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CYTO-13 *5 mM DMSO solution*

CYTO-13 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. CYTO-13 has its green fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of live cells with proper filter sets. Based on our in-house evaluations, it has equivalent DNA staining properties almost identical to SYTO-13™ DNA stain (SYTO-13™ is the trade mark of ThermoFisher).

Spectrum

Citations

View all 2 citations: Citation Explorer
Evaluation of an Alternative Staining Method Using SYTO 13 to Determine Reticulated Platelets
Authors: Hille, L., Cederqvist, M., Hromek, J., Stratz, C., Trenk, D., Nuhrenberg, T. G.
Journal: Thromb Haemost (2019): 779-785
SYTO-13, a Viability Marker as a New Tool to Monitor In Vitro Pharmacodynamic Parameters of Anti-Pneumocystis Drugs
Authors: St, undefined and aert-Vitse, A., Aliouat-Denis, C. M., Martinez, A., Khalife, S., Pottier, M., Gantois, N., Dei-Cas, E., Aliouat el, M.
Journal: PLoS One (2015): e0130358

References

View all 1 references: Citation Explorer
Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry
Authors: Guindulain T, Comas J, Vives-Rego J.
Journal: Appl Environ Microbiol (1997): 4608
Page updated on November 3, 2024

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Catalog Number17553
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Physical properties

Molecular weight

524.42

Solvent

DMSO

Spectral properties

Excitation (nm)

486

Emission (nm)

511

Storage, safety and handling

Intended useResearch Use Only (RUO)

Storage

Freeze (< -15 °C); Minimize light exposure

Platform

Fluorescence microscope

ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall, clear bottom
Instrument specification(s)FITC filter set
<strong>CYTO-13 </strong><strong>live-cell nucleic acid stain</strong><strong>.</strong><br>Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37&deg;C, washed once with HH buffer, and then imaged with FITC filter.
<strong>CYTO-13 </strong><strong>live-cell nucleic acid stain</strong><strong>.</strong><br>Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37&deg;C, washed once with HH buffer, and then imaged with FITC filter.
<strong>CYTO-13 </strong><strong>live-cell nucleic acid stain</strong><strong>.</strong><br>Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37&deg;C, washed once with HH buffer, and then imaged with FITC filter.
<strong>CYTO-13 live-cell nucleic acid stain.</strong><br>Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37&deg;C, and washed once with HH buffer. The photo was acquired with FITC&nbsp; filter set and differential interference contrast (DIC) sequentially in a fluorescence microscope.

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