D-Luciferin ethyl ester CAS 135251-85-3

IVIS images of 3 mice 20 d and 30 d after subcutaneous injection of 2 × 107 C3-luc cells. Chloral hydrate (10%) was intraperitoneally injected at 100 μL/mouse to anesthetize the mice. D-luciferin at a concentration of 150 mg/kg in sterile phosphate-buffered saline was injected after they were fully anesthetized. Animals were imaged for 180 s at a binning value of 8 and an FVO of 12.8 cm, 10 min after the injection of D-luciferin. Statistical analysis of photon levels was performed using an unpaired t-test (P < 0.05). Results of statistical analysis indicated that tumor size increased with time, and the correlation was significant. Source: <b>C3-Luc Cells Are an Excellent Model for Evaluation of Cellular Immunity following HPV16L1 Vaccination</b>. by Li L-L, Wang H-R, Zhou Z-Y, Luo J, Wang X-L, Xiao X-Q, et al. <em>PLoS ONE</em> 11(2). Feb. 2016.

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Physical properties

Molecular weight	308.38
Solvent	DMSO

Spectral properties

Excitation (nm)	335
Emission (nm)	530

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41105331

Overview SDS Protocol

See also: Enzymes, Luciferases, Oxidases

CAS

135251-85-3

Molecular weight

308.38

Excitation (nm)

335

Emission (nm)

530

D-Luciferin ethyl ester contains a methyl moiety attached at the carboxy position, thus preventing it be recognized by the firefly luciferase enzyme. However, the ester is hydrolyzed effectively by lipase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin methyl ester is a sensitive substrate for the chemiluminescent measurement of lipase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin ethyl ester *CAS 135251-85-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	324.275 µL	1.621 mL	3.243 mL	16.214 mL	32.428 mL
5 mM	64.855 µL	324.275 µL	648.55 µL	3.243 mL	6.486 mL
10 mM	32.428 µL	162.138 µL	324.275 µL	1.621 mL	3.243 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	335
Emission (nm)	530

Product Family

Name	Excitation (nm)	Emission (nm)
D-Luciferin methyl ester CAS 73918-26-0	335	530

Images

Figure 1. Chemical structure for D-Luciferin ethyl ester *CAS 135251-85-3*

Figure 2. IVIS images of 3 mice 20 d and 30 d after subcutaneous injection of 2 × 107 C3-luc cells. Chloral hydrate (10%) was intraperitoneally injected at 100 μL/mouse to anesthetize the mice. D-luciferin at a concentration of 150 mg/kg in sterile phosphate-buffered saline was injected after they were fully anesthetized. Animals were imaged for 180 s at a binning value of 8 and an FVO of 12.8 cm, 10 min after the injection of D-luciferin. Statistical analysis of photon levels was performed using an unpaired t-test (P < 0.05). Results of statistical analysis indicated that tumor size increased with time, and the correlation was significant. Source: C3-Luc Cells Are an Excellent Model for Evaluation of Cellular Immunity following HPV16L1 Vaccination. by Li L-L, Wang H-R, Zhou Z-Y, Luo J, Wang X-L, Xiao X-Q, et al. PLoS ONE 11(2). Feb. 2016.

Citations

View all 6 citations: Citation Explorer

Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9

Genome-wide microRNA analysis identifies miR-188-3p as novel prognostic marker and molecular factor involved in colorectal carcinogenesis
Authors: Pichler, Martin and Stiegelbauer, Verena and Vychytilova-Faltejskova, Petra and Ivan, Cristina and Ling, Hui and Winter, Elke and Zhang, Xinna and Goblirsch, Matthew and Wulf-Goldenberg, Annika and Ohtsuka, Masahisa and others, undefined
Journal: American Association for Cancer Research (2016): clincanres--0497

C3-Luc Cells Are an Excellent Model for Evaluation of Cellular Immunity following HPV16L1 Vaccination
Authors: Li, Li-Li and Wang, He-Rong and Zhou, Zhi-Yi and Luo, Jing and Wang, Xiao-Li and Xiao, Xiang-Qian and Zhou, Yu-Bai and Zeng, Yi
Journal: PloS one (2016): e0149748

Identification of a novel protein kinase a inhibitor by bioluminescence-based screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969--1974

Identification of a Novel Protein Kinase A Inhibitor by Bioluminescence-Based Screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969--1974

Discovery of novel adenylyl cyclase inhibitor by cell-based screening
Authors: Mano, Hiroki and Ishimoto, Tetsuya and Okada, Takuya and Toyooka, Naoki and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2014): 1689--1693

References

View all 113 references: Citation Explorer

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Journal: Biochem J (2006): 305

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Journal: Biol Pharm Bull (2006): 560

Expression of firefly luciferase in Candida albicans and its use in the selection of stable transformants
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Journal: Microb Pathog (2006): 69

PET imaging and optical imaging with D-luciferin [11C]methyl ester and D-luciferin [11C]methyl ether of luciferase gene expression in tumor xenografts of living mice
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Journal: Bioorg Med Chem Lett (2006): 331

Bioluminescence of monolayers of firefly luciferase immobilized on graphite
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Journal: Langmuir (2006): 5451

Bovine serum albumin displays luciferase-like activity in presence of luciferyl adenylate: insights on the origin of protoluciferase activity and bioluminescence colours
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Journal: Luminescence (2006): 262

Noninvasive bioluminescence imaging of luciferase expressing intracranial U87 xenografts: correlation with magnetic resonance imaging determined tumor volume and longitudinal use in assessing tumor growth and antiangiogenic treatment effect
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Journal: Neurosurgery (2006): 365

Two different domains of the luciferase gene in the heterotrophic dinoflagellate Noctiluca scintillans occur as two separate genes in photosynthetic species
Authors: Liu L, Hastings JW.
Journal: Proc Natl Acad Sci U S A. (2006)

Effect of different salts and detergents on luciferin-luciferase luminescence of the enchytraeid Fridericia heliota
Authors: Rodionova NS, Petushkov VN.
Journal: J Photochem Photobiol B (2006): 123

Evaluation of firefly luciferase bioluminescence mediated photodynamic toxicity in cancer cells
Authors: Schipper ML, Patel MR, Gambhir SS.
Journal: Mol Imaging Biol (2006): 218