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CytoTrace™ Red CFDA

Image of HeLa cells in a 96-well Costar black wall/clear bottom plate stained with CytoTrace™ Red CFDA (TRITC filter).
Image of HeLa cells in a 96-well Costar black wall/clear bottom plate stained with CytoTrace™ Red CFDA (TRITC filter).
Image of HeLa cells in a 96-well Costar black wall/clear bottom plate stained with CytoTrace™ Red CFDA (TRITC filter).
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Physical properties
Molecular weight652.43
Spectral properties
Excitation (nm)562
Emission (nm)576
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
CytoTrace™ Red fluorescent probe retains the spectral properties of Cy3/TRITC, thus its signal can be easily monitored using fluorescence microscopy or flow cytometry. It freely passes through cell membranes and is converted to cell-impermeant products upon reactions with cellular components. The cell-impermeant reaction product is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CytoTrace™ Red probe is typically fluorescent and viable for at least 24 hours, making this probe an excellent long-term cell tracer. The staining pattern can be fixed with formaldehyde or glutaraldehyde for signal amplification and other applications. Its spectra are well separated from those of GFP or FITC-labeled antibodies, making it an excellent choice for multiplexing with GFP cell lines or FITC-labeled antibodies.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ Red CFDA to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM153.273 µL766.366 µL1.533 mL7.664 mL15.327 mL
5 mM30.655 µL153.273 µL306.546 µL1.533 mL3.065 mL
10 mM15.327 µL76.637 µL153.273 µL766.366 µL1.533 mL

Molarity calculator

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Spectral properties

Excitation (nm)562
Emission (nm)576



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