Name: CytoTell™ Orange
Brand: AAT Bioquest
SKU: 22257
Availability: InStock

CytoTell™ Orange

Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations. Among all the existing fluorescent dyes CFSE is the preferred cell proliferation indicator that is widely used for live cell analysis. However, it is impossible to use CFSE and its fluorescein analogs for GFP-transfected cells or for the applications where a FITC-labeled antibody is used since CFSE and its fluorescein analogs have the excitation and emission spectra almost identical to GFP or FITC. CytoTell™ dyes are well excited at major laser lines such as 405 nm, 488 nm or 633 nm with multicolor emissions. CytoTell™ dyes have minimal cytotoxicity, and are used for the multicolor applications with either GFP cell lines or FITC-labeled antibodies since they have either excitation or emission spectra distinct from fluorescein. CytoTell™ Red is a red fluorescent dye that stains cells evenly. As cells divide, the dye is distributed equally between daughter cells that can be measured as successive halving of the fluorescence intensity of the dye. Up to 8 generations may be visualized. CytoTell™ Orange can also be used for long term tracking of labeled cells. Analysis using two-parameter plots may provide better resolution of each generation, especially between undivided cells and the first generation. Cells labeled with CytoTell™ Orange may be fixed and permeabilized for analysis of intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers. CytoTell™ Orange has a peak excitation of 542 nm and can be excited by the blue (488 nm) and green (532 nm) laser lines. It has a peak emission of ~560 nm and can be detected with a 575/26 band pass filter (equivalent to RPE).

Protocol

COA

Catalog	Size	Price	Quantity
22257	500 Tests	Price
22258	2x500 Tests	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTell™ Orange to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	161.413 µL	807.063 µL	1.614 mL	8.071 mL	16.141 mL
5 mM	32.283 µL	161.413 µL	322.825 µL	1.614 mL	3.228 mL
10 mM	16.141 µL	80.706 µL	161.413 µL	807.063 µL	1.614 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	619.53 g/mol	=	mL	x	mM	=	mmol

Citations

View all 8 citations: Citation Explorer

Inserting EF1$\alpha$-driven CD7-specific CAR at CD7 locus reduces fratricide and enhances tumor rejection

Authors:

Jiang, Jie and Chen, Jiangqing and Liao, Chan and Duan, Yanting and Wang, Yajie and Shang, Kai and Huang, Yanjie and Tang, Yongming and Gao, Xiaofei and Gu, Ying and others,

Journal:

Leukemia (2023): 1--11

In vivo pulse labeling of isochronic cohorts of cells in the central nervous system using FlashTag

Authors:

Govindan, Subashika and Oberst, Polina and Jabaudon, Denis

Journal:

Nature protocols (2018): 2297--2311

CXCL12--CXCR4 Axis Is Required for Contact-Mediated Human B Lymphoid and Plasmacytoid Dendritic Cell Differentiation but Not T Lymphoid Generation

Authors:

Minami, Hirohito and Nagaharu, Keiki and Nakamori, Yoshiki and Ohishi, Kohshi and Shimojo, Naoshi and Kageyama, Yuki and Matsumoto, Takeshi and Sugimoto, Yuka and Tawara, Isao and Masuya, Masahiro and others, undefined

Journal:

The Journal of Immunology (2017): ji1700054

Interaction and Mutual Activation of Different Innate Immune Cells Is Necessary to Kill and Clear Hepatitis C Virus-Infected Cells

Authors:

Klöss, Volker and Grünvogel, Oliver and Wabnitz, Guido and Eigenbrod, Tatjana and Ehrhardt, Stefanie and Lasitschka, Felix and Lohmann, Volker and Dalpke, Alex and er H, undefined

Journal:

Frontiers in Immunology (2017): 1238

Cooperation of innate immune cells during Hepatitis C virus infection

Authors:

Klöss, Volker

Physical properties

Molecular weight	619.53
Solvent	DMSO

Spectral properties

Excitation (nm)	541
Emission (nm)	560

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm or 532 nm laser
Emission	575/26 nm filter
Instrument specification(s)	PE channel

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Page updated on October 13, 2025