CYTOXite™ Green Dead Cell Stain is a membrane-impermeant fluorescent dye formulated to detect disruption of cell membrane integrity and quantify cell death in real time. The dye is excluded from healthy cells with intact membranes, resulting in little or no intrinsic fluorescence. When cells lose membrane integrity, the dye enters and binds to cellular DNA, producing a significant increase in fluorescence signal that enables sensitive detection of non-viable cells.

The stain is compatible with mix-and-read assay formats and supports continuous monitoring of cytotoxicity without the need for washing steps. It can be used alone or in combination with other assays for multiplexed analysis of cell health, including proliferation and apoptosis. The reagent is suitable for high-throughput applications and fluorescence-based detection platforms using standard 488 nm excitation.