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CytoTrace™ Green CMFDA
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Application notes
CytoTrace™ Green CMFDA is an excellent replacement to CellTracker™ Green CMFDA (Invitrogen C2925, CellTracker™ is the trademark of Invitrogen). CMFDA fluorescent probe retains the spectral properties of FITC, thus its signal can be typically monitored using fluorescence microscopy or flow cytometry. It freely passes through cell membranes and is converted to cell-impermeant products upon reactions with cellular components. The cell-impermeant reaction product is passed to daughter cells through several generations but is not transferred to adjacent cells in the population. Cells that are loaded with Cytotrace™ Green probe is typically fluorescent and viable for at least 24 hours, making this probe an excellent long-term cell tracer. The staining pattern can be fixed with formaldehyde or glutaraldehyde for signal amplification and other applications.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of CytoTrace™ Green CMFDA to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 215.119 µL | 1.076 mL | 2.151 mL | 10.756 mL | 21.512 mL |
| 5 mM | 43.024 µL | 215.119 µL | 430.237 µL | 2.151 mL | 4.302 mL |
| 10 mM | 21.512 µL | 107.559 µL | 215.119 µL | 1.076 mL | 2.151 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Citations
View all 8 citations: Citation Explorer
STIM1-Dependent Store-Operated Calcium Entry Mediates Sex Differences in Macrophage Chemotaxis and Monocyte Recruitment.
Authors: Fresquez, Adriana M and Hogan, James O and Rivera, Patricia and Patterson, Kristen M and Singer, Kanakadurga and Reynolds, Joseph M and White, Carl
Journal: Journal of Biological Chemistry (2024): 107422
Authors: Fresquez, Adriana M and Hogan, James O and Rivera, Patricia and Patterson, Kristen M and Singer, Kanakadurga and Reynolds, Joseph M and White, Carl
Journal: Journal of Biological Chemistry (2024): 107422
A Cu (Ⅱ)-Eluting Coating through Silk fibroin film on ZE21B alloy designed for in situ endotheliazation biofunction
Authors: Jia, Qianying and Jia, Qinggong and Zhu, Shijie and Zheng, Yufeng and Guan, Shaokang
Journal: Colloids and Surfaces B: Biointerfaces (2024): 113808
Authors: Jia, Qianying and Jia, Qinggong and Zhu, Shijie and Zheng, Yufeng and Guan, Shaokang
Journal: Colloids and Surfaces B: Biointerfaces (2024): 113808
Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application
Authors: Hou, Ya-chen and Li, Jing-an and Cao, Chang and Su, Chang and Qin, Zhen and Zhang, Ge and Guo, Jia-cheng and Tang, Jun-nan and Zhang, Jin-ying and Guan, Shao-kang
Journal: Journal of Magnesium and Alloys (2023)
Authors: Hou, Ya-chen and Li, Jing-an and Cao, Chang and Su, Chang and Qin, Zhen and Zhang, Ge and Guo, Jia-cheng and Tang, Jun-nan and Zhang, Jin-ying and Guan, Shao-kang
Journal: Journal of Magnesium and Alloys (2023)
An integrated strategy combining network toxicology and feature-based molecular networking for exploring hepatotoxic constituents and mechanism of Epimedii Folium-induced hepatotoxicity in vitro
Authors: Zhao, Zhen and Du, Jin-Fa and Wang, Qiao-Lei and Qiu, Fang-Ning and Chen, Xu-Yan and Liu, Feng-Jie and Li, Ping and Jiang, Yan and Li, Hui-Jun
Journal: Food and Chemical Toxicology (2023): 113785
Authors: Zhao, Zhen and Du, Jin-Fa and Wang, Qiao-Lei and Qiu, Fang-Ning and Chen, Xu-Yan and Liu, Feng-Jie and Li, Ping and Jiang, Yan and Li, Hui-Jun
Journal: Food and Chemical Toxicology (2023): 113785
A promoting nitric oxide-releasing coating containing copper ion on ZE21B alloy for potential vascular stent application
Authors: Jia, Qianying and Jia, Qinggong and Zhu, Shijie and Zheng, Yufeng and Mine, Yoji and Takashima, Kazuki and Guan, Shaokang
Journal: Journal of Magnesium and Alloys (2022)
Authors: Jia, Qianying and Jia, Qinggong and Zhu, Shijie and Zheng, Yufeng and Mine, Yoji and Takashima, Kazuki and Guan, Shaokang
Journal: Journal of Magnesium and Alloys (2022)
References
View all 23 references: Citation Explorer
Fluorescence-Based Transport Assays Revisited in a Human Renal Proximal Tubule Cell Line
Authors: Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.
Journal: Mol Pharm (2016): 933
Authors: Caetano-Pinto P, Janssen MJ, Gijzen L, Verscheijden L, Wilmer MJ, Masereeuw R.
Journal: Mol Pharm (2016): 933
The variable chemotherapeutic response of Malabaricone-A in leukemic and solid tumor cell lines depends on the degree of redox imbalance
Authors: Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.
Journal: Phytomedicine (2015): 713
Authors: Manna A, De Sarkar S, De S, Bauri AK, Chattopadhyay S, Chatterjee M.
Journal: Phytomedicine (2015): 713
Cell membrane tracker based on restriction of intramolecular rotation
Authors: Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.
Journal: ACS Appl Mater Interfaces (2014): 8971
Authors: Zhang C, Jin S, Yang K, Xue X, Li Z, Jiang Y, Chen WQ, Dai L, Zou G, Liang XJ.
Journal: ACS Appl Mater Interfaces (2014): 8971
Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration
Authors: Beem E, Segal MS.
Journal: J Fluoresc (2013): 975
Authors: Beem E, Segal MS.
Journal: J Fluoresc (2013): 975
A multiple model probability hypothesis density tracker for time-lapse cell microscopy sequences
Authors: Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.
Journal: Inf Process Med Imaging (2013): 110
Authors: Rezatofighi SH, Gould S, Vo BN, Mele K, Hughes WE, Hartley R.
Journal: Inf Process Med Imaging (2013): 110