logo
AAT Bioquest

Cy5 biotin conjugate

Product Image
Product Image
Gallery Image 1
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight1353.82
SolventWater
Spectral properties
Correction Factor (260 nm)0.02
Correction Factor (280 nm)0.03
Correction Factor (482 nm)0.009
Correction Factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)651
Emission (nm)670
Quantum yield0.271, 0.42
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Related products
Cy5NS acid
Cy5NS succinimidyl ester
Cy5.5NS, acid
Cy5.5NS succinimidyl ester
Cy5 trans-cyclooctene [Cy5 TCO]
Cy5 tertrazine [Cy5 tertrazine]
Cy5 phosphoramidite
Cy5® goat anti-mouse IgG (H+L)
Cy5® goat anti-mouse IgG (H+L) *Cross Adsorbed*
Cy5® goat anti-rabbit IgG (H+L)
Cy5® goat anti-rabbit IgG (H+L) *Cross Adsorbed*
Cy5 tyramide
Cy5 tetrazine
Cy5DIGE NHS ester
Cy5.5 biotin conjugate
Cy5 aldehyde
Cy5 Dye qPCR Calibration Solution *10,000X*
Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
Cy5.5 tetrazine
Biotin *CAS 58-85-5*
Biotin, succinimidyl ester *CAS 35013-72-0*
Biotin ethylenediamine *CAS 1217450-40-2*
Biotin cadaverine
Biotin C2 maleimide
Biotin-4-fluorescein *CAS 1032732-74-3*
Biotin hydrazide *CAS 66640-86-6*
Biotin-X NTA [Biotin-X nitrilotriacetic acid, potassium salt] *CAS 856661-92-2*
Biotin-X, succinimidyl ester *CAS 72040-63-2*
Biotin PEG2 amine *CAS 138529-46-1*
Biotin PEG2 maleimide *CAS 305372-39-8*
Biotin PEG2 succinimidyl ester
Biotin-PEG3-azide *CAS 875770-34-6*
Biotin Azide
Biotin Alkyne *CAS 773888-45-2*
Biotin PEG4 succinimidyl ester
ReadiView™ biotin acid
ReadiView™ biotin amine
ReadiView™ biotin hydrazide
ReadiView™ biotin maleimide
ReadiView™ biotin succinimidyl ester
ReadiLink™ Protein Biotinylation Kit *Powered by ReadiView™ Biotin Visionization Technology*
Amplite® Colorimetric Biotin Quantitation Kit
Biotin-11-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 86303-25-5*
Biotin-16-dUTP *1 mM in Tris Buffer (pH 7.5)* *CAS 136632-31-0*
Biotin-20-dUTP *1 mM in Tris Buffer (pH 7.5)*
Cal-520®-Biotin Conjugate
FMOC-Lys(Biotin)-OH
Biotin-14-dCTP *1 mM in Tris Buffer (pH 7.5)*
Biotin PEG3 amine
Annexin V-Biotin conjugate
N6-Methyladenosine-Biotin conjugate
Biotin Styramide *Superior Replacement for Biotin Tyramide*
2',3'-cGAMP-Biotin conjugate
Biotin-PEG4-tyramide
Biotin-11-dATP *1 mM in Tris Buffer (pH 7.5)*
Biotin-11-dGTP *1 mM in Tris Buffer (pH 7.5)*
Biotin NTA
Biotin-X IDA
ReadiCleave™ SSL biotin NHS ester
Biotin-dT Phosphoramidite
ReadiLink™ Biotin Nick Translation dsDNA Labeling Kit
ReadiLink™ Biotin Oligo and ssDNA Labeling Kit
AF532 PEG4 biotin conjugate
Biotin PEG 4 Alkyne
Biotin C2 Azide
mFluor™ UV 375 Biotin Conjugate
mFluor™ UV 460 Biotin Conjugate
mFluor™ Violet 500 Biotin Conjugate
mFluor™ Violet 540 Biotin Conjugate
mFluor™ Red 780 Biotin Conjugate
Biotin Phosphoramidite
Bio-16-UTP [Biotin-16-UTP] *1 mM*
Biotin PEG4 amine
mFluor™ Violet 450-PEG4-Biotin Conjugate
FastClick™ Biotin Azide
FastClick™ Biotin Alkyne
ReadiLeave™ Reversible Biotin Succinimidyl Ester
ReadiLeave™ Reversible Biotin Maleimide
ReadiLeave™ Reversible Biotin Azide
ReadiLeave™ Reversible Biotin Alkyne
ReadiLeave™ Reversible Biotin Amine
Show More (71)

OverviewpdfSDSpdfProtocol


Molecular weight
1353.82
Correction Factor (260 nm)
0.02
Correction Factor (280 nm)
0.03
Correction Factor (482 nm)
0.009
Correction Factor (565 nm)
0.09
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
651
Emission (nm)
670
Quantum yield
0.271, 0.42
This bifunctional Cy5 biotin conjugate demonstrates excellent avidin binding and strong fluorescence. It has avidin-binding properties similar to native biotin in terms of high affinity, fast association, and non-cooperative binding to avidin and streptavidin tetramers. Cy5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein, e.g., poor solubility in aqueous media and pH dependent absorbance and emission. Cy5 Biotin is a water-soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Platform


Other instruments

Example protocol


Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cy5 biotin conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM73.865 µL369.325 µL738.651 µL3.693 mL7.387 mL
5 mM14.773 µL73.865 µL147.73 µL738.651 µL1.477 mL
10 mM7.387 µL36.933 µL73.865 µL369.325 µL738.651 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.02
Correction Factor (280 nm)0.03
Correction Factor (482 nm)0.009
Correction Factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)651
Emission (nm)670
Quantum yield0.271, 0.42

