenson, A. E., Schaeffer, P. M.
Journal: Microbiol Res (2017): 35-39
Authors: Bond, T. E. H., Sorenson, A. E., Schaeffer, P. M.
Journal: Microbiol Res (2017): 35-39
Labeling of Cytoskeletal Proteins in Living Cells Using Biotin Ligase Carrying a Fluorescent Protein
Authors: Nishi, S., Yamamoto, C., Yoneda, S., Sueda, S.
Journal: Anal Sci (2017): 897-902
Authors: Nishi, S., Yamamoto, C., Yoneda, S., Sueda, S.
Journal: Anal Sci (2017): 897-902
Determination of Biotin in Infant, Pediatric, and Adult Nutritionals by High-Performance Liquid Chromatography and Fluorescence Detection: Single-Laboratory Validation, First Action 2016.11
Authors: Lin, Q., Ding, Y., Poh, F., Zhang, C., Pan, S. J., Schimpf, K. J.
Journal: J AOAC Int (2017): 145-151
Authors: Lin, Q., Ding, Y., Poh, F., Zhang, C., Pan, S. J., Schimpf, K. J.
Journal: J AOAC Int (2017): 145-151
Quantum dot-fluorescence in situ hybridisation for Ectromelia virus detection based on biotin-streptavidin interactions
Authors: Wang, T., Zheng, Z., Zhang, X. E., Wang, H.
Journal: Talanta (2016): 179-184
Authors: Wang, T., Zheng, Z., Zhang, X. E., Wang, H.
Journal: Talanta (2016): 179-184
Oligonucleotide-stabilized fluorescent silver nanoclusters for the specific and sensitive detection of biotin
Authors: Xiong, X., Tang, Y., Zhao, J., Zhao, S.
Journal: Analyst (2016): 1499-505
Authors: Xiong, X., Tang, Y., Zhao, J., Zhao, S.
Journal: Analyst (2016): 1499-505
Highly Selective Fluorescent Turn-On Probe for Protein Thiols in Biotin Receptor-Positive Cancer Cells
Authors: Sun, Q., Sun, D., Song, L., Chen, Z., Chen, Z., Zhang, W., Qian, J.
Journal: Anal Chem (2016): 3400-5
Authors: Sun, Q., Sun, D., Song, L., Chen, Z., Chen, Z., Zhang, W., Qian, J.
Journal: Anal Chem (2016): 3400-5
A biotin-conjugated glutathione-responsive FRET-based fluorescent probe with a ferrocenyl BODIPY as the dark quencher
Authors: Shi, W. J., Lo, P. C., Zhao, S., Wong, R. C., Wang, Q., Fong, W. P., Ng, D. K.
Journal: Dalton Trans (2016): 17798-17806
Authors: Shi, W. J., Lo, P. C., Zhao, S., Wong, R. C., Wang, Q., Fong, W. P., Ng, D. K.
Journal: Dalton Trans (2016): 17798-17806
References
View all 81 references: Citation Explorer
A low-cost, high-performance system for fluorescence lateral flow assays
Authors: Lee, Linda G and Nordman, Eric S and Johnson, Martin D and Oldham, Mark F
Journal: Biosensors (2013): 360--373
Authors: Lee, Linda G and Nordman, Eric S and Johnson, Martin D and Oldham, Mark F
Journal: Biosensors (2013): 360--373
Implementation of P22 viral capsids as nanoplatforms
Authors: Kang, Sebyung and Uchida, Masaki and O'Neil, Alison and Li, Rui and Prevelige, Peter E and Douglas, Trevor
Journal: Biomacromolecules (2010): 2804--2809
Authors: Kang, Sebyung and Uchida, Masaki and O'Neil, Alison and Li, Rui and Prevelige, Peter E and Douglas, Trevor
Journal: Biomacromolecules (2010): 2804--2809
Evaluation of a new biotin-DOTA conjugate for pretargeted antibody-guided radioimmunotherapy (PAGRIT((R)))
Authors: Urbano N, Papi S, Ginanneschi M, De Santis R, Pace S, Lindstedt R, Ferrari L, Choi S, Paganelli G, Chinol M.
Journal: Eur J Nucl Med Mol Imaging. (2006)
Authors: Urbano N, Papi S, Ginanneschi M, De Santis R, Pace S, Lindstedt R, Ferrari L, Choi S, Paganelli G, Chinol M.
Journal: Eur J Nucl Med Mol Imaging. (2006)
A biotin-protein bond with stability in plasma
Authors: Bogusiewicz A, Mock NI, Mock DM.
Journal: Anal Biochem (2005): 98
Authors: Bogusiewicz A, Mock NI, Mock DM.
Journal: Anal Biochem (2005): 98
Instability of the biotin-protein bond in human plasma
Authors: Bogusiewicz A, Mock NI, Mock DM.
Journal: Anal Biochem (2004): 156
Authors: Bogusiewicz A, Mock NI, Mock DM.
Journal: Anal Biochem (2004): 156
Design, synthesis, and evaluation of 5'-S-aminoethyl-N(6)- azidobenzyl-5'-thioadenosine biotin conjugate: a bifunctional photoaffinity probe for the es nucleoside transporter
Authors: Addo JK, Buolamwini JK.
Journal: Bioconjug Chem (2004): 536
Authors: Addo JK, Buolamwini JK.
Journal: Bioconjug Chem (2004): 536
Biotin is endogenously expressed in select regions of the rat central nervous system
Authors: McKay BE, Molineux ML, Turner RW.
Journal: J Comp Neurol (2004): 86
Authors: McKay BE, Molineux ML, Turner RW.
Journal: J Comp Neurol (2004): 86
Probing the interaction of the biotin-avidin complex with the relaxivity of biotinylated Gd-DTPA
Authors: Langereis S, Kooistra HA, van Genderen MH, Meijer EW.
Journal: Org Biomol Chem (2004): 1271
Authors: Langereis S, Kooistra HA, van Genderen MH, Meijer EW.
Journal: Org Biomol Chem (2004): 1271
Immunoassay of total prostate-specific antigen using europium(III) nanoparticle labels and streptavidin-biotin technology
Authors: Huhtinen P, Soukka T, Lovgren T, Harma H.
Journal: J Immunol Methods (2004): 111
Authors: Huhtinen P, Soukka T, Lovgren T, Harma H.
Journal: J Immunol Methods (2004): 111
Volume of RBCs, 24- and 48-hour posttransfusion survivals, and the lifespan of (51)Cr and biotin-X-N-hydroxysuccinimide (NHS)-labeled autologous baboon RBCs: effect of the anticoagulant and blood pH on (51)Cr and biotin-X-NHS elution in vivo
Authors: Valeri CR, Pivacek LE, Cassidy GP, Ragno G.
Journal: Transfusion (2002): 343
Authors: Valeri CR, Pivacek LE, Cassidy GP, Ragno G.
Journal: Transfusion (2002): 343