Name: CytoTrace™ Red CFDA
Brand: AAT Bioquest
SKU: 22016
Price: 232 USD
Availability: InStock

CytoTrace™ Red CFDA

CytoTrace™ Red fluorescent probe retains the spectral properties of Cy3/TRITC, thus its signal can be easily monitored using fluorescence microscopy or flow cytometry. It freely passes through cell membranes and is converted to cell-impermeant products upon reactions with cellular components. The cell-impermeant reaction product is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CytoTrace™ Red probe is typically fluorescent and viable for at least 24 hours, making this probe an excellent long-term cell tracer. The staining pattern can be fixed with formaldehyde or glutaraldehyde for signal amplification and other applications. Its spectra are well separated from those of GFP or FITC-labeled antibodies, making it an excellent choice for multiplexing with GFP cell lines or FITC-labeled antibodies.

Protocol

SDS

Catalog	Size	Price	Quantity
22016	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ Red CFDA to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	153.273 µL	766.366 µL	1.533 mL	7.664 mL	15.327 mL
5 mM	30.655 µL	153.273 µL	306.546 µL	1.533 mL	3.065 mL
10 mM	15.327 µL	76.637 µL	153.273 µL	766.366 µL	1.533 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	652.43 g/mol	=	mL	x	mM	=	mmol

Citations

View all 3 citations: Citation Explorer

Exploring the Functions of Mutant p53 through TP53 Knockout in HaCaT Keratinocytes

Authors:

Romashin, Daniil and Rusanov, Alexander and Arzumanian, Viktoriia and Varshaver, Alexandra and Poverennaya, Ekaterina and Vakhrushev, Igor and Netrusov, Alexander and Luzgina, Nataliya

Journal:

Current Issues in Molecular Biology (2024): 1451--1466

Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application

Authors:

Hou, Ya-chen and Li, Jing-an and Cao, Chang and Su, Chang and Qin, Zhen and Zhang, Ge and Guo, Jia-cheng and Tang, Jun-nan and Zhang, Jin-ying and Guan, Shao-kang

Journal:

Journal of Magnesium and Alloys (2023)

The increased ratio of Mg2+/Ca2+ from degrading magnesium alloys directs macrophage fate for functionalized growth of endothelial cells

Authors:

Hou, Ya-chen and Witte, Frank and Li, Jingan and Guan, Shaokang

Journal:

Smart Materials in Medicine (2022)

References

View all 23 references: Citation Explorer

Fluorescence-Based Transport Assays Revisited in a Human Renal Proximal Tubule Cell Line

Authors:

Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.

Journal:

Mol Pharm (2016): 933

The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance

Authors:

Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.

Journal:

Phytomedicine (2015): 713

Cell membrane tracker based on restriction of intramolecular rotation

Authors:

Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.

Journal:

ACS Appl Mater Interfaces (2014): 8971

Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration

Authors:

Beem E, Segal MS.

Journal:

J Fluoresc (2013): 975

A multiple model probability hypothesis density tracker for time-lapse cell microscopy sequences

Authors:

Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.

Journal:

Inf Process Med Imaging (2013): 110

Physical properties

Molecular weight	652.43
Solvent	DMSO

Spectral properties

Excitation (nm)	562
Emission (nm)	576

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Brochure: Cell Viability & Proliferation

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Page updated on November 13, 2025