Cyanine 3 monosuccinimidyl ester [equivalent to Cy3® NHS ester]

Image Viewer
Select color channel(s)
HeLa cells were incubated with (Tubulin+) or without (Tubulin-) mouse anti-tubulin followed by AAT’s Cy3<sup>®</sup> goat anti-mouse IgG conjugate (Red, Left) or Jackson’s goat anti-mouse IgG conjugated with Cy3<sup>®</sup>  (Red, Right), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).
Roll over image to zoom in


Loading...
 
Unit Size: Cat No: Price (USD): Qty:
1 mg 141 $95


Export item/cart as Excel file

Send item/cart as email
EXPORT TO EXCEL X

Export:
EXPORT TO EMAIL X
Important: We request your email address to ensure that the recipient(s) knows you intended for them to see the email, and that it is not junk mail.
Export:
Your Name*:
Your Email*:
Recipient Email*:
Your Personal Message:
Additional Ordering Information
Telephone: 1-800-990-8053
Fax: 1-408-733-1304
Email: sales@aatbio.com
International: See distributors





Overview

Ex/Em (nm)555/565
MW829.03
SolventDMSO
Storage Freeze (<-15 °C)
Minimize light exposure
Category Classic Labeling Dyes
Cyanines
Related Peptide Labeling Reagents
Dye Labeling Reagents
Secondary Reagents
A variety of cyanine 3 (Cy3®) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy3® dyes have enhanced fluorescence upon binding to proteins. Cy3® NHS ester readily reacts with amino groups. AAT Bioquest offers Cy dye NHS esters in the form of triethylammonium salts that are more soluble in DMSO and DMF than the corresponding potassium salts that are offered by some other vendors. The Cy dye triethylammonium salts have the same reactivity and give the conjugates identical to the the Cy dye potassium salts. Cy3® is the trademark of GE Healthcare.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cyanine 3 monosuccinimidyl ester [equivalent to Cy3® NHS ester] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 






Spectrum Advanced Spectrum Viewer

Sorry, your browser does not support inline SVG. Relative Intensity (%) 100 80 60 40 20 0 Sorry, your browser does not support inline SVG.
Sorry, your browser does not support inline SVG. Sorry, your browser does not support inline SVG.
Move mouse over grid to display wavelength & intensity values.

300
400
500
600
700
800
900
Wavelength (nm)









References & Citations

Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division
Authors: Zelin Shan, Yuting Tu, Ying Yang, Ziheng Liu, Menglong Zeng, Huisha Xu, Jiafu Long, Mingjie Zhang, Yu Cai, Wenyu Wen
Journal: Nature Communications (2018): 737

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal: Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Journal: Journal of Controlled Release (2017)

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Xiaoyan Wang, Yu Zhang, Wei Xue, Hong Wang, Xiaozhong Qiu, Zonghua Liu
Journal: Journal of Biomaterials Applications (2017): 923--932

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Xu Wang, Changying Shi, Li Zhang, Alexa Bodman, Dandan Guo, Lili Wang, Walter A Hall, Stephan Wilkens, Juntao Luo
Journal: Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors: Xiongwei Deng, Zhaoxia Yin, Zhixiang Zhou, Yihui Wang, Fang Zhang, Qin Hu, Yishu Yang, Jianqing Lu, Yan Wu, Wang Sheng
Journal: ACS applied materials & interfaces (2016): 17068--17079

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors: John T Ngo, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera, Sakina F Palida, Carolyn R Bertozzi, Mark H Ellisman, Roger Y Tsien
Journal: Nat Chem Biol (2016): 459--465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors: Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Journal: Drug Design, Development and Therapy (2016): 3851

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors: Cuiping Liu, Aaron J Stonestrom, Thomas Christian, Jeongsik Yong, Ryuichi Takase, Ya-Ming Hou, Xiaolu Yang
Journal: Journal of Biological Chemistry (2016): 10426--10436

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography
Authors: Takeaki Nagamine, Kou Hayakawa, Kyoumi Nakazato, Masahiko Iha
Journal: Journal of Chromatography B (2015): 187--193


View More Citations