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CytoTrace™ Orange CMTMR *CAS 323192-14-9*

CytoTrace™ Orange CMTMR is chemically same to CellTracker™ Orange CMTMR. CytoTrace™ Orange CMTMR freely passes through cell membranes into cells, where it is transformed into cell membrane-impermeant reaction products. CytoTrace™ Orange CMTMR dye is retained in living cells through several generations. The dye is transferred to daughter cells, but not adjacent cells in a population. CytoTrace™ Orange CMTMR dye is designed to display fluorescence for at least 72 hours, and the dye exhibits ideal tracking properties. CMTMR dye is stable, nontoxic at working concentrations, well retained in cells, and brightly fluorescent at physiological pH. Additionally, the excitation and emission spectra of CytoTrace™ Orange CMTMR dye are well separated from GFP (green fluorescent protein) and spectra allowing for multiplexing.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ Orange CMTMR *CAS 323192-14-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM180.492 µL902.462 µL1.805 mL9.025 mL18.049 mL
5 mM36.098 µL180.492 µL360.985 µL1.805 mL3.61 mL
10 mM18.049 µL90.246 µL180.492 µL902.462 µL1.805 mL

Molarity calculator

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References

View all 23 references: Citation Explorer
Fluorescence-Based Transport Assays Revisited in a Human Renal Proximal Tubule Cell Line
Authors: Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.
Journal: Mol Pharm (2016): 933
The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance
Authors: Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.
Journal: Phytomedicine (2015): 713
Cell membrane tracker based on restriction of intramolecular rotation
Authors: Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.
Journal: ACS Appl Mater Interfaces (2014): 8971
Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration
Authors: Beem E, Segal MS.
Journal: J Fluoresc (2013): 975
A multiple model probability hypothesis density tracker for time-lapse cell microscopy sequences
Authors: Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.
Journal: Inf Process Med Imaging (2013): 110
Page updated on December 1, 2024

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Physical properties

Molecular weight

554.04

Solvent

DMSO

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

323192-14-9
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