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AAT Bioquest

Cyanine 7.5 monosuccinimidyl ester [equivalent to Cy7.5® NHS ester]

Cyanine dyes are widely utilized for labeling biological molecules, particularly peptides, proteins, and oligos, in fluorescence imaging and other fluorescence-based biochemical analyses. Among these, Cyanine 7.5 (Cy7.5) NHS ester is notable for its near-infrared (NIR) properties, exhibiting long-wave emission characteristics. The NHS ester (or succinimidyl ester) is favored for its efficient reaction with the primary amines of proteins (Lys), peptides, amine-modified oligonucleotides, and other amine-containing molecules. Cy7.5 NHS Ester allows for high molar ratios of labeling without significant self-quenching, leading to brighter NIR conjugates and improved detection sensitivity. These conjugates demonstrate pH insensitivity over a wide range of molar concentrations, ensuring consistent signal generation in imaging and flow cytometry applications. Moreover, the near-infrared emission of Cy7.5 enables deep tissue penetration, making it ideal for in vivo imaging and tracking distribution in organisms. AAT Bioquest offers cyanine dye NHS esters in triethylammonium salt form, which exhibit superior solubility in dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) compared to potassium salt formulations offered by other vendors. The reactivity of the cyanine dye triethylammonium salts is equivalent to that of cyanine dye potassium salts, producing conjugates with identical properties.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)Correction Factor (482 nm)Correction Factor (565 nm)Correction Factor (650 nm)
Cyanine 3.5 monosuccinimidyl ester [equivalent to Cy3.5® NHS ester]5795911500000.150.080.178---
Cyanine 5.5 monosuccinimidyl ester [equivalent to Cy5.5® NHS ester]6837032500000.270.050.1010.00170.0470.454

References

View all 29 references: Citation Explorer
[Antitumor effects of redox-responsive nanoparticles containing platinum(Ⅳ)in ovarian cancer].
Authors: Hou, H Y and Tang, D S and Zhang, Y N and Wang, K Y and Ao, M and Luo, H X and Li, B
Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (2024): 76-85
Polysaccharide dextran-based conjugate for selective co-delivery of two synergistic drugs docetaxel and docosahexaenoic acid to tumor cells.
Authors: Dong, Peng and Lv, Hongshuai and Jia, Weiping and Liu, Jiaojiao and Wang, Si and Li, Xiaohai and Hu, Jinghua and Zhao, Ling and Shi, Yikang
Journal: Drug delivery (2023): 40-50
Theoretical Study on Spectrum and Luminescence Mechanism of Cy5.5 and Cy7.5 Dye Based on Density Functional Theory (DFT).
Authors: Liu, Zhao and Meng, Xv and Zhang, Zhengze and Liu, Runzhang and Wang, Shutao and Lei, Jun-Qiang
Journal: Journal of fluorescence (2023)
Endovascular administration of magnetized nanocarriers targeting brain delivery after stroke.
Authors: Grayston, Alba and Zhang, Yajie and Garcia-Gabilondo, Miguel and Arrúe, Mercedes and Martin, Abraham and Kopcansky, Peter and Timko, Milan and Kovac, Jozef and Strbak, Oliver and Castellote, Laura and Belloli, Sara and Moresco, Rosa M and Picchio, Maria and Roig, Anna and Rosell, Anna
Journal: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabol (2022): 237-252
PSMA-targeted NIR probes for image-guided detection of prostate cancer.
Authors: Cilibrizzi, Agostino and Wang, Julie Tzu-Wen and Memdouh, Siham and Iacovone, Antonella and McElroy, Kate and Jaffar, Noor and Young, Jennifer Denise and Hider, Robert C and Blower, Philip and Al-Jamal, Khuloud and Abbate, Vincenzo
Journal: Colloids and surfaces. B, Biointerfaces (2022): 112734
Page updated on November 5, 2024

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Catalog Number168
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Physical properties

Molecular weight

1343.73

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

250000

Excitation (nm)

785

Emission (nm)

801

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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