Cy5DIGE NHS ester
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 707.65 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.02 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (482 nm) | 0.009 |
Correction Factor (565 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 651 |
Emission (nm) | 670 |
Quantum yield | 0.271, 0.42 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Amine Reactive Dyes and Probes for Conjugation, Bioconjugation, Dye to Peptide Conjugation, Chemical Reagents, Classic Dyes, Cyanines, Dyes by Functional Group, Peptide and Oligonucleotide Labeling
Molecular weight 707.65 | Correction Factor (260 nm) 0.02 | Correction Factor (280 nm) 0.03 | Correction Factor (482 nm) 0.009 | Correction Factor (565 nm) 0.09 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 651 | Emission (nm) 670 | Quantum yield 0.271, 0.42 |
Cy5DIGE NHS ester, an equivalent of Cy5® NHS ester minimal dye, is one of the major colors used for labeling proteins subject to DIGE analysis. Cy5DIGE is commonly used together with C2DIGE and/or Cy3DIGE. Cy2DIGE, C3DIGE and Cy5DIGE are specially designed for precise comparison of protein expression in two or three samples of lysates. Perfect mobility matching and bright dye fluorescence allow the efficient detection and high resolution of minor proteins on 2D gel electrophoresis. Cy2DIGE, C3DIGE and Cy5DIGE are compatible to all imagers capable of detection of Cy2, Cy3, and Cy5 dyes. The gels labeled with Cy2DIGE, C3DIGE and Cy5DIGE are size- and charge-matched fluorescent dyes for detecting protein abundance differences in 2-D Fluorescence Difference Gel Electrophoresis (DIGE). Cy2DIGE, C3DIGE and Cy5DIGE detect up to three prelabeled protein samples and standards on the same 2-D electrophoresis gel. The size- and charge-matched dyes enable co-migration of labeled samples within the gel. They are bright on proteins, allowing the minimal loss of signal during labeling, separation, and scanning. These dyes have minimal spectral overlap, minimizing the crosstalk that commonly contributes to high background. Their pH-insensitive fluorescence allows DIGEs run at a broad pH range. Cy2®, Cy3® and Cy5® are the trademarks of GE Healthcare.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cy5DIGE NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 141.313 µL | 706.564 µL | 1.413 mL | 7.066 mL | 14.131 mL |
5 mM | 28.263 µL | 141.313 µL | 282.626 µL | 1.413 mL | 2.826 mL |
10 mM | 14.131 µL | 70.656 µL | 141.313 µL | 706.564 µL | 1.413 mL |
Molarity calculator
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Spectrum
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Spectral properties
Correction Factor (260 nm) | 0.02 |
Correction Factor (280 nm) | 0.03 |
Correction Factor (482 nm) | 0.009 |
Correction Factor (565 nm) | 0.09 |
Extinction coefficient (cm -1 M -1) | 2500001 |
Excitation (nm) | 651 |
Emission (nm) | 670 |
Quantum yield | 0.271, 0.42 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester* | 490 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 303 | 439 | 19000 | - | 0.25 | 0.19 |
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester* | 317 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester* | 375 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
QXY21 NHS ester [equivalent to QSY-21 NHS ester] | - | - | 890001 | - | - | 0.32 |
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester* | 520 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester* | 604 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester* | 681 | 704 | 184000 | 0.361 | 0.00 | 0.05 |
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester* | 696 | 719 | 192000 | 0.251 | 0.00 | 0.07 |
Show More (8) |
Images

Figure 1. Cy5DIGE NHS ester, an equivalent of Cy5® NHS ester minimal dye, is one of the major colors used for labeling proteins subject to DIGE analysis. Cy5DIGE is commonly used together with C2DIGE and/or Cy3DIGE. Cy2DIGE, C3DIGE and Cy5DIGE are specially designed for precise comparison of protein expression in two or three samples of lysates.
Citations
View all 34 citations: Citation Explorer
LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
Early quantitative profiling of differential retinal protein expression in lens-induced myopia in guinea pig using fluorescence difference two-dimensional gel electrophoresis
Authors: Wu, Y., Lam, C. S., Tse, D. Y., To, C. H., Liu, Q., McFadden, S. A., Chun, R. K., Li, K. K., Bian, J., Lam, C.
Journal: Mol Med Rep (2018): 5571-5580
Authors: Wu, Y., Lam, C. S., Tse, D. Y., To, C. H., Liu, Q., McFadden, S. A., Chun, R. K., Li, K. K., Bian, J., Lam, C.
