Name: CytoTrace™ Ultra Green
Brand: AAT Bioquest
SKU: 21800
Price: 290 USD
Availability: InStock

CytoTrace™ Ultra Green

CMFDA (5-chloromethylfluorescein diacetate) has been used as a prominent cell tracker in the research community for a long time. We are introducing a new generation of fluorescent dye, named CytoTrace™ Ultra Green that is well suited for tracking the cell movement or location. The dye is well retained in cells, allowing tracking live cells for quite a few generations. Compared to CMFDA under the same conditions, CytoTrace™ Ultra Green is significantly brighter, more photostable and robust to use. Its fluorescence signal is also fixable. The signal generated by this dye is highly stable after fixation, making it ideal candidate to combine with other types of assays such as cytotoxicity etc for multicolor applications. The excitation and emission spectra of CytoTrace™ Ultra Green is identical to those of FITC, and are well separated from the common red fluorescent dyes such as Texas Red, Cy5, Cy7, iFluor® 647 & 750, Alexa Fluor® 647 and 750. CytoTrace™ Ultra Green can be readily used for tracking live cells for various biological applications, and compatible with flow cytometry and fluorescence microscopy.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21800	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ Ultra Green to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	121.548 µL	607.74 µL	1.215 mL	6.077 mL	12.155 mL
5 mM	24.31 µL	121.548 µL	243.096 µL	1.215 mL	2.431 mL
10 mM	12.155 µL	60.774 µL	121.548 µL	607.74 µL	1.215 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	822.72 g/mol	=	mL	x	mM	=	mmol

Citations

View all 5 citations: Citation Explorer

SPF-CellTracker: Tracking Multiple Cells with Strongly-Correlated Moves Using a Spatial Particle Filter

Authors:

Hirose, O., Kawaguchi, S., Tokunaga, T., Toyoshima, Y., Teramoto, T., Kuge, S., Ishihara, T., Iino, Y., Yoshida, R.

Journal:

IEEE/ACM Trans Comput Biol Bioinform (2018): 1822-1831

Challenges in using CellTracker Green on foraminifers that host algal endosymbionts

Authors:

Ross, B. J., Hallock, P.

Journal:

PeerJ (2018): e5304

CellTracker (not only) for dummies

Authors:

Piccinini, F., Kiss, A., Horvath, P.

Journal:

Bioinformatics (2016): 955-7

In vitro evaluation of the effects of cysticidal drugs in the Taenia crassiceps cysticerci ORF strain using the fluorescent CellTracker CMFDA

Authors:

Trejo-Chavez, H., Garcia-Vilchis, D., Reynoso-Ducoing, O., Ambrosio, J. R.

Journal:

Exp Parasitol (2011): 294-9

Survival of benthic foraminifera under hypoxic conditions: results of an experimental study using the CellTracker Green method

Authors:

Pucci, F., Geslin, E., Barras, C., Morigi, C., Sabbatini, A., Negri, A., Jorissen, F. J.

Journal:

Mar Pollut Bull (2009): 336-51

References

View all 23 references: Citation Explorer

Fluorescence-Based Transport Assays Revisited in a Human Renal Proximal Tubule Cell Line

Authors:

Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.

Journal:

Mol Pharm (2016): 933

The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance

Authors:

Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.

Journal:

Phytomedicine (2015): 713

Cell membrane tracker based on restriction of intramolecular rotation

Authors:

Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.

Journal:

ACS Appl Mater Interfaces (2014): 8971

Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration

Authors:

Beem E, Segal MS.

Journal:

J Fluoresc (2013): 975

A multiple model probability hypothesis density tracker for time-lapse cell microscopy sequences

Authors:

Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.

Journal:

Inf Process Med Imaging (2013): 110

Physical properties

Molecular weight	822.72
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 22, 2025