CytoFix™ Red Lysosomal Stain

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	863.75
Solvent	DMSO

Spectral properties

Excitation (nm)	565
Emission (nm)	585

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

See also: Lysosomes

CAS

N/A

Molecular weight

863.75

Excitation (nm)

565

Emission (nm)

585

Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste and cellular debris through a process known as autophagy. AAT Bioquest offers CytoFix™ Red lysosomal stain for selectively staining lysosomes. CytoFix™ Red lysosomal stain is well retained in lysosomes even after fixation. The dye permeates intact live cells and gets trapped in lysosomes. The fluorescence in lysosomes generated by this dye is well retained at least for 1 week, making it an excellent lysosomal tracking dye. The key features of this stain are its high staining efficiency, long retention after fixation with minimal hands on time. CytoFix™ Red lysosomal stain can be used with GFP expressed cells or with other organelles stains for multicolor analysis. It can be used for both suspension and adherent cells and readily adapted for a wide variety of fluorescence platforms.

Platform

Fluorescence microscope

Excitation	Cy3/TRITC filter set
Emission	Cy3/TRITC filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Cy3/TRITC filter set

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoFix™ Red Lysosomal Stain to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	115.774 µL	578.871 µL	1.158 mL	5.789 mL	11.577 mL
5 mM	23.155 µL	115.774 µL	231.548 µL	1.158 mL	2.315 mL
10 mM	11.577 µL	57.887 µL	115.774 µL	578.871 µL	1.158 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	565
Emission (nm)	585

Product Family

Name	Excitation (nm)	Emission (nm)
CytoFix™ Red Mitochondrial Stain	564	651

Images

Figure 1. The fluorescence images of HeLa cells stained with CytoFix™ LysoRed in a 96-well black-wall clear-bottom plate. Image was acquired before (Left) and after (Right) fixation with 4% formaldehyde solution for 20 minutes at RT. The cells were imaged using fluorescence microscope with a Cy3/TRITC filter.