Name: CytoTrace™ Green CMFDA
Brand: AAT Bioquest
SKU: 22017
Price: 232 USD
Availability: InStock

CytoTrace™ Green CMFDA

CytoTrace™ Green CMFDA is an excellent replacement to CellTracker™ Green CMFDA (Invitrogen C2925, CellTracker™ is the trademark of Invitrogen). CMFDA fluorescent probe retains the spectral properties of FITC, thus its signal can be typically monitored using fluorescence microscopy or flow cytometry. It freely passes through cell membranes and is converted to cell-impermeant products upon reactions with cellular components. The cell-impermeant reaction product is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with Cytotrace™ Green probe is typically fluorescent and viable for at least 24 hours, making this probe an excellent long-term cell tracer. The staining pattern can be fixed with formaldehyde or glutaraldehyde for signal amplification and other applications.

Protocol

SDS

Catalog	Size	Price	Quantity
22017	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ Green CMFDA to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	215.119 µL	1.076 mL	2.151 mL	10.756 mL	21.512 mL
5 mM	43.024 µL	215.119 µL	430.237 µL	2.151 mL	4.302 mL
10 mM	21.512 µL	107.559 µL	215.119 µL	1.076 mL	2.151 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	464.86 g/mol	=	mL	x	mM	=	mmol

Citations

View all 11 citations: Citation Explorer

Epstein--Barr virus induces aberrant B cell migration and diapedesis via FAK-dependent chemotaxis pathways

Authors:

Delecluse, Susanne and Baccianti, Francesco and Zala, Manon and Steffens, Alina and Drenda, Carolin and Judt, Daniel and Holland-Letz, Tim and Poirey, Remy and Sujobert, Pierre and Delecluse, Henri-Jacques

Journal:

Nature Communications (2025): 1--16

Understanding transport mechanisms across the human blood-brain barrier in Alzheimer's disease

Authors:

Bell, Luisa

Epstein-Barr virus induces B cell migration and diapedesis

Authors:

Delecluse, Henri-Jacques and Delecluse, Susanne and Baccianti, Francesco and Steffens, Alina and Holland-Letz, Tim and Drenda, Carolin and Sujobert, Pierre and others,

STIM1-Dependent Store-Operated Calcium Entry Mediates Sex Differences in Macrophage Chemotaxis and Monocyte Recruitment.

Authors:

Fresquez, Adriana M and Hogan, James O and Rivera, Patricia and Patterson, Kristen M and Singer, Kanakadurga and Reynolds, Joseph M and White, Carl

Journal:

Journal of Biological Chemistry (2024): 107422

A Cu (Ⅱ)-Eluting Coating through Silk fibroin film on ZE21B alloy designed for in situ endotheliazation biofunction

Authors:

Jia, Qianying and Jia, Qinggong and Zhu, Shijie and Zheng, Yufeng and Guan, Shaokang

Journal:

Colloids and Surfaces B: Biointerfaces (2024): 113808

References

View all 23 references: Citation Explorer

Fluorescence-Based Transport Assays Revisited in a Human Renal Proximal Tubule Cell Line

Authors:

Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.

Journal:

Mol Pharm (2016): 933

The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance

Authors:

Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.

Journal:

Phytomedicine (2015): 713

Cell membrane tracker based on restriction of intramolecular rotation

Authors:

Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.

Journal:

ACS Appl Mater Interfaces (2014): 8971

Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration

Authors:

Beem E, Segal MS.

Journal:

J Fluoresc (2013): 975

A multiple model probability hypothesis density tracker for time-lapse cell microscopy sequences

Authors:

Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.

Journal:

Inf Process Med Imaging (2013): 110

Physical properties

Molecular weight	464.86
Solvent	DMSO

Spectral properties

Excitation (nm)	498
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	136832-63-8

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis
Brochure: Cell Apoptosis & Proliferation
Brochure: Cell Viability & Proliferation

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Page updated on October 31, 2025