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CytoTrace™ Ultra Green
CMFDA (5-chloromethylfluorescein diacetate) has been used as a prominent cell tracker in the research community for a long time. We are introducing a new generation of fluorescent dye, named CytoTrace™ Ultra Green that is well suited for tracking the cell movement or location. The dye is well retained in cells, allowing tracking live cells for quite a few generations. Compared to CMFDA under the same conditions, CytoTrace™ Ultra Green is significantly brighter, more photostable and robust to use. Its fluorescence signal is also fixable. The signal generated by this dye is highly stable after fixation, making it ideal candidate to combine with other types of assays such as cytotoxicity etc for multicolor applications. The excitation and emission spectra of CytoTrace™ Ultra Green is identical to those of FITC, and are well separated from the common red fluorescent dyes such as Texas Red, Cy5, Cy7, iFluor® 647 & 750, Alexa Fluor® 647 and 750. CytoTrace™ Ultra Green can be readily used for tracking live cells for various biological applications, and compatible with flow cytometry and fluorescence microscopy.
The comparison in the fluorescence intensity of CytoTrace&trade; Ultra Green with CMFDA in Jurkat cells. Jurkat cells were dye loaded with CytoTrace&trade; Ultra Green or CMFDA for 30 minutes in a 37 <sup>o</sup>C, 5% CO<sub>2</sub> incubator. The fluorescence intensity was measured using ACEA NovoCyte 3000 flow cytometer with FITC channel.
The comparison in the fluorescence intensity of CytoTrace&trade; Ultra Green with CMFDA in Jurkat cells. Jurkat cells were dye loaded with CytoTrace&trade; Ultra Green or CMFDA for 30 minutes in a 37 <sup>o</sup>C, 5% CO<sub>2</sub> incubator. The fluorescence intensity was measured using ACEA NovoCyte 3000 flow cytometer with FITC channel.
CatalogSize
Price
Quantity
218001 mg
Price
 
Physical properties

Molecular weight822.72
SolventDMSO
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 13, 2025