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CytoTrace™ Red CFDA

CytoTrace™ Red fluorescent probe retains the spectral properties of Cy3/TRITC, thus its signal can be easily monitored using fluorescence microscopy or flow cytometry. It freely passes through cell membranes and is converted to cell-impermeant products upon reactions with cellular components. The cell-impermeant reaction product is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with CytoTrace™ Red probe is typically fluorescent and viable for at least 24 hours, making this probe an excellent long-term cell tracer. The staining pattern can be fixed with formaldehyde or glutaraldehyde for signal amplification and other applications. Its spectra are well separated from those of GFP or FITC-labeled antibodies, making it an excellent choice for multiplexing with GFP cell lines or FITC-labeled antibodies.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ Red CFDA to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM153.273 µL766.366 µL1.533 mL7.664 mL15.327 mL
5 mM30.655 µL153.273 µL306.546 µL1.533 mL3.065 mL
10 mM15.327 µL76.637 µL153.273 µL766.366 µL1.533 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum

Citations

View all 3 citations: Citation Explorer
Exploring the Functions of Mutant p53 through TP53 Knockout in HaCaT Keratinocytes
Authors: Romashin, Daniil and Rusanov, Alexander and Arzumanian, Viktoriia and Varshaver, Alexandra and Poverennaya, Ekaterina and Vakhrushev, Igor and Netrusov, Alexander and Luzgina, Nataliya
Journal: Current Issues in Molecular Biology (2024): 1451--1466
Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application
Authors: Hou, Ya-chen and Li, Jing-an and Cao, Chang and Su, Chang and Qin, Zhen and Zhang, Ge and Guo, Jia-cheng and Tang, Jun-nan and Zhang, Jin-ying and Guan, Shao-kang
Journal: Journal of Magnesium and Alloys (2023)
The increased ratio of Mg2+/Ca2+ from degrading magnesium alloys directs macrophage fate for functionalized growth of endothelial cells
Authors: Hou, Ya-chen and Witte, Frank and Li, Jingan and Guan, Shaokang
Journal: Smart Materials in Medicine (2022)

References

View all 23 references: Citation Explorer
Fluorescence-Based Transport Assays Revisited in a Human Renal Proximal Tubule Cell Line
Authors: Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.
Journal: Mol Pharm (2016): 933
The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance
Authors: Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.
Journal: Phytomedicine (2015): 713
Cell membrane tracker based on restriction of intramolecular rotation
Authors: Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.
Journal: ACS Appl Mater Interfaces (2014): 8971
Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration
Authors: Beem E, Segal MS.
Journal: J Fluoresc (2013): 975
A multiple model probability hypothesis density tracker for time-lapse cell microscopy sequences
Authors: Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.
Journal: Inf Process Med Imaging (2013): 110
Page updated on November 3, 2024

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Catalog Number22016
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Physical properties

Molecular weight

652.43

Solvent

DMSO

Spectral properties

Excitation (nm)

562

Emission (nm)

576

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Image of HeLa cells in a 96-well Costar black wall/clear bottom plate stained with CytoTrace&trade; Red CFDA (TRITC filter).
Image of HeLa cells in a 96-well Costar black wall/clear bottom plate stained with CytoTrace&trade; Red CFDA (TRITC filter).
Image of HeLa cells in a 96-well Costar black wall/clear bottom plate stained with CytoTrace&trade; Red CFDA (TRITC filter).