Name: CYTO-16 *equivalent to SYTO 16*
Brand: AAT Bioquest
SKU: 17568
Price: 237 USD
Availability: InStock

CYTO-16

equivalent to SYTO 16

CYTO 16 (SYTO 16 equivalent) is a membrane-permeable green fluorescent nucleic acid stain that provides exceptional fluorescence enhancement upon binding, enabling sensitive detection of nucleic acids in both mammalian cells and bacteria.

Green Fluorescent Nucleic Acid Stain: Ex/Em = 488/518 nm (DNA) and 494/525 nm (RNA) with >100-fold fluorescence enhancement upon binding
Cell-Permeant Design: Penetrates both mammalian cell membranes and bacterial cell walls for live-cell imaging
Direct SYTO 16 Replacement: Identical spectral properties and staining patterns with equivalent binding affinity
Multi-Compartment Staining: Labels nuclear DNA, mitochondrial nucleoids, and cytoplasmic RNA in eukaryotic cells

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
17568	0.5 mL	Price

Overview

CYTO 16 is manufactured by AAT Bioquest to replace SYTO 16 Green Nucleic Acid Stain. They have virtually the same chemical and biological properties. CYTO 16 is a cell-permeant nucleic acid stain that exhibits bright, green fluorescence upon binding to nucleic acids. The CYTO 16 dye can be used to stain RNA (Ex/Em ~ 494/525 nm) and DNA (Ex/Em ~ 488/518 nm) in both live and dead eukaryotic cells, as well as in Gram-positive and Gram-negative bacteria. Eukaryotic cells incubated with SYTO 16 dye generally show cytoplasmic or mitochondrial staining, as well as nuclear staining.

References

View all 44 references: Citation Explorer

Evaluation of Semen Self-Sampling Yield Predictors and CTC Isolation by Multi-Color Flow Cytometry for Liquid Biopsy of Localized Prostate Cancer.

Authors:

Saitta, Cesare and De Simone, Ilaria and Fasulo, Vittorio and Corbetta, Marinella and Duga, Stefano and Chiereghin, Chiara and Colombo, Federico Simone and Benetti, Alessio and Contieri, Roberto and Avolio, Pier Paolo and Uleri, Alessandro and Saita, Alberto and Guazzoni, Giorgio Ferruccio and Hurle, Rodolfo and Colombo, Piergiuseppe and Buffi, Nicolò Maria and Casale, Paolo and Lughezzani, Giovanni and Asselta, Rosanna and Soldà, Giulia and Lazzeri, Massimo

Journal:

Cancers (2023)

Dual DNA staining enables isolation of multiple sub-types of post-replicative mouse male germ cells.

Authors:

Gill, Mark E and Kohler, Hubertus and Peters, Antoine H F M

Journal:

Cytometry. Part A : the journal of the International Society for Analytical Cytology (2022): 529-536

Chemical fluorescence-based dye staining for 3-dimensional histopathology analysis.

Authors:

Kim, Hyun Jung and Kim, Jeongah and Choi, Jungyoon and Sun, Woong

Journal:

Animal cells and systems (2022): 45-51

Analysis of extracellular vesicle DNA at the single-vesicle level by nano-flow cytometry.

Authors:

Liu, Haisheng and Tian, Ye and Xue, Chengfeng and Niu, Qian and Chen, Chen and Yan, Xiaomei

Journal:

Journal of extracellular vesicles (2022): e12206

Salvadora persica mediated synthesis of silver nanoparticles and their antimicrobial efficacy.

Authors:

Arshad, Hammad and Sami, Muhammad A and Sadaf, Saima and Hassan, Umer

Journal:

Scientific reports (2021): 5996

Physical properties

Molecular weight	565.52
Solvent	DMSO

Spectral properties

Excitation (nm)	489
Emission (nm)	520

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on April 2, 2026