CytoFix™ BCECF, AM *Optimized for long term cellular pH tracking*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 638.63 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 90000 |
Excitation (nm) | 504 |
Emission (nm) | 527 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
CytoFix™ Red Mitochondrial Stain |
CytoFix™ Red Lysosomal Stain |
Overview | ![]() ![]() |
See also: Fluoresceins, Intracellular pH
Molecular weight 638.63 | Extinction coefficient (cm -1 M -1) 90000 | Excitation (nm) 504 | Emission (nm) 527 |
Intracellular pH plays an important modulating role in many cellular events, including cell growth, calcium regulation, enzymatic activity, receptor-mediated signal transduction, ion transport, endocytosis, chemotaxis, cell adhesion and other cellular processes. pH-sensitive fluorescent dyes have been widely applied to monitor changes in intracellular pH in recent years. Imaging techniques that use fluorescent pH indicators also allow researchers to investigate these processes with much greater spatial resolution and sampling density that can be achieved using other technologies such as microelectrode. Among them, 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein (BCECF) is the most popular pH probe since it can be used to monitor cellular pH ratiometrically with pKa ~7.0. BCECF AM is the cell-permeable version of BCECF. However, BCECF rapidly leaks out of lives cell when BCECF is loaded into live cells through its AM ester due to the activities of drug efflux transporters. CytoFix™ BCECF is a modified BCECF derivative that contains a cell-retention group. The newly modified BCECF derivative is well retained in live cells for much longer time than the parent BCECF dye. CytoFix™ BCECF retains the same pH profile of BCECF with improved cell retention.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CytoFix™ BCECF, AM *Optimized for long term cellular pH tracking* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 156.585 µL | 782.926 µL | 1.566 mL | 7.829 mL | 15.659 mL |
5 mM | 31.317 µL | 156.585 µL | 313.17 µL | 1.566 mL | 3.132 mL |
10 mM | 15.659 µL | 78.293 µL | 156.585 µL | 782.926 µL | 1.566 mL |
Molarity calculator
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Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 90000 |
Excitation (nm) | 504 |
Emission (nm) | 527 |
Citations
View all 3 citations: Citation Explorer
Intracellular pH Measurements in Glioblastoma Cells Using the pH-Sensitive Dye BCECF
Authors: Galifianakis, N. V., Placantonakis, D. G., Chesler, M.
Journal: Methods Mol Biol (2018): 103-109
Authors: Galifianakis, N. V., Placantonakis, D. G., Chesler, M.
Journal: Methods Mol Biol (2018): 103-109
Evaluation of BCECF fluorescence ratio imaging to properly measure gastric intramucosal pH variations in vivo
Authors: Rochon, P., Jourdain, M., Mangalaboyi, J., Fourrier, F., Soulie-Begu, S., Buys, B., Dehlin, G., Lesage, J. C., Chambrin, M. C., Mordon, S.
Journal: J Biomed Opt (2007): 064014
Authors: Rochon, P., Jourdain, M., Mangalaboyi, J., Fourrier, F., Soulie-Begu, S., Buys, B., Dehlin, G., Lesage, J. C., Chambrin, M. C., Mordon, S.
Journal: J Biomed Opt (2007): 064014
Calibration methods and avoidance of errors in measurement of intracellular pH (pHcyt) using the indicator bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) in human platelets
Authors: Valant, P. A., Haynes, D. H.
Journal: J Fluoresc (1992): 191-206
Authors: Valant, P. A., Haynes, D. H.
Journal: J Fluoresc (1992): 191-206
References
View all 1 references: Citation Explorer
Estimation of intramitochondrial pca and ph by fura-2 and 2,7 biscarboxyethyl-5(6)-carboxyfluorescein (bcecf) fluorescence
Authors: Davis MH, Altschuld RA, Jung DW, Brierley GP.
Journal: Biochem Biophys Res Commun (1987): 40
Authors: Davis MH, Altschuld RA, Jung DW, Brierley GP.
Journal: Biochem Biophys Res Commun (1987): 40
Application notes
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A Meta-Analysis of Common Calcium Indicators
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A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets