CytoFix™ BCECF, AM *Optimized for long term cellular pH tracking*

CytoFix™ BCECF, AM

Optimized for long term cellular pH tracking

Intracellular pH plays an important modulating role in many cellular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, cell adhesion and other cellular processes. pH-sensitive fluorescent dyes have been widely applied to monitor changes in intracellular pH in recent years. Imaging techniques that use fluorescent pH indicators also allow researchers to investigate these processes with much greater spatial resolution and sampling density that can be achieved using other technologies such as microelectrode. Among them, 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) is the most popular pH probe since it can be used to monitor cellular pH ratiometrically with pKa ~7.0. BCECF AM is the cell-permeable version of BCECF. However, BCECF rapidly leaks out of lives cell when BCECF is loaded into live cells through its AM ester due to the activities of drug efflux transporters. CytoFix™ BCECF is a modified BCECF derivative that contains a cell-retention group. The newly modified BCECF derivative is well retained in live cells for much longer time than the parent BCECF dye. CytoFix™ BCECF retains the same pH profile of BCECF with improved cell retention.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21200	1 mg	Price

Citations

View all 3 citations: Citation Explorer

Intracellular pH Measurements in Glioblastoma Cells Using the pH-Sensitive Dye BCECF

Authors:

Galifianakis, N. V., Placantonakis, D. G., Chesler, M.

Journal:

Methods Mol Biol (2018): 103-109

Evaluation of BCECF fluorescence ratio imaging to properly measure gastric intramucosal pH variations in vivo

Authors:

Rochon, P., Jourdain, M., Mangalaboyi, J., Fourrier, F., Soulie-Begu, S., Buys, B., Dehlin, G., Lesage, J. C., Chambrin, M. C., Mordon, S.

Journal:

J Biomed Opt (2007): 064014

Calibration methods and avoidance of errors in measurement of intracellular pH (pHcyt) using the indicator bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in human platelets

Authors:

Valant, P. A., Haynes, D. H.

Journal:

J Fluoresc (1992): 191-206

Physical properties

Molecular weight	638.63
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	90000
Excitation (nm)	504
Emission (nm)	527

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 15, 2025