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CytoTrace™ Orange CMTMR *CAS 323192-14-9*

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Physical properties
Molecular weight554.04
SolventDMSO
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
323192-14-9
Molecular weight
554.04
CytoTrace™ Orange CMTMR is chemically same to CellTracker™ Orange CMTMR. CytoTrace™ Orange CMTMR freely passes through cell membranes into cells, where it is transformed into cell membrane-impermeant reaction products. CytoTrace™ Orange CMTMR dye is retained in living cells through several generations. The dye is transferred to daughter cells, but not adjacent cells in a population. CytoTrace™ Orange CMTMR dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties. CMTMR dye is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CytoTrace™ Orange CMTMR dye are well separated from GFP (green fluorescent protein) and spectra allowing for multiplexing.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ Orange CMTMR *CAS 323192-14-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM180.492 µL902.462 µL1.805 mL9.025 mL18.049 mL
5 mM36.098 µL180.492 µL360.985 µL1.805 mL3.61 mL
10 mM18.049 µL90.246 µL180.492 µL902.462 µL1.805 mL

Molarity calculator

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References


View all 23 references: Citation Explorer
Fluorescence-Based Transport Assays Revisited in a Human Renal Proximal Tubule Cell Line
Authors: Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.
Journal: Mol Pharm (2016): 933
The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance
Authors: Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.
Journal: Phytomedicine (2015): 713
Cell membrane tracker based on restriction of intramolecular rotation
Authors: Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.
Journal: ACS Appl Mater Interfaces (2014): 8971
Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration
Authors: Beem E, Segal MS.
Journal: J Fluoresc (2013): 975
A multiple model probability hypothesis density tracker for time-lapse cell microscopy sequences
Authors: Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.
Journal: Inf Process Med Imaging (2013): 110
TLM-Tracker: software for cell segmentation, tracking and lineage analysis in time-lapse microscopy movies
Authors: Klein J, Leupold S, Biegler I, Biedendieck R, Munch R, Jahn D.
Journal: Bioinformatics (2012): 2276
The interplay between Leishmania promastigotes and human Natural Killer cells in vitro leads to direct lysis of Leishmania by NK cells and modulation of NK cell activity by Leishmania promastigotes
Authors: Lieke T, Nylen S, Eidsmo L, Schmetz C, Berg L, Akuffo H.
Journal: Parasitology (2011): 1898
The exocytosis of fluorescent nanodiamond and its use as a long-term cell tracker
Authors: Fang CY, Vaijayanthimala V, Cheng CA, Yeh SH, Chang CF, Li CL, Chang HC.
Journal: Small (2011): 3363
Horizontal DNA transfer from donor to host cells as an alternative mechanism of epithelial chimerism after allogeneic hematopoietic cell transplantation
Authors: Waterhouse M, Themeli M, Bertz H, Zoumbos N, Finke J, Spyridonidis A.
Journal: Biol Blood Marrow Transplant (2011): 319
Overlap-Based Cell Tracker
Authors: Chalfoun J, Cardone A, Dima AA, Allen DP, Halter MW.
Journal: J Res Natl Inst Stand Technol (2010): 477