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CytoTrace™ Green CMFDA

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Physical properties
Molecular weight464.86
Spectral properties
Excitation (nm)498
Emission (nm)517
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
CytoTrace™ Green CMFDA is an excellent replacement to CellTracker™ Green CMFDA (Invitrogen C2925, CellTracker™ is the trademark of Invitrogen). CMFDA fluorescent probe retains the spectral properties of FITC, thus its signal can be typically monitored using fluorescence microscopy or flow cytometry. It freely passes through cell membranes and is converted to cell-impermeant products upon reactions with cellular components. The cell-impermeant reaction product is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with Cytotrace™ Green probe is typically fluorescent and viable for at least 24 hours, making this probe an excellent long-term cell tracer. The staining pattern can be fixed with formaldehyde or glutaraldehyde for signal amplification and other applications.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ Green CMFDA to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM215.119 µL1.076 mL2.151 mL10.756 mL21.512 mL
5 mM43.024 µL215.119 µL430.237 µL2.151 mL4.302 mL
10 mM21.512 µL107.559 µL215.119 µL1.076 mL2.151 mL

Molarity calculator

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Spectral properties

Excitation (nm)498
Emission (nm)517



View all 8 citations: Citation Explorer
STIM1-Dependent Store-Operated Calcium Entry Mediates Sex Differences in Macrophage Chemotaxis and Monocyte Recruitment.
Authors: Fresquez, Adriana M and Hogan, James O and Rivera, Patricia and Patterson, Kristen M and Singer, Kanakadurga and Reynolds, Joseph M and White, Carl
Journal: Journal of Biological Chemistry (2024): 107422
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Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application
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An integrated strategy combining network toxicology and feature-based molecular networking for exploring hepatotoxic constituents and mechanism of Epimedii Folium-induced hepatotoxicity in vitro
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Journal: Food and Chemical Toxicology (2023): 113785
A promoting nitric oxide-releasing coating containing copper ion on ZE21B alloy for potential vascular stent application
Authors: Jia, Qianying and Jia, Qinggong and Zhu, Shijie and Zheng, Yufeng and Mine, Yoji and Takashima, Kazuki and Guan, Shaokang
Journal: Journal of Magnesium and Alloys (2022)
The increased ratio of Mg2+/Ca2+ from degrading magnesium alloys directs macrophage fate for functionalized growth of endothelial cells
Authors: Hou, Ya-chen and Witte, Frank and Li, Jingan and Guan, Shaokang
Journal: Smart Materials in Medicine (2022)
Clinical Strains of Helicobacter pylori With Strong Cell Invasiveness and the Protective Effect of Patchouli Alcohol by Improving miR-30b/C Mediated Xenophagy
Authors: Xu, Yifei and Deng, Qiuhua and Zhong, Yuanzun and Jing, Li and Li, Haiwen and Li, Jingwei and Yu, Huimin and Pan, Huafeng and Guo, Shaoju and Cao, Hongying and others,
Journal: Frontiers in Pharmacology (2021): 1037
Inflation-collapse dynamics drive patterning and morphogenesis in intestinal organoids
Authors: Tallapragada, Naren P and Cambra, Hailey M and Wald, Tomas and Jalbert, Samantha Keough and Abraham, Diana M and Klein, Ophir D and Klein, Allon M
Journal: Cell Stem Cell (2021)


View all 23 references: Citation Explorer
Fluorescence-Based Transport Assays Revisited in a Human Renal Proximal Tubule Cell Line
Authors: Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.
Journal: Mol Pharm (2016): 933
The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance
Authors: Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.
Journal: Phytomedicine (2015): 713
Cell membrane tracker based on restriction of intramolecular rotation
Authors: Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.
Journal: ACS Appl Mater Interfaces (2014): 8971
Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration
Authors: Beem E, Segal MS.
Journal: J Fluoresc (2013): 975
A multiple model probability hypothesis density tracker for time-lapse cell microscopy sequences
Authors: Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.
Journal: Inf Process Med Imaging (2013): 110
TLM-Tracker: software for cell segmentation, tracking and lineage analysis in time-lapse microscopy movies
Authors: Klein J, Leupold S, Biegler I, Biedendieck R, Munch R, Jahn D.
Journal: Bioinformatics (2012): 2276
The exocytosis of fluorescent nanodiamond and its use as a long-term cell tracker
Authors: Fang CY, Vaijayanthimala V, Cheng CA, Yeh SH, Chang CF, Li CL, Chang HC.
Journal: Small (2011): 3363
The interplay between Leishmania promastigotes and human Natural Killer cells in vitro leads to direct lysis of Leishmania by NK cells and modulation of NK cell activity by Leishmania promastigotes
Authors: Lieke T, Nylen S, Eidsmo L, Schmetz C, Berg L, Akuffo H.
Journal: Parasitology (2011): 1898
Horizontal DNA transfer from donor to host cells as an alternative mechanism of epithelial chimerism after allogeneic hematopoietic cell transplantation
Authors: Waterhouse M, Themeli M, Bertz H, Zoumbos N, Finke J, Spyridonidis A.
Journal: Biol Blood Marrow Transplant (2011): 319
Overlap-Based Cell Tracker
Authors: Chalfoun J, Cardone A, Dima AA, Allen DP, Halter MW.
Journal: J Res Natl Inst Stand Technol (2010): 477