CytoALL™ DNA Red 600

The common fluorescent DNA probes (such as DAPI, Hoechst or SYBR® Green) predominantly stains the DNAs in nuclei. There is a unmet need for a universal DNA probe that can stain the total cellular DNAs in live cells. CytoALL™ DNA Red 600 has been developed to stain all the DNAs in live cells. CytoALL™ DNA Red 600 is a DNA-selective, cell permeable dye that stains all DNA contents including nuclei and mitochondria in live cells. The staining is simple and robust. It only requires a single step of incubating cells in the presence of CytoALL™ DNA Red 600. The cells can be viewed with a TRITC filter set, allowing it to multiplex with various other fluorescent imaging probes. AAT Bioquest also offers a variety of DNA probes for staining nuclei (such as Nuclear Blue™, Nuclear Green™ and Nuclear Red™) and MitoDNA™ for selective staining of mitochondrial DNAs (mtDNA).

Protocol

SDS

Catalog	Size	Price	Quantity
22270	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoALL™ DNA Red 600 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	244.248 µL	1.221 mL	2.442 mL	12.212 mL	24.425 mL
5 mM	48.85 µL	244.248 µL	488.496 µL	2.442 mL	4.885 mL
10 mM	24.425 µL	122.124 µL	244.248 µL	1.221 mL	2.442 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	409.42 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Tag-free fluorometric aptasensor for detection of chromium(VI) in foods via SYBR Green I signal amplification and aptamer structure transition.

Authors:

Zhu, Jiangxiong and Yin, Hao and Zheng, Sisi and Yu, Hong and Yang, Linnan and Wang, Lumei and Geng, Xueqing and Deng, Yun

Journal:

Journal of the science of food and agriculture (2024)

Detection of SARS-CoV-2 B.1.1.529 (Omicron) variant by SYBR Green-based RT-qPCR.

Authors:

Abdel-Sater, Fadi and Makki, Rawan and Khalil, Alia and Hussein, Nader and Borghol, Nada and Abi Khattar, Ziad and Hamade, Aline and Khreich, Nathalie and El Homsi, Mahoumd and Kanaan, Hussein and Raad, Line and Skafi, Najwa and Al-Nemer, Fatima and Ghandour, Zeinab and El-Zein, Nabil and Abou-Hamdan, Mhamad and Akl, Haidar and Hamade, Eva and Badran, Bassam and Hamze, Kassem

Journal:

Biology methods & protocols (2024): bpae020

Development of a fast and sensitive RT-qPCR assay based on SYBR® green for diagnostic and quantification of Avian Nephritis Virus (ANV) in chickens affected with enteric disease.

Authors:

Loor-Giler, Anthony and Castillo-Reyes, Sara and Santander-Parra, Silvana and Caza, Manuel and Kyriakidis, Nikolaos C and Ferreira, Antonio J Piantino and Nuñez, Luis

Journal:

BMC veterinary research (2024): 33

A label free fluorescent aptamer sensor based on the combined action of Graphene oxide and SYBR Green I for the detection of Aflatoxin B1.

Authors:

Hu, Mengyang and Cheng, Meng and Wang, Na and Sang, Yidan and Dong, Yafei and Wang, Luhui

Journal:

IEEE transactions on nanobioscience (2024)

The Significance and Importance of dPCR, qPCR, and SYBR Green PCR Kit in the Detection of Numerous Diseases.

Authors:

Shahrajabian, Mohamad Hesam and Sun, Wenli

Journal:

Current pharmaceutical design (2024): 169-179

Physical properties

Molecular weight	409.42
Solvent	DMSO

Spectral properties

Excitation (nm)	582
Emission (nm)	598

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	Cy3 Filter Set
Emission	Cy3 Filter Set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on May 5, 2026