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CytoTell™ Violet 500
Flow cytometry combined with fluorescence staining is a powerful tool for analyzing heterogeneous cell populations. CFSE is the preferred cell proliferation indicator widely used for live cell analysis among all the existing fluorescent dyes. However, it is impossible to use CFSE and its fluorescein analogs for GFP-transfected cells or for applications where a FITC-labeled antibody is used since CFSE and its fluorescein analogs have the excitation and emission spectra almost identical to GFP or FITC. CytoTell™ dyes are well excited at major laser lines such as 405 nm, 488 nm, or 633 nm with multicolor emissions. CytoTell™ dyes have minimal cytotoxicity and are used for multicolor applications with either GFP cell lines or FITC-labeled antibodies since they have either excitation or emission spectra distinct from fluorescein. CytoTell™ Violet 500 is a violet laser-excitable green fluorescent dye that stains cells evenly. As cells divide, the dye is distributed equally between daughter cells, which can be measured as a successive halving of the fluorescence intensity of the dye. Cells labeled with CytoTell™ Violet 500 may be fixed and permeabilized to analyze intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers. CytoTell™ Violet 500 has a peak excitation of 405 nm and can be excited by the violet (405 nm) laser line. It has a peak emission of 500 nm and can be detected with a 500/20 bandpass filter (equivalent to BD Horizon® V500), making it compatible with applications that utilize GFP or FITC antibodies for multicolor cell analysis.
Cell proliferation assay with CytoTell&trade;&nbsp;Violet 500.&nbsp; Jurkat cells (~2&times;10<sup>6</sup> cells/mL) were stained with CytoTell&trade; Violet 500 on Day 0. The cells were passed serially at 1:1 ratio on the day specified. Fluorescence intensity was measured with ACEA NovoCyte 3000 flow cytometer with Pacific Orange channel.&nbsp;Successive generations were represented by different colors.
Cell proliferation assay with CytoTell&trade;&nbsp;Violet 500.&nbsp; Jurkat cells (~2&times;10<sup>6</sup> cells/mL) were stained with CytoTell&trade; Violet 500 on Day 0. The cells were passed serially at 1:1 ratio on the day specified. Fluorescence intensity was measured with ACEA NovoCyte 3000 flow cytometer with Pacific Orange channel.&nbsp;Successive generations were represented by different colors.
CatalogSize
Price
Quantity
22248500 Tests
Price
222491000 Tests
Price
 
Physical properties

Molecular weight557.01
SolventDMSO
Spectral properties

Excitation (nm)415
Emission (nm)499
Storage, safety and handling

Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure
Instrument settings

Flow cytometer
Excitation405 nm laser
Emission525/50 nm filter
Instrument specification(s)Pacific Orange channel
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Page updated on October 1, 2025