Journal: Mol Med Rep (2018): 5571-5580
Proteomic Analysis of Bovine Pregnancy-specific Serum Proteins by 2D Fluorescence Difference Gel Electrophoresis
Authors: Lee, J. E., Lee, J. Y., Kim, H. R., Shin, H. Y., Lin, T., Jin, D. I.
Journal: Asian-Australas J Anim Sci (2015): 788-95
Authors: Lee, J. E., Lee, J. Y., Kim, H. R., Shin, H. Y., Lin, T., Jin, D. I.
Journal: Asian-Australas J Anim Sci (2015): 788-95
Comparative proteomic analysis of different Toxoplasma gondii genotypes by two-dimensional fluorescence difference gel electrophoresis combined with mass spectrometry
Authors: Zhou, D. H., Zhao, F. R., Nisbet, A. J., Xu, M. J., Song, H. Q., Lin, R. Q., Huang, S. Y., Zhu, X. Q.
Journal: Electrophoresis (2014): 533-45
Authors: Zhou, D. H., Zhao, F. R., Nisbet, A. J., Xu, M. J., Song, H. Q., Lin, R. Q., Huang, S. Y., Zhu, X. Q.
Journal: Electrophoresis (2014): 533-45
Comparative proteomic analysis of Dan'er malts produced from distinct malting processes by two-dimensional fluorescence difference in gel electrophoresis (2D-DIGE)
Authors: Li, X., Jin, Z., Gao, F., Lu, J., Cai, G., Dong, J., Yu, J., Yang, M.
Journal: J Agric Food Chem (2014): 9310-6
Authors: Li, X., Jin, Z., Gao, F., Lu, J., Cai, G., Dong, J., Yu, J., Yang, M.
Journal: J Agric Food Chem (2014): 9310-6
Proteomic analysis of the hippocampus in Alzheimer's disease model mice by using two-dimensional fluorescence difference in gel electrophoresis
Authors: Takano, M., Yamashita, T., Nagano, K., Otani, M., Maekura, K., Kamada, H., Tsunoda, S., Tsutsumi, Y., Tomiyama, T., Mori, H., Matsuura, K., Matsuyama, S.
Journal: Neurosci Lett (2013): 85-9
Authors: Takano, M., Yamashita, T., Nagano, K., Otani, M., Maekura, K., Kamada, H., Tsunoda, S., Tsutsumi, Y., Tomiyama, T., Mori, H., Matsuura, K., Matsuyama, S.
Journal: Neurosci Lett (2013): 85-9
Application of fluorescence two-dimensional difference in-gel electrophoresis as a proteomic biomarker discovery tool in muscular dystrophy research
Authors: Carberry, S., Zweyer, M., Sw and ulla, D., Ohlendieck, K.
Journal: Biology (Basel) (2013): 1438-64
Authors: Carberry, S., Zweyer, M., Sw and ulla, D., Ohlendieck, K.
Journal: Biology (Basel) (2013): 1438-64
Two-dimensional fluorescence difference gel electrophoresis analysis of Listeria monocytogenes submitted to a redox shock
Authors: Ignatova, M., Guevel, B., Com, E., Haddad, N., Rossero, A., Bogard, P., Prevost, H., Guillou, S.
Journal: J Proteomics (2013): 13-27
Authors: Ignatova, M., Guevel, B., Com, E., Haddad, N., Rossero, A., Bogard, P., Prevost, H., Guillou, S.
Journal: J Proteomics (2013): 13-27
Identification of new pathogenic candidates for diabetic macular edema using fluorescence-based difference gel electrophoresis analysis
Authors: Hern, undefined and ez, C., Garcia-Ramirez, M., Colome, N., Corraliza, L., Garcia-Pascual, L., Casado, J., Canals, F., Simo, R.
Journal: Diabetes Metab Res Rev (2013): 499-506
Authors: Hern, undefined and ez, C., Garcia-Ramirez, M., Colome, N., Corraliza, L., Garcia-Pascual, L., Casado, J., Canals, F., Simo, R.
Journal: Diabetes Metab Res Rev (2013): 499-506
Discovery and target identification of an antiproliferative agent in live cells using fluorescence difference in two-dimensional gel electrophoresis
Authors: Park, J., Oh, S., Park, S. B.
Journal: Angew Chem Int Ed Engl (2012): 5447-51
Authors: Park, J., Oh, S., Park, S. B.
Journal: Angew Chem Int Ed Engl (2012): 5447-51
Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)