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CypHer5E NHS Ester
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 827.02 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 643 |
| Emission (nm) | 660 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
Molecular weight 827.02 | Excitation (nm) 643 | Emission (nm) 660 |
CypHer5E NHS ester is an excellent building block for developing site-specific pH probes. It can be readily conjugated to amino-containing biomolecules (such as antibodies, peptides and amino-modified oligos). CypHer5E is a red laser-excitable, pH-sensitive cyanine dye derivative that can be used to study most classes of cell surface receptors which internalize into the endocytic pathway when activated by a ligand. CypHer5E NHS ester is a flexible, generic fluorophore that can be coupled to a receptor or epitope tag antibody and monitors neutral to acidic intracellular events. CypHer5E has an excitation peak that perfectly matches the red laser at 633 nm of He-Ne laser and 647 nm of red laser and an emission peak at ~670 nm.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CypHer5E NHS Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 120.916 µL | 604.58 µL | 1.209 mL | 6.046 mL | 12.092 mL |
| 5 mM | 24.183 µL | 120.916 µL | 241.832 µL | 1.209 mL | 2.418 mL |
| 10 mM | 12.092 µL | 60.458 µL | 120.916 µL | 604.58 µL | 1.209 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester* | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
| XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester* | 343 | 441 | 19000 | - | 0.25 | 0.19 |
| XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester* | 534 | 553 | 81000 | 0.611 | 0.24 | 0.09 |
| XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester* | 590 | 618 | 90000 | 0.661 | 0.43 | 0.56 |
| QXY21 NHS ester [equivalent to QSY-21 NHS ester] | - | - | 890001 | - | - | 0.32 |
| XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester* | 553 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
| XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester* | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
| XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester* | 681 | 704 | 184000 | 0.361 | 0.00 | 0.05 |
| XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester* | 696 | 719 | 192000 | 0.251 | 0.00 | 0.07 |
Show More (10) | ||||||
Images
Figure 1. CypHer5E NHS ester is an excellent building block for developing site-specific pH probes. It can be readily conjugated to amino-containing biomolecules (such as antibodies, peptides and amino-modified oligos).
References
View all 15 references: Citation Explorer
Measuring Phagosomal pH by Fluorescence Microscopy.
Authors: Gonzales, Gerone A and Canton, Johnathan
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 153-169
Authors: Gonzales, Gerone A and Canton, Johnathan
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 153-169
Altered exocytosis of inhibitory synaptic vesicles at single presynaptic terminals of cultured striatal neurons in a knock-in mouse model of Huntington's disease.
Authors: Xu, Chen and Chen, Sidong and Chen, Xingxiang and Ho, Ka Hei and Park, Chungwon and Yoo, Hanna and Lee, Suk-Ho and Park, Hyokeun
Journal: Frontiers in molecular neuroscience (2023): 1175522
Authors: Xu, Chen and Chen, Sidong and Chen, Xingxiang and Ho, Ka Hei and Park, Chungwon and Yoo, Hanna and Lee, Suk-Ho and Park, Hyokeun
Journal: Frontiers in molecular neuroscience (2023): 1175522
Transport of orally delivered dsRNA in southern green stink bug, Nezara viridula.
Authors: Gurusamy, Dhandapani and Howell, Jeffrey L and Chereddy, Shankar C R R and Koo, Jinmo and Palli, Subba Reddy
Journal: Archives of insect biochemistry and physiology (2020): e21692
Authors: Gurusamy, Dhandapani and Howell, Jeffrey L and Chereddy, Shankar C R R and Koo, Jinmo and Palli, Subba Reddy
Journal: Archives of insect biochemistry and physiology (2020): e21692
RNAi in Spodoptera frugiperda Sf9 Cells via Nanomaterial Mediated Delivery of dsRNA: A Comparison of Poly-l-arginine Polyplexes and Poly-l-arginine-Functionalized Au Nanoparticles.
