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Cy5 tetrazine

Chemical structure for Cy5 tetrazine.
Chemical structure for Cy5 tetrazine.
Ordering information
Price ()
Catalog Number902
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1136.50
SolventWater
Spectral properties
Correction Factor (260 nm)0.02
Correction Factor (280 nm)0.03
Correction Factor (482 nm)0.009
Correction Factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)651
Emission (nm)670
Quantum yield0.271, 0.42
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
Direct upgrades
iFluor® 647 Tetrazine

OverviewpdfSDSpdfProtocol


Molecular weight
1136.50
Correction Factor (260 nm)
0.02
Correction Factor (280 nm)
0.03
Correction Factor (482 nm)
0.009
Correction Factor (565 nm)
0.09
Extinction coefficient (cm -1 M -1)
2500001
Excitation (nm)
651
Emission (nm)
670
Quantum yield
0.271, 0.42
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This inverse electron demand cycloaddition reaction has gained popularity due to the potential for extremely fast cycloaddition kinetics with TCO as the dienophile. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this poweful click reaction. Cy5 tetrazine has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling peptides, proteins and oligos etc.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cy5 tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM87.989 µL439.947 µL879.894 µL4.399 mL8.799 mL
5 mM17.598 µL87.989 µL175.979 µL879.894 µL1.76 mL
10 mM8.799 µL43.995 µL87.989 µL439.947 µL879.894 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (260 nm)0.02
Correction Factor (280 nm)0.03
Correction Factor (482 nm)0.009
Correction Factor (565 nm)0.09
Extinction coefficient (cm -1 M -1)2500001
Excitation (nm)651
Emission (nm)670
Quantum yield0.271, 0.42

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)
Cy5 tertrazine [Cy5 tertrazine]65167025000010.271, 0.420.020.030.0090.09
Cy5 phosphoramidite65167025000010.271, 0.420.020.030.0090.09
Cy5 tyramide65167025000010.271, 0.420.020.030.0090.09
Cy5 aldehyde65167025000010.271, 0.420.020.030.0090.09
Cy3 tetrazine55556915000010.1510.070.073--
DBCO-Cy565167025000010.271, 0.420.020.030.0090.09
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*499520730000.921----
Cy3B Tetrazine56057112000010.5810.0480.069--