Name: CytoTell® Violet 500
Brand: AAT Bioquest
SKU: 22248
Availability: InStock

CytoTell® Violet 500

Flow cytometry combined with fluorescence staining is a powerful tool for analyzing heterogeneous cell populations. CFSE is the preferred cell proliferation indicator widely used for live cell analysis among all the existing fluorescent dyes. However, it is impossible to use CFSE and its fluorescein analogs for GFP-transfected cells or for applications where a FITC-labeled antibody is used since CFSE and its fluorescein analogs have the excitation and emission spectra almost identical to GFP or FITC. CytoTell® dyes are well excited at major laser lines such as 405 nm, 488 nm, or 633 nm with multicolor emissions. CytoTell® dyes have minimal cytotoxicity and are used for multicolor applications with either GFP cell lines or FITC-labeled antibodies since they have either excitation or emission spectra distinct from fluorescein. CytoTell® Violet 500 is a violet laser-excitable green fluorescent dye that stains cells evenly. As cells divide, the dye is distributed equally between daughter cells, which can be measured as a successive halving of the fluorescence intensity of the dye. Cells labeled with CytoTell® Violet 500 may be fixed and permeabilized to analyze intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers. CytoTell® Violet 500 has a peak excitation of 405 nm and can be excited by the violet (405 nm) laser line. It has a peak emission of 500 nm and can be detected with a 500/20 bandpass filter (equivalent to BD Horizon® V500), making it compatible with applications that utilize GFP or FITC antibodies for multicolor cell analysis.

Protocol

COA

Catalog	Size	Price	Quantity
22248	500 Tests	Price
22249	1000 Tests	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTell® Violet 500 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	179.53 µL	897.65 µL	1.795 mL	8.976 mL	17.953 mL
5 mM	35.906 µL	179.53 µL	359.06 µL	1.795 mL	3.591 mL
10 mM	17.953 µL	89.765 µL	179.53 µL	897.65 µL	1.795 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	557.01 g/mol	=	mL	x	mM	=	mmol

Citations

View all 30 citations: Citation Explorer

Cardiomyocyte cell cycle dynamics and proliferation revealed through cardiac-specific transgenesis of fluorescent ubiquitinated cell cycle indicator (FUCCI)

Authors:

Alvarez, R., Jr., Wang, B. J., Quijada, P. J., Avitabile, D., Ho, T., Shaitrit, M., Chavarria, M., Firouzi, F., Ebeid, D., Monsanto, M. M., Navarrete, N., Moshref, M., Siddiqi, S., Broughton, K. M., Bailey, B. A., Gude, N. A., Sussman, M. A.

Journal:

J Mol Cell Cardiol (2019): 154-164

In Vivo Fluorescence Microendoscopic Monitoring of Stent-Induced Fibroblast Cell Proliferation in an Esophageal Mouse Model

Authors:

Jun, E. J., Song, H. Y., Park, J. H., Bae, Y. S., Paulson, B., Lee, S., Cho, Y. C., Tsauo, J., Kim, M. T., Kim, K. Y., Yang, S. G., Kim, J. K.

Journal:

J Vasc Interv Radiol (2018): 1756-1763

Visualizing Breast Cancer Cell Proliferation and Invasion for Assessing Drug Efficacy with a Fluorescent Nanoprobe

Authors:

Luan, M., Yu, L., Li, Y., Pan, W., Gao, X., Wan, X., Li, N., Tang, B.

Journal:

Anal Chem (2017): 10601-10607

Monitoring cell proliferation in vitro with different cellular fluorescent dyes

Authors:

Zolnierowicz, J., Ambrozek-Latecka, M., Kawiak, J., Wasilewska, D., Hoser, G.

Journal:

Folia Histochem Cytobiol (2013): 193-200

A method for evaluating the use of fluorescent dyes to track proliferation in cell lines by dye dilution

Authors:

Begum, J., Day, W., Henderson, C., Purewal, S., Cerveira, J., Summers, H., Rees, P., Davies, D., Filby, A.

Journal:

Cytometry A (2013): 1085-95

Physical properties

Molecular weight	557.01
Solvent	DMSO

Spectral properties

Excitation (nm)	415
Emission (nm)	499

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Flow cytometer
Excitation	405 nm laser
Emission	525/50 nm filter
Instrument specification(s)	Pacific Orange channel

Documents

Protocol
Safety Data Sheet (Catalog 22248)
Safety Data Sheet (Catalog 22249)
Certificate of Analysis

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Page updated on October 21, 2025