CypHer5E NHS Ester
CypHer5E NHS ester is an excellent building block for developing site-specific pH probes. It can be readily conjugated to amino-containing biomolecules (such as antibodies, peptides and amino-modified oligos). CypHer5E is a red laser-excitable, pH-sensitive cyanine dye derivative that can be used to study most classes of cell surface receptors which internalize into the endocytic pathway when activated by a ligand. CypHer5E NHS ester is a flexible, generic fluorophore that can be coupled to a receptor or epitope tag antibody and monitors neutral to acidic intracellular events. CypHer5E has an excitation peak that perfectly matches the red laser at 633 nm of He-Ne laser and 647 nm of red laser and an emission peak at ~670 nm.
Spectrum
Open in Advanced Spectrum Viewer
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester* | 590 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
QXY21 NHS ester [equivalent to QSY-21 NHS ester] | - | - | 890001 | - | - | 0.32 |
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester* | 553 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester* | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester* | 681 | 704 | 184000 | 0.361 | 0.00 | 0.05 |
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester* | 696 | 719 | 192000 | 0.251 | 0.00 | 0.07 |
Show More (13) |
References
View all 15 references: Citation Explorer
Altered exocytosis of inhibitory synaptic vesicles at single presynaptic terminals of cultured striatal neurons in a knock-in mouse model of Huntington's disease.
Authors: Xu, Chen and Chen, Sidong and Chen, Xingxiang and Ho, Ka Hei and Park, Chungwon and Yoo, Hanna and Lee, Suk-Ho and Park, Hyokeun
Journal: Frontiers in molecular neuroscience (2023): 1175522
Authors: Xu, Chen and Chen, Sidong and Chen, Xingxiang and Ho, Ka Hei and Park, Chungwon and Yoo, Hanna and Lee, Suk-Ho and Park, Hyokeun
Journal: Frontiers in molecular neuroscience (2023): 1175522
Measuring Phagosomal pH by Fluorescence Microscopy.
Authors: Gonzales, Gerone A and Canton, Johnathan
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 153-169
Authors: Gonzales, Gerone A and Canton, Johnathan
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 153-169
A near-infrared fluorescent pH sensing film for wound milieu pH monitoring.
Authors: Li, Shuxin and Vu, Hong and Senkowsky, Jon and Hu, Wenjing and Tang, Liping
Journal: Experimental dermatology (2020): 107-111
Authors: Li, Shuxin and Vu, Hong and Senkowsky, Jon and Hu, Wenjing and Tang, Liping
Journal: Experimental dermatology (2020): 107-111
RNAi in Spodoptera frugiperda Sf9 Cells via Nanomaterial Mediated Delivery of dsRNA: A Comparison of Poly-l-arginine Polyplexes and Poly-l-arginine-Functionalized Au Nanoparticles.
Authors: Laisney, Jérôme and Gurusamy, Dhandapani and Baddar, Zeinah Elhaj and Palli, Subba Reddy and Unrine, Jason M
Journal: ACS applied materials & interfaces (2020): 25645-25657
Authors: Laisney, Jérôme and Gurusamy, Dhandapani and Baddar, Zeinah Elhaj and Palli, Subba Reddy and Unrine, Jason M
Journal: ACS applied materials & interfaces (2020): 25645-25657
Transport of orally delivered dsRNA in southern green stink bug, Nezara viridula.
Authors: Gurusamy, Dhandapani and Howell, Jeffrey L and Chereddy, Shankar C R R and Koo, Jinmo and Palli, Subba Reddy
Journal: Archives of insect biochemistry and physiology (2020): e21692
Authors: Gurusamy, Dhandapani and Howell, Jeffrey L and Chereddy, Shankar C R R and Koo, Jinmo and Palli, Subba Reddy
Journal: Archives of insect biochemistry and physiology (2020): e21692
Page updated on November 27, 2024