CytoFix™ Red Lysosomal Stain
Ordering information
Price | |
Catalog Number | |
Unit Size | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 863.75 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 565 |
Emission (nm) | 585 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Lysosomes
CAS N/A | Molecular weight 863.75 | Excitation (nm) 565 | Emission (nm) 585 |
Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste and cellular debris through a process known as autophagy. AAT Bioquest offers CytoFix™ Red lysosomal stain for selectively staining lysosomes. CytoFix™ Red lysosomal stain is well retained in lysosomes even after fixation. The dye permeates intact live cells and gets trapped in lysosomes. The fluorescence in lysosomes generated by this dye is well retained at least for 1 week, making it an excellent lysosomal tracking dye. The key features of this stain are its high staining efficiency, long retention after fixation with minimal hands on time. CytoFix™ Red lysosomal stain can be used with GFP expressed cells or with other organelles stains for multicolor analysis. It can be used for both suspension and adherent cells and readily adapted for a wide variety of fluorescence platforms.
Platform
Fluorescence microscope
Excitation | Cy3/TRITC filter set |
Emission | Cy3/TRITC filter set |
Recommended plate | Black wall/clear bottom |
Instrument specification(s) | Cy3/TRITC filter set |
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CytoFix™ Red Lysosomal Stain to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 115.774 µL | 578.871 µL | 1.158 mL | 5.789 mL | 11.577 mL |
5 mM | 23.155 µL | 115.774 µL | 231.548 µL | 1.158 mL | 2.315 mL |
10 mM | 11.577 µL | 57.887 µL | 115.774 µL | 578.871 µL | 1.158 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. The fluorescence images of HeLa cells stained with CytoFix™ LysoRed in a 96-well black-wall clear-bottom plate. Image was acquired before (Left) and after (Right) fixation with 4% formaldehyde solution for 20 minutes at RT. The cells were imaged using fluorescence microscope with a Cy3/TRITC filter.
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?