CYTO-13 *5 mM DMSO solution*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 524.42 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 486 |
| Emission (nm) | 511 |
Storage, safety and handling
| Intended use | Research Use Only (RUO) |
| Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
| CYTO-13 |
| Overview |  SDSProtocol |
Molecular weight 524.42 | Excitation (nm) 486 | Emission (nm) 511 |
CYTO-13 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. CYTO-13 has its green fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of live cells with proper filter sets. Based on our in-house evaluations, it has equivalent DNA staining properties almost identical to SYTO-13™ DNA stain (SYTO-13™ is the trade mark of ThermoFisher).
Platform
Fluorescence microscope
| Excitation | FITC filter set |
| Emission | FITC filter set |
| Recommended plate | Black wall/clear bottom |
| Instrument specification(s) | FITC filter set |
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CYTO-13 *5 mM DMSO solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 190.687 µL | 953.434 µL | 1.907 mL | 9.534 mL | 19.069 mL |
| 5 mM | 38.137 µL | 190.687 µL | 381.374 µL | 1.907 mL | 3.814 mL |
| 10 mM | 19.069 µL | 95.343 µL | 190.687 µL | 953.434 µL | 1.907 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. CYTO-13 live-cell nucleic acid stain.
Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37°C, washed once with HH buffer, and then imaged with FITC filter.
Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37°C, washed once with HH buffer, and then imaged with FITC filter.
Figure 2. CYTO-13 live-cell nucleic acid stain.
Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37°C, and washed once with HH buffer. The photo was acquired with FITC filter set and differential interference contrast (DIC) sequentially in a fluorescence microscope.
Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37°C, and washed once with HH buffer. The photo was acquired with FITC filter set and differential interference contrast (DIC) sequentially in a fluorescence microscope.
Citations
View all 2 citations: Citation Explorer
Evaluation of an Alternative Staining Method Using SYTO 13 to Determine Reticulated Platelets
Authors: Hille, L., Cederqvist, M., Hromek, J., Stratz, C., Trenk, D., Nuhrenberg, T. G.
Journal: Thromb Haemost (2019): 779-785
Authors: Hille, L., Cederqvist, M., Hromek, J., Stratz, C., Trenk, D., Nuhrenberg, T. G.
Journal: Thromb Haemost (2019): 779-785
SYTO-13, a Viability Marker as a New Tool to Monitor In Vitro Pharmacodynamic Parameters of Anti-Pneumocystis Drugs
Authors: St, undefined and aert-Vitse, A., Aliouat-Denis, C. M., Martinez, A., Khalife, S., Pottier, M., Gantois, N., Dei-Cas, E., Aliouat el, M.
Journal: PLoS One (2015): e0130358
Authors: St, undefined and aert-Vitse, A., Aliouat-Denis, C. M., Martinez, A., Khalife, S., Pottier, M., Gantois, N., Dei-Cas, E., Aliouat el, M.
Journal: PLoS One (2015): e0130358
References
View all 1 references: Citation Explorer
Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry
Authors: Guindulain T, Comas J, Vives-Rego J.
Journal: Appl Environ Microbiol (1997): 4608
Authors: Guindulain T, Comas J, Vives-Rego J.
Journal: Appl Environ Microbiol (1997): 4608