The CytoWatch™ Endogenous Biotin Blocking Kit is designed to reduce background signal in immunohistochemistry (IHC), immunofluorescence (IF), and other avidin–biotin based detection systems by effectively blocking endogenous biotin. Endogenous biotin is often present in cells and tissues where it can lead to nonspecific binding and false-positive staining when using biotinylated probes.

This kit includes two components: a streptavidin solution to bind all endogenous biotin molecules and a subsequent biotin solution to block unoccupied streptavidin sites. This two-step blocking strategy ensures complete suppression of background caused by endogenous biotin, enabling higher specificity and clarity in staining results. The CytoWatch™ kit is easy to use and integrates seamlessly into existing IHC and IF protocols.