AAT Bioquest

Cy7 tetrazine

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Physical properties
Molecular weight1162.54
Spectral properties
Correction Factor (260 nm)0.05
Correction Factor (280 nm)0.036
Correction Factor (482 nm)0.0005
Correction Factor (565 nm)0.0193
Correction Factor (650 nm)0.165
Extinction coefficient (cm -1 M -1)250000
Excitation (nm)756
Emission (nm)779
Quantum yield0.3
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (260 nm)
Correction Factor (280 nm)
Correction Factor (482 nm)
Correction Factor (565 nm)
Correction Factor (650 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
Cy7 tetrazine is an excellent building block that can be readily used to label TCO-modified biological molecules (e.g., peptides, proteins and oligos etc) for NIR fluorescence in vivo imaging and other fluorescence-based biological applications. The inverse electron demand Diels–Alder (IEDDA) between 1,2,4,5-tetrazine and strained alkene (such as trans-cyclooctene, TCO) is a well-established bioorthogonal reaction. It is generally considered to be the fastest click reaction. Since the first report on IEDDA reaction, several kinds of strained alkenes/alkynes and tetrazine analogs have been synthesized, and these functional group pairs have been applied to drug discovery, life science research and nanomaterials. Due to the extremely rapid reaction rate of IEDDA under mild conditions such as room temperature, neutral pH, and in aqueous media, this reaction has been a highly useful ligation approach for a variety of bioconjugations. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cy7 tetrazine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM86.019 µL430.093 µL860.185 µL4.301 mL8.602 mL
5 mM17.204 µL86.019 µL172.037 µL860.185 µL1.72 mL
10 mM8.602 µL43.009 µL86.019 µL430.093 µL860.185 µL

Molarity calculator

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Spectral properties

Correction Factor (260 nm)0.05
Correction Factor (280 nm)0.036
Correction Factor (482 nm)0.0005
Correction Factor (565 nm)0.0193
Correction Factor (650 nm)0.165
Extinction coefficient (cm -1 M -1)250000
Excitation (nm)756
Emission (nm)779
Quantum yield0.3

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
Cy7 tyramide7567792500000.30.050.036
Cy7 Styramide *Superior Replacement for Cy7 tyramide*7567792500000.30.050.036
Cy3 tetrazine55556915000010.1510.070.073
Cy5 tetrazine65167025000010.271, 0.420.020.03
XFD488 tetrazine *Same Structure to Alexa Fluor™ 488 tetrazine*499520710000.9210.300.11
Cy3B Tetrazine56057112000010.5810.0480.069



View all 3 references: Citation Explorer
Signal Amplification Pretargeted PET/Fluorescence Imaging Based on Human Serum Albumin-Encapsulated GdF3 Nanoparticles for Diagnosis of Ovarian Cancer.
Authors: Shi, Xudong and Gao, Kai and Zhang, Guoxin and Zhang, Wenlong and Yang, Xingjiu and Gao, Ran
Journal: ACS biomaterials science & engineering (2022): 4956-4964
Enabling In Vivo Photocatalytic Activation of Rapid Bioorthogonal Chemistry by Repurposing Silicon-Rhodamine Fluorophores as Cytocompatible Far-Red Photocatalysts.
Authors: Wang, Chuanqi and Zhang, He and Zhang, Tao and Zou, Xiaoyu and Wang, Hui and Rosenberger, Julia E and Vannam, Raghu and Trout, William S and Grimm, Jonathan B and Lavis, Luke D and Thorpe, Colin and Jia, Xinqiao and Li, Zibo and Fox, Joseph M
Journal: Journal of the American Chemical Society (2021): 10793-10803
NIR-Cyanine Dye Linker: a Promising Candidate for Isochronic Fluorescence Imaging in Molecular Cancer Diagnostics and Therapy Monitoring.
Authors: Komljenovic, Dorde and Wiessler, Manfred and Waldeck, Waldemar and Ehemann, Volker and Pipkorn, Ruediger and Schrenk, Hans-Hermann and Debus, Jürgen and Braun, Klaus
Journal: Theranostics (2016): 131-41