Name: Cy5 tetrazine
Brand: AAT Bioquest
SKU: 902
Price: 232 USD
Availability: InStock

Cy5 tetrazine

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This inverse electron demand cycloaddition reaction has gained popularity due to the potential for extremely fast cycloaddition kinetics with TCO as the dienophile. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this poweful click reaction. Cy5 tetrazine has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling peptides, proteins and oligos etc.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
902	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cy5 tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	87.989 µL	439.947 µL	879.894 µL	4.399 mL	8.799 mL
5 mM	17.598 µL	87.989 µL	175.979 µL	879.894 µL	1.76 mL
10 mM	8.799 µL	43.995 µL	87.989 µL	439.947 µL	879.894 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1136.5 g/mol	=	mL	x	mM	=	mmol

Physical properties

Molecular weight	1136.50
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.02
Correction factor (280 nm)	0.03
Correction factor (482 nm)	0.009
Correction factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27 ¹ , 0.4 ²

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 2, 2025