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Cyanine 5.5 amine [equivalent to Cy5.5® amine]
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A variety of cyanine 5.5 (Cy5.5®) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5.5® dyes are one type of the most common red fluorophores. Cy5.5® amine reacts with carbonyl groups. Cy5.5® is the trademark of GE Healthcare.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Cyanine 5.5 amine [equivalent to Cy5.5® amine] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 84.233 µL | 421.166 µL | 842.332 µL | 4.212 mL | 8.423 mL |
| 5 mM | 16.847 µL | 84.233 µL | 168.466 µL | 842.332 µL | 1.685 mL |
| 10 mM | 8.423 µL | 42.117 µL | 84.233 µL | 421.166 µL | 842.332 µL |
Molarity calculator
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) | Correction Factor (482 nm) | Correction Factor (565 nm) | Correction Factor (650 nm) |
| Cyanine 3.5 amine [equivalent to Cy3.5® amine] | 579 | 591 | 150000 | 0.15 | 0.08 | 0.178 | - | - | - |
| Cyanine 5.5 bisacid [equivalent to Cy5.5® bisacid] | 683 | 703 | 250000 | 0.27 | 0.05 | 0.101 | 0.0017 | 0.047 | 0.454 |
| Cyanine 5.5 monoacid [equivalent to Cy5.5® acid] | 683 | 703 | 250000 | 0.27 | 0.05 | 0.101 | 0.0017 | 0.047 | 0.454 |
Citations
View all 15 citations: Citation Explorer
Hyaluronic acid-bilirubin nanomedicine-based combination chemoimmunotherapy
Authors: Lee, Yonghyun and Shinn, Jongyoon and Xu, Cheng and Dobson, Hannah E and Neamati, Nouri and Moon, James J
Journal: Nature Communications (2023): 4771
Authors: Lee, Yonghyun and Shinn, Jongyoon and Xu, Cheng and Dobson, Hannah E and Neamati, Nouri and Moon, James J
Journal: Nature Communications (2023): 4771
A mitochondria-targeted ratiometric fluorescent nanoprobe for imaging of peroxynitrite in living cells
Authors: Yang, Keqin and Hou, Li and Li, Zhifang and Lin, Tianran and Tian, Jianniao and Zhao, Shulin
Journal: Talanta (2021): 122421
Authors: Yang, Keqin and Hou, Li and Li, Zhifang and Lin, Tianran and Tian, Jianniao and Zhao, Shulin
Journal: Talanta (2021): 122421
Hyaluronic acid--bilirubin nanomedicine for targeted modulation of dysregulated intestinal barrier, microbiome and immune responses in colitis
Authors: Lee, Yonghyun and Sugihara, Kohei and Gillilland, Merritt G and Jon, Sangyong and Kamada, Nobuhiko and Moon, James J
Journal: Nature materials (2019): 1--9
Authors: Lee, Yonghyun and Sugihara, Kohei and Gillilland, Merritt G and Jon, Sangyong and Kamada, Nobuhiko and Moon, James J
Journal: Nature materials (2019): 1--9
Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Wang, Xiaoyan and Zhang, Yu and Xue, Wei and Wang, Hong and Qiu, Xiaozhong and Liu, Zonghua
Journal: Journal of Biomaterials Applications (2017): 923--932
Authors: Wang, Xiaoyan and Zhang, Yu and Xue, Wei and Wang, Hong and Qiu, Xiaozhong and Liu, Zonghua
Journal: Journal of Biomaterials Applications (2017): 923--932
Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)
References
View all 21 references: Citation Explorer
Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging
Authors: Berezin MY, Guo K, Akers W, Livingston J, Solomon M, Lee H, Liang K, Agee A, Achilefu S.
Journal: Biochemistry (2011): 2691
Authors: Berezin MY, Guo K, Akers W, Livingston J, Solomon M, Lee H, Liang K, Agee A, Achilefu S.
Journal: Biochemistry (2011): 2691
Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators
Authors: Freeman LM, Li S, Dayani Y, Choi HS, Malmstadt N, Armani AM.
Journal: Appl Phys Lett (2011): 143703
Authors: Freeman LM, Li S, Dayani Y, Choi HS, Malmstadt N, Armani AM.
Journal: Appl Phys Lett (2011): 143703
Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy
Authors: Wiessler M, Hennrich U, Pipkorn R, Waldeck W, Cao L, Peter J, Ehemann V, Semmler W, Lammers T, Braun K.
Journal: Theranostics (2011): 381
Authors: Wiessler M, Hennrich U, Pipkorn R, Waldeck W, Cao L, Peter J, Ehemann V, Semmler W, Lammers T, Braun K.
Journal: Theranostics (2011): 381
In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium
Authors: Adler ED, Bystrup A, Briley-Saebo KC, Mani V, Young W, Giovanonne S, Altman P, Kattman SJ, Frank JA, Weinmann HJ, Keller GM, Fayad ZA.
Journal: JACC Cardiovasc Imaging (2009): 1114
Authors: Adler ED, Bystrup A, Briley-Saebo KC, Mani V, Young W, Giovanonne S, Altman P, Kattman SJ, Frank JA, Weinmann HJ, Keller GM, Fayad ZA.
Journal: JACC Cardiovasc Imaging (2009): 1114
Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes
Authors: Volke D, Hoffmann R.
Journal: Electrophoresis (2008): 4516
Authors: Volke D, Hoffmann R.
Journal: Electrophoresis (2008): 4516