Cy5 trans-cyclooctene [Cy5 TCO]

Cy5 trans-cyclooctene

Cy5 TCO

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This inverse electron demand cycloaddition reaction has gained popularity due to the potential for extremely fast cycloaddition kinetics with TCO as the dienophile. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this poweful click reaction. Cy5-TCO has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. It is widely used for labeling peptides, proteins and oligos etc.

Catalog	Size	Price	Quantity
901	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy5 trans-cyclooctene [Cy5 TCO] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	86.09 µL	430.448 µL	860.896 µL	4.304 mL	8.609 mL
5 mM	17.218 µL	86.09 µL	172.179 µL	860.896 µL	1.722 mL
10 mM	8.609 µL	43.045 µL	86.09 µL	430.448 µL	860.896 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1161.58 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Targeting Nanoparticles to Lung Cancer-derived A549 Cells Based on Changes on Interstitial Stiffness in Biomimetic Models

Authors:

Kohon, Afia Ibnat and Man, Kun and Hessami, Ala and Mathis, Katelyn and Webb, Jade and Fang, Joanna and Radfar, Parsa and Yang, Yong and Meckes, Brian

Journal:

iScience (2024)

Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene--methyltetrazine reactions

Authors:

Muraki, Michiro and Hirota, Kiyonori

Journal:

BMC biotechnology (2017): 56

References

View all 49 references: Citation Explorer

Beyond click chemistry - supramolecular interactions of 1,2,3-triazoles

Authors:

Schulze B, Schubert US.

Journal:

Chem Soc Rev (2014): 2522

Calixarene-based chemosensors by means of click chemistry

Authors:

Song M, Sun Z, Han C, Tian D, Li H, Kim JS.

Journal:

Chem Asian J (2014): 2344

Use of click-chemistry in the development of peptidomimetic enzyme inhibitors

Authors:

Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.

Journal:

Curr Med Chem (2014): 1467

Applications of copper-catalyzed click chemistry in activity-based protein profiling

Authors:

Martell J, Weerapana E.

Journal:

Molecules (2014): 1378

'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends

Authors:

M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.

Journal:

Expert Opin Ther Pat (2014): 1287

Physical properties

Molecular weight	1161.58
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.02
Correction factor (280 nm)	0.03
Correction factor (482 nm)	0.009
Correction factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27 ¹ , 0.4 ²

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025