CytoALL™ DNA Red 600

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1. Prepare the cells in growth medium.

2. Stain the cells with CytoALL™ DNA Red 600 working solution.

3. Incubate at 37 °C for 5-15 minutes.

4. Use a fluorescence microscope with a Cy3 filter set to monitor the fluorescence intensity.

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles

1. Prepare a 5 to 10 mM CytoALL™ DNA Red 600 stock solution in DMSO. For example, to make a 10 mM stock solution add 245 μL of DMSO to the vial of CytoALL™ DNA Red 600, and mix well.

   Note: Prepare single-use aliquots of the CytoALL™ DNA Red 600 stock solution and store at ≤ -20°C, protected from light. Avoid repeated freeze-thaw cycles.

1. Prepare a 10 to 20 μM working solution by diluting the CytoALL™ DNA Red 600 stock solution with Hanks solution containing 20 mM Hepes buffer (HHBS).

   Note: For optimal results, use this solution within a few hours of preparation.

   Note: Protect the CytoALL™ DNA Red 600 working solution from light by covering it with foil or storing it in a dark place.

1. Plate cells as desired in a 96-well black wall-clear bottom plate.

2. Remove the cell culture medium and add 100 µL of CytoALL™ DNA Red 600 working solution to the cells.

3. Incubate the cells at 37 º C for 5 to 15 minutes, protected from light.

   Note: The optimal concentration and incubation time for CytoALL™ DNA Red 600 vary depending on the cell line. It is necessary to test different concentrations to determine the most effective dose for your specific cell line.

4. Remove the dye working solution and wash the cells twice with HHBS buffer.

5. Add HHBS buffer and analyze the cells with the fluorescence microscope using a Cy3 filter set.