Authors: Laisney, Jérôme and Gurusamy, Dhandapani and Baddar, Zeinah Elhaj and Palli, Subba Reddy and Unrine, Jason M
Journal: ACS applied materials & interfaces (2020): 25645-25657
Authors: Laisney, Jérôme and Gurusamy, Dhandapani and Baddar, Zeinah Elhaj and Palli, Subba Reddy and Unrine, Jason M
Journal: ACS applied materials & interfaces (2020): 25645-25657
A near-infrared fluorescent pH sensing film for wound milieu pH monitoring.
Authors: Li, Shuxin and Vu, Hong and Senkowsky, Jon and Hu, Wenjing and Tang, Liping
Journal: Experimental dermatology (2020): 107-111
Authors: Li, Shuxin and Vu, Hong and Senkowsky, Jon and Hu, Wenjing and Tang, Liping
Journal: Experimental dermatology (2020): 107-111
High-Throughput Screening for Internalizing Antibodies by Homogeneous Fluorescence Imaging of a pH-Activated Probe.
Authors: Riedl, Thilo and van Boxtel, Egon and Bosch, Martijn and Parren, Paul W H I and Gerritsen, Arnout F
Journal: Journal of biomolecular screening (2016): 12-23
Authors: Riedl, Thilo and van Boxtel, Egon and Bosch, Martijn and Parren, Paul W H I and Gerritsen, Arnout F
Journal: Journal of biomolecular screening (2016): 12-23
Distinct modes of endocytotic presynaptic membrane and protein uptake at the calyx of Held terminal of rats and mice.
Authors: Okamoto, Yuji and Lipstein, Noa and Hua, Yunfeng and Lin, Kun-Han and Brose, Nils and Sakaba, Takeshi and Midorikawa, Mitsuharu
Journal: eLife (2016)
Authors: Okamoto, Yuji and Lipstein, Noa and Hua, Yunfeng and Lin, Kun-Han and Brose, Nils and Sakaba, Takeshi and Midorikawa, Mitsuharu
Journal: eLife (2016)
Real-time noninvasive monitoring of in vivo inflammatory responses using a pH ratiometric fluorescence imaging probe.
Authors: Tsai, Yi-Ting and Zhou, Jun and Weng, Hong and Shen, Jinhui and Tang, Liping and Hu, Wen-Jing
Journal: Advanced healthcare materials (2014): 221-9
Authors: Tsai, Yi-Ting and Zhou, Jun and Weng, Hong and Shen, Jinhui and Tang, Liping and Hu, Wen-Jing
Journal: Advanced healthcare materials (2014): 221-9
The antidepressant fluoxetine mobilizes vesicles to the recycling pool of rat hippocampal synapses during high activity.
Authors: Jung, Jasmin and Loy, Kristina and Schilling, Eva-Maria and Röther, Mareike and Brauner, Jan M and Huth, Tobias and Schlötzer-Schrehardt, Ursula and Alzheimer, Christian and Kornhuber, Johannes and Welzel, Oliver and Groemer, Teja W
Journal: Molecular neurobiology (2014): 916-30
Authors: Jung, Jasmin and Loy, Kristina and Schilling, Eva-Maria and Röther, Mareike and Brauner, Jan M and Huth, Tobias and Schlötzer-Schrehardt, Ursula and Alzheimer, Christian and Kornhuber, Johannes and Welzel, Oliver and Groemer, Teja W
Journal: Molecular neurobiology (2014): 916-30
The pH probe CypHer™5E is effectively quenched by FM dyes.
Authors: Welzel, Oliver and Loy, Kristina and Tischbirek, Carsten H and Tabor, Alina and Gmeiner, Peter and Kornhuber, Johannes and Groemer, Teja W
Journal: Journal of fluorescence (2013): 487-94
Authors: Welzel, Oliver and Loy, Kristina and Tischbirek, Carsten H and Tabor, Alina and Gmeiner, Peter and Kornhuber, Johannes and Groemer, Teja W
Journal: Journal of fluorescence (2013): 487-94