Images


Citations


View all 27 citations: Citation Explorer
Streptavidin cooperative allosterism upon binding biotin observed by differential changes in intrinsic fluorescence
Authors: Waner, M. J., Hiznay, J. M., Mustovich, A. T., Patton, W., Ponyik, C., Mascotti, D. P.
Journal: Biochem Biophys Rep (2019): 127-131
Biotin-tagged fluorescent sensor to visualize &apos;mobile&apos; Zn(2+) in cancer cells
Authors: Fang, L., Trigiante, G., Kousseff, C. J., Crespo-Otero, R., Philpott, M. P., Watkinson, M.
Journal: Chem Commun (Camb) (2018): 9619-9622
Synthesis of Two-Photon Active Tricomponent Fluorescent Probe for Distinguishment of Biotin Receptor Positive and Negative Cells and Imaging 3D-Spheroid
Authors: Pal, K., Sharma, A., Koner, A. L.
Journal: Org Lett (2018): 6425-6429
A green fluorescent protein-based assay for high-throughput ligand-binding studies of a mycobacterial biotin protein ligase
Authors: Bond, T. E. H., Sorenson, A. E., Schaeffer, P. M.
Journal: Microbiol Res (2017): 35-39
Labeling of Cytoskeletal Proteins in Living Cells Using Biotin Ligase Carrying a Fluorescent Protein
Authors: Nishi, S., Yamamoto, C., Yoneda, S., Sueda, S.
Journal: Anal Sci (2017): 897-902
Determination of Biotin in Infant, Pediatric, and Adult Nutritionals by High-Performance Liquid Chromatography and Fluorescence Detection: Single-Laboratory Validation, First Action 2016.11
Authors: Lin, Q., Ding, Y., Poh, F., Zhang, C., Pan, S. J., Schimpf, K. J.
Journal: J AOAC Int (2017): 145-151
Quantum dot-fluorescence in situ hybridisation for Ectromelia virus detection based on biotin-streptavidin interactions
Authors: Wang, T., Zheng, Z., Zhang, X. E., Wang, H.
Journal: Talanta (2016): 179-184
Oligonucleotide-stabilized fluorescent silver nanoclusters for the specific and sensitive detection of biotin
Authors: Xiong, X., Tang, Y., Zhao, J., Zhao, S.
Journal: Analyst (2016): 1499-505
Highly Selective Fluorescent Turn-On Probe for Protein Thiols in Biotin Receptor-Positive Cancer Cells
Authors: Sun, Q., Sun, D., Song, L., Chen, Z., Chen, Z., Zhang, W., Qian, J.
Journal: Anal Chem (2016): 3400-5
A biotin-conjugated glutathione-responsive FRET-based fluorescent probe with a ferrocenyl BODIPY as the dark quencher
Authors: Shi, W. J., Lo, P. C., Zhao, S., Wong, R. C., Wang, Q., Fong, W. P., Ng, D. K.
Journal: Dalton Trans (2016): 17798-17806

References


View all 81 references: Citation Explorer
A low-cost, high-performance system for fluorescence lateral flow assays
Authors: Lee, Linda G and Nordman, Eric S and Johnson, Martin D and Oldham, Mark F
Journal: Biosensors (2013): 360--373
Implementation of P22 viral capsids as nanoplatforms
Authors: Kang, Sebyung and Uchida, Masaki and O'Neil, Alison and Li, Rui and Prevelige, Peter E and Douglas, Trevor
Journal: Biomacromolecules (2010): 2804--2809
Evaluation of a new biotin-DOTA conjugate for pretargeted antibody-guided radioimmunotherapy (PAGRIT((R)))
Authors: Urbano N, Papi S, Ginanneschi M, De Santis R, Pace S, Lindstedt R, Ferrari L, Choi S, Paganelli G, Chinol M.
Journal: Eur J Nucl Med Mol Imaging. (2006)
A biotin-protein bond with stability in plasma
Authors: Bogusiewicz A, Mock NI, Mock DM.
Journal: Anal Biochem (2005): 98
Instability of the biotin-protein bond in human plasma
Authors: Bogusiewicz A, Mock NI, Mock DM.
Journal: Anal Biochem (2004): 156
Design, synthesis, and evaluation of 5'-S-aminoethyl-N(6)- azidobenzyl-5'-thioadenosine biotin conjugate: a bifunctional photoaffinity probe for the es nucleoside transporter
Authors: Addo JK, Buolamwini JK.
Journal: Bioconjug Chem (2004): 536
Biotin is endogenously expressed in select regions of the rat central nervous system
Authors: McKay BE, Molineux ML, Turner RW.
Journal: J Comp Neurol (2004): 86
Probing the interaction of the biotin-avidin complex with the relaxivity of biotinylated Gd-DTPA
Authors: Langereis S, Kooistra HA, van Genderen MH, Meijer EW.
Journal: Org Biomol Chem (2004): 1271
Immunoassay of total prostate-specific antigen using europium(III) nanoparticle labels and streptavidin-biotin technology
Authors: Huhtinen P, Soukka T, Lovgren T, Harma H.
Journal: J Immunol Methods (2004): 111
Volume of RBCs, 24- and 48-hour posttransfusion survivals, and the lifespan of (51)Cr and biotin-X-N-hydroxysuccinimide (NHS)-labeled autologous baboon RBCs: effect of the anticoagulant and blood pH on (51)Cr and biotin-X-NHS elution in vivo
Authors: Valeri CR, Pivacek LE, Cassidy GP, Ragno G.
Journal: Transfusion (2002): 343