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CYTO-13 *5 mM DMSO solution*

<strong>CYTO-13 </strong><strong>live-cell nucleic acid stain</strong><strong>.</strong><br>Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37°C, washed once with HH buffer, and then imaged with FITC filter.
<strong>CYTO-13 </strong><strong>live-cell nucleic acid stain</strong><strong>.</strong><br>Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37°C, washed once with HH buffer, and then imaged with FITC filter.
<strong>CYTO-13 live-cell nucleic acid stain.</strong><br>Live HeLa cells were stained with 2.5uM CYTO-13 (Cat# 17553) for 20 minutes at 37°C, and washed once with HH buffer. The photo was acquired with FITC  filter set and differential interference contrast (DIC) sequentially in a fluorescence microscope.
Ordering information
Price ()
Catalog Number17553
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight524.42
SolventDMSO
Spectral properties
Excitation (nm)486
Emission (nm)511
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
Alternative formats
CYTO-13

OverviewpdfSDSpdfProtocol


Molecular weight
524.42
Excitation (nm)
486
Emission (nm)
511
CYTO-13 is a fluorogenic, DNA-selective and cell-permeant dye for analyzing DNA content in living cells. CYTO-13 has its green fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of live cells with proper filter sets. Based on our in-house evaluations, it has equivalent DNA staining properties almost identical to SYTO-13™ DNA stain (SYTO-13™ is the trade mark of ThermoFisher).

Platform


Fluorescence microscope

ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC filter set

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CYTO-13 *5 mM DMSO solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM190.687 µL953.434 µL1.907 mL9.534 mL19.069 mL
5 mM38.137 µL190.687 µL381.374 µL1.907 mL3.814 mL
10 mM19.069 µL95.343 µL190.687 µL953.434 µL1.907 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Excitation (nm)486
Emission (nm)511

Citations


View all 2 citations: Citation Explorer
Evaluation of an Alternative Staining Method Using SYTO 13 to Determine Reticulated Platelets
Authors: Hille, L., Cederqvist, M., Hromek, J., Stratz, C., Trenk, D., Nuhrenberg, T. G.
Journal: Thromb Haemost (2019): 779-785
SYTO-13, a Viability Marker as a New Tool to Monitor In Vitro Pharmacodynamic Parameters of Anti-Pneumocystis Drugs
Authors: St, undefined and aert-Vitse, A., Aliouat-Denis, C. M., Martinez, A., Khalife, S., Pottier, M., Gantois, N., Dei-Cas, E., Aliouat el, M.
Journal: PLoS One (2015): e0130358

References


View all 1 references: Citation Explorer
Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry
Authors: Guindulain T, Comas J, Vives-Rego J.
Journal: Appl Environ Microbiol (1997): 4608