CYTO-13 5 mM DMSO solution

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Physical properties

Molecular weight	524.42
Solvent	DMSO

Spectral properties

Excitation (nm)	486
Emission (nm)	511

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Alternative formats

Overview SDS Protocol

Molecular weight

524.42

Excitation (nm)

486

Emission (nm)

511

CYTO-13 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. CYTO-13 has its green fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of live cells with proper filter sets. Based on our in-house evaluations, it has equivalent DNA staining properties almost identical to SYTO-13™ DNA stain (SYTO-13™ is the trade mark of ThermoFisher).

Platform

Fluorescence microscope

Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC filter set

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CYTO-13 *5 mM DMSO solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	190.687 µL	953.434 µL	1.907 mL	9.534 mL	19.069 mL
5 mM	38.137 µL	190.687 µL	381.374 µL	1.907 mL	3.814 mL
10 mM	19.069 µL	95.343 µL	190.687 µL	953.434 µL	1.907 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	486
Emission (nm)	511

Images

Figure 1. CYTO-13 live-cell nucleic acid stain.
Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37°C, washed once with HH buffer, and then imaged with FITC filter.

Figure 2. CYTO-13 live-cell nucleic acid stain.
Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37°C, and washed once with HH buffer. The photo was acquired with FITC filter set and differential interference contrast (DIC) sequentially in a fluorescence microscope.

Citations

View all 2 citations: Citation Explorer

Evaluation of an Alternative Staining Method Using SYTO 13 to Determine Reticulated Platelets
Authors: Hille, L., Cederqvist, M., Hromek, J., Stratz, C., Trenk, D., Nuhrenberg, T. G.
Journal: Thromb Haemost (2019): 779-785

SYTO-13, a Viability Marker as a New Tool to Monitor In Vitro Pharmacodynamic Parameters of Anti-Pneumocystis Drugs
Authors: St, undefined and aert-Vitse, A., Aliouat-Denis, C. M., Martinez, A., Khalife, S., Pottier, M., Gantois, N., Dei-Cas, E., Aliouat el, M.
Journal: PLoS One (2015): e0130358

References

View all 1 references: Citation Explorer

Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry
Authors: Guindulain T, Comas J, Vives-Rego J.
Journal: Appl Environ Microbiol (1997): 4608