Cyanine 7.5 monoacid [equivalent to Cy7.5® acid]

Cyanine 7.5 monoacid

equivalent to Cy7.5® acid

Various cyanine dyes have been extensively used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analyses. These dyes are commonly utilized for labeling peptides, proteins, and oligos. Among them, cyanine 7.5 (Cy7.5) is a notable near-infrared dye with an exceptionally long-wave emission. Its pH insensitivity over a wide molar range (pH 3-10), makes it suitable for an extensive range of applications at biologically relevant pH levels. Moreover, the NIR emission of Cy7.5 enables deep tissue penetration, making it ideal for in vivo imaging. These dyes are DMSO-tolerant and photostable, ensuring that they can be transferred from storage to assay without any loss of performance. Our Cy7.5 dyes undergo comprehensive quality control testing to ensure high levels of chromophore and reactive dye content. For labeling reactions, mono-reactive dyes are ideal for targeted, precise labeling of proteins and oligonucleotides, while bis-reactive dyes are more suitable for general protein labeling. NHS ester dyes are recommended for labeling amine groups, and maleimide dyes are suggested for labeling thiol groups.

Cyanine 7.5 (Cy7.5) is one of the most common near-infrared (NIR) dyes for in-vivo fluorescence imaging. Its acid is an excellent building block.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
167	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Cyanine 7.5 monoacid [equivalent to Cy7.5® acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	94.598 µL	472.992 µL	945.984 µL	4.73 mL	9.46 mL
5 mM	18.92 µL	94.598 µL	189.197 µL	945.984 µL	1.892 mL
10 mM	9.46 µL	47.299 µL	94.598 µL	472.992 µL	945.984 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1057.1 g/mol	=	mL	x	mM	=	mmol

References

View all 17 references: Citation Explorer

An NIR-IIb emissive transmembrane voltage nano-indicator for the optical monitoring of electrophysiological activities in vivo.

Authors:

Xing, Zhenyu and Hu, Qian and Wang, Weikan and Kong, Na and Gao, Rong and Shen, Xiaolei and Xu, Sixin and Meng, Lingkai and Liu, Jian-Ren and Zhu, Xingjun

Journal:

Materials horizons (2024)

Theoretical Study on Spectrum and Luminescence Mechanism of Cy5.5 and Cy7.5 Dye Based on Density Functional Theory (DFT).

Authors:

Liu, Zhao and Meng, Xv and Zhang, Zhengze and Liu, Runzhang and Wang, Shutao and Lei, Jun-Qiang

Journal:

Journal of fluorescence (2023)

PSMA-targeted NIR probes for image-guided detection of prostate cancer.

Authors:

Cilibrizzi, Agostino and Wang, Julie Tzu-Wen and Memdouh, Siham and Iacovone, Antonella and McElroy, Kate and Jaffar, Noor and Young, Jennifer Denise and Hider, Robert C and Blower, Philip and Al-Jamal, Khuloud and Abbate, Vincenzo

Journal:

Colloids and surfaces. B, Biointerfaces (2022): 112734

Dual tumor- and subcellular-targeted photodynamic therapy using glucose-functionalized MoS2 nanoflakes for multidrug-resistant tumor ablation.

Authors:

Xu, Shaohui and Zhang, Pan and Heing-Becker, Isabelle and Zhang, Junmei and Tang, Peng and Bej, Raju and Bhatia, Sumati and Zhong, Yinan and Haag, Rainer

Journal:

Biomaterials (2022): 121844

Tracking the gastrointestinal digestive and metabolic behaviour of Dendrobium officinale polysaccharides by fluorescent labelling.

Authors:

Wu, Zhijing and Zhang, Yu and Nie, Gang and Liu, Junxi and Mei, Hao and He, Zihao and Dou, Pengfei and Wang, Kaiping

Journal:

Food & function (2022): 7274-7286

Physical properties

Molecular weight	1057.10
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	250000
Excitation (nm)	785
Emission (nm)	801

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on December 5, 2025