Name: Cyanine 3.5 monosuccinimidyl ester [equivalent to Cy3.5® NHS ester]
Brand: AAT Bioquest
SKU: 148
Price: 115 USD
Availability: InStock

Cyanine 3.5 monosuccinimidyl ester

equivalent to Cy3.5® NHS ester

A variety of cyanine 3.5 (Cy3.5®) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy3.5® dyes have enhanced fluorescence upon binding to proteins. Cy3.5® NHS ester readily reacts with amino groups. Cy3® NHS ester readily reacts with amino groups. AAT Bioquest offers Cy dye NHS esters in the form of triethylammonium salts that are more soluble in DMSO and DMF than the corresponding potassium salts that are offered by some other vendors. The Cy dye triethylammonium salts have the same reactivity and give the conjugates identical to the the Cy dye potassium salts. Cy3.5® is the trademark of GE Healthcare.

Catalog	Size	Price	Quantity
148	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cyanine 3.5 monosuccinimidyl ester [equivalent to Cy3.5® NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	77.42 µL	387.099 µL	774.198 µL	3.871 mL	7.742 mL
5 mM	15.484 µL	77.42 µL	154.84 µL	774.198 µL	1.548 mL
10 mM	7.742 µL	38.71 µL	77.42 µL	387.099 µL	774.198 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1291.66 g/mol	=	mL	x	mM	=	mmol

Citations

View all 13 citations: Citation Explorer

Single-Molecule Characterization of Cy3. 5-Cy5. 5 Dye Pair for FRET Studies of Nucleic Acids and Nucleosomes

Authors:

Ghoneim, Mohamed and Musselman, Catherine A

Journal:

Journal of Fluorescence (2022): 1--9

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization

Authors:

Wang, Xiaoyan and Zhang, Yu and Xue, Wei and Wang, Hong and Qiu, Xiaozhong and Liu, Zonghua

Journal:

Journal of Biomaterials Applications (2017): 923--932

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy

Authors:

Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao

Journal:

Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics

Authors:

Guo, Yuxin and Zhang, Yang and Ma, Jinyuan and Li, Qi and Li, Yang and Zhou, Xinyi and Zhao, Dan and Song, Hua and Chen, Qing and Zhu, Xuan

Journal:

Journal of Controlled Release (2017)

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions

Authors:

Wang, Xu and Shi, Changying and Zhang, Li and Bodman, Alexa and Guo, D and an , undefined and Wang, Lili and Hall, Walter A and Wilkens, Stephan and Luo, Juntao

Journal:

Biomaterials (2016)

References

View all 21 references: Citation Explorer

Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators

Authors:

Freeman LM, Li S, Dayani Y, Choi HS, Malmstadt N, Armani AM.

Journal:

Appl Phys Lett (2011): 143703

Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy

Authors:

Wiessler M, Hennrich U, Pipkorn R, Waldeck W, Cao L, Peter J, Ehemann V, Semmler W, Lammers T, Braun K.

Journal:

Theranostics (2011): 381

Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging

Authors:

Berezin MY, Guo K, Akers W, Livingston J, Solomon M, Lee H, Liang K, Agee A, Achilefu S.

Journal:

Biochemistry (2011): 2691

In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium

Authors:

Adler ED, Bystrup A, Briley-Saebo KC, Mani V, Young W, Giovanonne S, Altman P, Kattman SJ, Frank JA, Weinmann HJ, Keller GM, Fayad ZA.

Journal:

JACC Cardiovasc Imaging (2009): 1114

Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes

Authors:

Volke D, Hoffmann R.

Journal:

Electrophoresis (2008): 4516

Physical properties

Molecular weight	1291.66
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.08
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	150000
Excitation (nm)	579
Emission (nm)	591
Quantum yield	0.15

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 13, 2025