logo
AAT Bioquest

Cy5 tetrazine

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This inverse electron demand cycloaddition reaction has gained popularity due to the potential for extremely fast cycloaddition kinetics with TCO as the dienophile. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this poweful click reaction. Cy5 tetrazine has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling peptides, proteins and oligos etc.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Cy5 tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM87.989 µL439.947 µL879.894 µL4.399 mL8.799 mL
5 mM17.598 µL87.989 µL175.979 µL879.894 µL1.76 mL
10 mM8.799 µL43.995 µL87.989 µL439.947 µL879.894 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy5 tertrazine [Cy5 tertrazine]65167025000010.271, 0.420.020.03
Cy5 phosphoramidite65167025000010.271, 0.420.020.03
Cy5 tyramide65167025000010.271, 0.420.020.03
Cy5 aldehyde65167025000010.271, 0.420.020.03
Cy3 tetrazine55556915000010.1510.070.073
DBCO-Cy565167025000010.271, 0.420.020.03
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*499520710000.9210.300.11
Cy3B Tetrazine56057112000010.5810.0480.069
Cy7 tetrazine7567792500000.30.050.036
XFD350 tetrazine34344119000-0.250.19
XFD405 Tetrazine40142135,000-0.230.70
XFD430 Tetrazine43254015,000--0.28
XFD514 Tetrazine51854380000-0.310.18
XFD546 Tetrazine5615721120000.7910.210.12
XFD532 Tetrazine534553810000.6110.240.09
XFD568 Tetrazine579603913000.6910.450.46
XFD594 Tetrazine590618900000.6610.430.56
Show More (8)
Page updated on October 11, 2024

Ordering information

Price
Unit size
Catalog Number902
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

1136.50

Solvent

Water

Spectral properties

Correction Factor (260 nm)

0.02

Correction Factor (280 nm)

0.03

Correction Factor (482 nm)

0.009

Correction Factor (565 nm)

0.09

Extinction coefficient (cm -1 M -1)

2500001

Excitation (nm)

651

Emission (nm)

670

Quantum yield

0.271, 0.42

Storage, safety and handling

Intended useResearch Use Only (RUO)

Storage

Freeze (< -15 °C); Minimize light exposure
Product Image
Product Image
Gallery Image 1

Direct upgrades