Name: CytoTell® Green
Brand: AAT Bioquest
SKU: 22253
Availability: InStock

CytoTell® Green

CytoTell® Green is a green fluorescent cell proliferation dye designed for long-term tracking of live cells with reduced cytotoxicity and improved performance compared to CFSE.

Reduced cytotoxicity: Exhibits lower cellular toxicity compared to CFSE for improved live cell analysis
Enhanced proliferation tracking: Enables monitoring of successive cell generations through progressive fluorescence dilution
No medium removal required: Does not react with common medium components, simplifying staining workflows
Improved cellular retention: Designed to minimize MDR-mediated dye efflux for long-term cell tracking applications
Broad application compatibility: Suitable for flow cytometry, fluorescence microscopy, intracellular staining workflows, and long-term live cell tracking applications

Cell proliferation assay with CytoTell™ Green and CFSE.  Jurkat cells (~2×10<sup>6</sup> cells/mL) were stained with CytoTell™ Green or CFSE on Day 0. The cells were passed serially at 1:1 ratio on the day specified. Fluorescence intensity was measured with ACEA NovoCyte 3000 flow cytometer in FITC channel on the day of passage. Successive generations were represented by different colors.

Protocol

Catalog	Size	Price	Quantity
22253	500 Tests	Price
22254	2x500 Tests	Price

Overview

CytoTell® Green is a fluorescent cell proliferation indicator developed for monitoring cell division and long-term tracking of live cells by flow cytometry and fluorescence microscopy. As cells divide, the dye is distributed equally between daughter cells, resulting in progressive halving of fluorescence intensity that enables visualization of successive cell generations. CytoTell® Green exhibits peak excitation around 510 nm and can be efficiently excited with the 488 nm blue laser line, making it compatible with standard FITC filter sets, fluorescence microscopes, flow cytometers, and high-content imaging systems.

CytoTell® Green was developed to address several limitations associated with CFSE-based proliferation assays. Compared to CFSE, CytoTell® Green exhibits reduced cytotoxicity, improved intracellular retention, and simplified staining workflows since it does not react significantly with common medium components. The dye can be used for long-term tracking of labeled cells and may be fixed and permeabilized for intracellular target analysis using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTell® Green to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	238.527 µL	1.193 mL	2.385 mL	11.926 mL	23.853 mL
5 mM	47.705 µL	238.527 µL	477.054 µL	2.385 mL	4.771 mL
10 mM	23.853 µL	119.263 µL	238.527 µL	1.193 mL	2.385 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	419.24 g/mol	=	mL	x	mM	=	mmol

Citations

View all 26 citations: Citation Explorer

The CH1 domain influences the expression and antigen sensing of the HIV-specific CH31 IgM-BCR and IgG-BCR

Authors:

Ortiz, Yaneth and Anasti, Kara and Pane, Advaiti K and Cronin, Kenneth and Alam, S Munir and Reth, Michael

Journal:

Proceedings of the National Academy of Sciences (2024): e2404728121

A co-culture system of macrophages with breast cancer tumoroids to study cell interactions and therapeutic responses

Authors:

Raffo-Romero, Antonella and Ziane-Chaouche, Lydia and Salom{\'e}-Desnoulez, Sophie and Hajjaji, Nawale and Fournier, Isabelle and Salzet, Michel and Duhamel, Marie

Journal:

Cell Reports Methods (2024)

Optimizing lentiviral vector formulation conditions for efficient ex vivo transduction of primary human T cells in chimeric antigen receptor T-cell manufacturing

Authors:

Luostarinen, Annu and Kailaanm{\"a}ki, Anssi and Turkki, Vesa and K{\"o}ylij{\"a}rvi, Marjut and K{\"a}yhty, Piia and Leinonen, Hanna and Albers-Skirdenko, Vita and Lipponen, Eevi and Yl{\"a}-Herttuala, Seppo and Kaartinen, Tanja and others,

Journal:

Cytotherapy (2024)

Effective targeting of breast cancer by the inhibition of P-glycoprotein mediated removal of toxic lipid peroxidation byproducts from drug tolerant persister cells

Authors:

Szeb{\'e}nyi, Korn{\'e}lia and F{\"u}redi, Andr{\'a}s and Bajtai, Eszter and Sama, Sai Nagender and Csiszar, Agnes and Gombos, Bal{\'a}zs and Szab{\'o}, P{\'a}l and Grusch, Michael and Szak{\'a}cs, Gergely

Journal:

Drug Resistance Updates (2023): 101007

Concurrent stimulation of monocytes with CSF1 and polarizing cytokines reveals phenotypic and functional differences with classical polarized macrophages

Authors:

An, Liying and Michaeli, Julia and Pallavi, Prama and Breedijk, Annette and Xu, Xin and Dietrich, Nadine and Sigl, Martin and Keese, Michael and Nitschke, Katja and Jarczyk, Jonas and others,

Journal:

Journal of Leukocyte Biology (2022)

Physical properties

Molecular weight	419.24
Solvent	DMSO

Spectral properties

Excitation (nm)	510
Emission (nm)	525

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

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Page updated on May 17, 2026