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CytoTrace™ Red CFDA

Image of HeLa cells in a 96-well Costar black wall/clear bottom plate stained with CytoTrace™ Red CFDA (TRITC filter).
Image of HeLa cells in a 96-well Costar black wall/clear bottom plate stained with CytoTrace™ Red CFDA (TRITC filter).
Ordering information
Price ()
Catalog Number22016
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight652.43
SolventDMSO
Spectral properties
Excitation (nm)562
Emission (nm)576
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
652.43
Excitation (nm)
562
Emission (nm)
576
CytoTrace™ Red fluorescent probe retains the spectral properties of Cy3/TRITC, thus its signal can be easily monitored using fluorescence microscopy or flow cytometry. It freely passes through cell membranes and is converted to cell-impermeant products upon reactions with cellular components. The cell-impermeant reaction product is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CytoTrace™ Red probe is typically fluorescent and viable for at least 24 hours, making this probe an excellent long-term cell tracer. The staining pattern can be fixed with formaldehyde or glutaraldehyde for signal amplification and other applications. Its spectra are well separated from those of GFP or FITC-labeled antibodies, making it an excellent choice for multiplexing with GFP cell lines or FITC-labeled antibodies.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ Red CFDA to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM153.273 µL766.366 µL1.533 mL7.664 mL15.327 mL
5 mM30.655 µL153.273 µL306.546 µL1.533 mL3.065 mL
10 mM15.327 µL76.637 µL153.273 µL766.366 µL1.533 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Excitation (nm)562
Emission (nm)576

Citations


View all 1 citations: Citation Explorer
The increased ratio of Mg2+/Ca2+ from degrading magnesium alloys directs macrophage fate for functionalized growth of endothelial cells
Authors: Hou, Ya-chen and Witte, Frank and Li, Jingan and Guan, Shaokang
Journal: Smart Materials in Medicine (2022)

References


View all 23 references: Citation Explorer
Fluorescence-Based Transport Assays Revisited in a Human Renal Proximal Tubule Cell Line
Authors: Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.
Journal: Mol Pharm (2016): 933
The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance
Authors: Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.
Journal: Phytomedicine (2015): 713
Cell membrane tracker based on restriction of intramolecular rotation
Authors: Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.
Journal: ACS Appl Mater Interfaces (2014): 8971
Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration
Authors: Beem E, Segal MS.
Journal: J Fluoresc (2013): 975
A multiple model probability hypothesis density tracker for time-lapse cell microscopy sequences
Authors: Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.
Journal: Inf Process Med Imaging (2013): 110
TLM-Tracker: software for cell segmentation, tracking and lineage analysis in time-lapse microscopy movies
Authors: Klein J, Leupold S, Biegler I, Biedendieck R, Munch R, Jahn D.
Journal: Bioinformatics (2012): 2276
The interplay between Leishmania promastigotes and human Natural Killer cells in vitro leads to direct lysis of Leishmania by NK cells and modulation of NK cell activity by Leishmania promastigotes
Authors: Lieke T, Nylen S, Eidsmo L, Schmetz C, Berg L, Akuffo H.
Journal: Parasitology (2011): 1898
The exocytosis of fluorescent nanodiamond and its use as a long-term cell tracker
Authors: Fang CY, Vaijayanthimala V, Cheng CA, Yeh SH, Chang CF, Li CL, Chang HC.
Journal: Small (2011): 3363
Horizontal DNA transfer from donor to host cells as an alternative mechanism of epithelial chimerism after allogeneic hematopoietic cell transplantation
Authors: Waterhouse M, Themeli M, Bertz H, Zoumbos N, Finke J, Spyridonidis A.
Journal: Biol Blood Marrow Transplant (2011): 319
Overlap-Based Cell Tracker
Authors: Chalfoun J, Cardone A, Dima AA, Allen DP, Halter MW.
Journal: J Res Natl Inst Stand Technol (2010): 477