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Cyanine 5.5 monoacid [equivalent to Cy5.5® acid]

A variety of cyanine 5.5 (Cy5.5®) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc. Cy5.5® dyes are one type of the most common red fluorophores. Cy5.5® is the trademark of GE Healthcare.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cyanine 5.5 monoacid [equivalent to Cy5.5® acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM96.986 µL484.928 µL969.857 µL4.849 mL9.699 mL
5 mM19.397 µL96.986 µL193.971 µL969.857 µL1.94 mL
10 mM9.699 µL48.493 µL96.986 µL484.928 µL969.857 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)Correction Factor (650 nm)
Cyanine 5.5 bisacid [equivalent to Cy5.5® bisacid]6837032500000.270.050.1010.00170.0470.454
Cyanine 5.5 maleimide [equivalent to Cy5.5® maleimide]6837032500000.270.050.1010.00170.0470.454
Cyanine 5.5 amine [equivalent to Cy5.5® amine]6837032500000.270.050.1010.00170.0470.454
Cyanine 5.5 hydrazide [equivalent to Cy5.5® hydrazide]6837032500000.270.050.1010.00170.0470.454
Cyanine 5.5 azide [equivalent to Cy5.5® azide]6837032500000.270.050.1010.00170.0470.454
Cyanine 5.5 alkyne [equivalent to Cy5.5® alkyne]6837032500000.270.050.1010.00170.0470.454
Cyanine 7.5 monoacid [equivalent to Cy7.5® acid]785801250000------

Citations

View all 13 citations: Citation Explorer
Acidic pH-Activated Gas-Generating Nanoparticles with Pullulan Decorating for Hepatoma-Targeted Ultrasound Imaging
Authors: Chen, Shanshan and Xu, Xiao-Lin and Zhou, Boyang and Tian, Jing and Luo, Baoming and Zhang, Li-Ming
Journal: ACS Applied Materials & Interfaces (2019)
Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors: Wang, Xiaoyan and Zhang, Yu and Xue, Wei and Wang, Hong and Qiu, Xiaozhong and Liu, Zonghua
Journal: Journal of Biomaterials Applications (2017): 923--932
Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors: Guo, Fuqiang and Shang, Jiajia and Zhao, Hai and Lai, Kangrong and Li, Yang and Fan, Zhongxiong and Hou, Zhenqing and Su, Guanghao
Journal: Colloids and Surfaces B: Biointerfaces (2017)
Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors: Guo, Yuxin and Zhang, Yang and Ma, Jinyuan and Li, Qi and Li, Yang and Zhou, Xinyi and Zhao, Dan and Song, Hua and Chen, Qing and Zhu, Xuan
Journal: Journal of Controlled Release (2017)
Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors: Wang, Xu and Shi, Changying and Zhang, Li and Bodman, Alexa and Guo, D and an , undefined and Wang, Lili and Hall, Walter A and Wilkens, Stephan and Luo, Juntao
Journal: Biomaterials (2016)

References

View all 21 references: Citation Explorer
Excitation of Cy5 in self-assembled lipid bilayers using optical microresonators
Authors: Freeman LM, Li S, Dayani Y, Choi HS, Malmstadt N, Armani AM.
Journal: Appl Phys Lett (2011): 143703
Theranostic cRGD-BioShuttle Constructs Containing Temozolomide- and Cy7 For NIR-Imaging and Therapy
Authors: Wiessler M, Hennrich U, Pipkorn R, Waldeck W, Cao L, Peter J, Ehemann V, Semmler W, Lammers T, Braun K.
Journal: Theranostics (2011): 381
Rational approach to select small peptide molecular probes labeled with fluorescent cyanine dyes for in vivo optical imaging
Authors: Berezin MY, Guo K, Akers W, Livingston J, Solomon M, Lee H, Liang K, Agee A, Achilefu S.
Journal: Biochemistry (2011): 2691
In vivo detection of embryonic stem cell-derived cardiovascular progenitor cells using Cy3-labeled Gadofluorine M in murine myocardium
Authors: Adler ED, Bystrup A, Briley-Saebo KC, Mani V, Young W, Giovanonne S, Altman P, Kattman SJ, Frank JA, Weinmann HJ, Keller GM, Fayad ZA.
Journal: JACC Cardiovasc Imaging (2009): 1114
Quantitative proteomics by fluorescent labeling of cysteine residues using a set of two cyanine-based or three rhodamine-based dyes
Authors: Volke D, Hoffmann R.
Journal: Electrophoresis (2008): 4516
Page updated on December 11, 2024

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Physical properties

Molecular weight

1031.08

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.05

Correction Factor (280 nm)

0.101

Correction Factor (482 nm)

0.0017

Correction Factor (565 nm)

0.047

Correction Factor (650 nm)

0.454

Extinction coefficient (cm -1 M -1)

250000

Excitation (nm)

683

Emission (nm)

703

Quantum yield

0.27

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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