D-Luciferin ethyl ester *CAS 135251-85-3*
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Additional ordering information
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| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 308.38 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 385 |
| Emission (nm) | 529 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 41105331 |
| Overview |  SDSProtocol |
See also: Luciferases, Oxidases
CAS 135251-85-3 | Molecular weight 308.38 | Excitation (nm) 385 | Emission (nm) 529 |
D-Luciferin ethyl ester contains a methyl moiety attached at the carboxy position, thus preventing it be recognized by the firefly luciferase enzyme. However, the ester is hydrolyzed effectively by lipase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin methyl ester is a sensitive substrate for the chemiluminescent measurement of lipase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin ethyl ester *CAS 135251-85-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 324.275 µL | 1.621 mL | 3.243 mL | 16.214 mL | 32.428 mL |
| 5 mM | 64.855 µL | 324.275 µL | 648.55 µL | 3.243 mL | 6.486 mL |
| 10 mM | 32.428 µL | 162.138 µL | 324.275 µL | 1.621 mL | 3.243 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Figure 1. Chemical structure for D-Luciferin ethyl ester *CAS 135251-85-3*
Figure 2. IVIS images of 3 mice 20 d and 30 d after subcutaneous injection of 2 × 107 C3-luc cells. Chloral hydrate (10%) was intraperitoneally injected at 100 μL/mouse to anesthetize the mice. D-luciferin at a concentration of 150 mg/kg in sterile phosphate-buffered saline was injected after they were fully anesthetized. Animals were imaged for 180 s at a binning value of 8 and an FVO of 12.8 cm, 10 min after the injection of D-luciferin. Statistical analysis of photon levels was performed using an unpaired t-test (P < 0.05). Results of statistical analysis indicated that tumor size increased with time, and the correlation was significant. Source: C3-Luc Cells Are an Excellent Model for Evaluation of Cellular Immunity following HPV16L1 Vaccination. by Li L-L, Wang H-R, Zhou Z-Y, Luo J, Wang X-L, Xiao X-Q, et al. PLoS ONE 11(2). Feb. 2016.
Citations
View all 8 citations: Citation Explorer
GRAS family member LATERAL SUPPRESSOR regulates the initiation and morphogenesis of watermelon lateral organs
Authors: Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,
Journal: Plant Physiology (2023): kiad445
Authors: Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,
Journal: Plant Physiology (2023): kiad445
Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625
Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9
Genome-wide microRNA analysis identifies miR-188-3p as novel prognostic marker and molecular factor involved in colorectal carcinogenesis
Authors: Pichler, Martin and Stiegelbauer, Verena and Vychytilova-Faltejskova, Petra and Ivan, Cristina and Ling, Hui and Winter, Elke and Zhang, Xinna and Goblirsch, Matthew and Wulf-Goldenberg, Annika and Ohtsuka, Masahisa and others, undefined
Journal: American Association for Cancer Research (2016): clincanres--0497
Authors: Pichler, Martin and Stiegelbauer, Verena and Vychytilova-Faltejskova, Petra and Ivan, Cristina and Ling, Hui and Winter, Elke and Zhang, Xinna and Goblirsch, Matthew and Wulf-Goldenberg, Annika and Ohtsuka, Masahisa and others, undefined
Journal: American Association for Cancer Research (2016): clincanres--0497
C3-Luc Cells Are an Excellent Model for Evaluation of Cellular Immunity following HPV16L1 Vaccination
Authors: Li, Li-Li and Wang, He-Rong and Zhou, Zhi-Yi and Luo, Jing and Wang, Xiao-Li and Xiao, Xiang-Qian and Zhou, Yu-Bai and Zeng, Yi
Journal: PloS one (2016): e0149748
Authors: Li, Li-Li and Wang, He-Rong and Zhou, Zhi-Yi and Luo, Jing and Wang, Xiao-Li and Xiao, Xiang-Qian and Zhou, Yu-Bai and Zeng, Yi
Journal: PloS one (2016): e0149748
Identification of a novel protein kinase a inhibitor by bioluminescence-based screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969--1974
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969--1974
Identification of a Novel Protein Kinase A Inhibitor by Bioluminescence-Based Screening
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969--1974
Authors: Ishimoto, Tetsuya and Azechi, Kenji and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2015): 1969--1974
Discovery of novel adenylyl cyclase inhibitor by cell-based screening
Authors: Mano, Hiroki and Ishimoto, Tetsuya and Okada, Takuya and Toyooka, Naoki and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2014): 1689--1693
Authors: Mano, Hiroki and Ishimoto, Tetsuya and Okada, Takuya and Toyooka, Naoki and Mori, Hisashi
Journal: Biological and Pharmaceutical Bulletin (2014): 1689--1693
References
View all 113 references: Citation Explorer
Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance
Authors: Law GH, G and elman OA, Tisi LC, Lowe CR, Murray JA.
Journal: Biochem J (2006): 305
Authors: Law GH, G and elman OA, Tisi LC, Lowe CR, Murray JA.
Journal: Biochem J (2006): 305
Determination of p-glycoprotein ATPase activity using luciferase
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Expression of firefly luciferase in Candida albicans and its use in the selection of stable transformants
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Authors: Doyle TC, Nawotka KA, Purchio AF, Akin AR, Francis KP, Contag PR.
Journal: Microb Pathog (2006): 69
PET imaging and optical imaging with D-luciferin [11C]methyl ester and D-luciferin [11C]methyl ether of luciferase gene expression in tumor xenografts of living mice
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Authors: Wang JQ, Pollok KE, Cai S, Stantz KM, Hutchins GD, Zheng QH.
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Bioluminescence of monolayers of firefly luciferase immobilized on graphite
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Journal: Langmuir (2006): 5451
Authors: Palomba S, Berovic N, Palmer RE.
Journal: Langmuir (2006): 5451
Bovine serum albumin displays luciferase-like activity in presence of luciferyl adenylate: insights on the origin of protoluciferase activity and bioluminescence colours
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Authors: Viviani VR, Ohmiya Y.
Journal: Luminescence (2006): 262
Noninvasive bioluminescence imaging of luciferase expressing intracranial U87 xenografts: correlation with magnetic resonance imaging determined tumor volume and longitudinal use in assessing tumor growth and antiangiogenic treatment effect
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Authors: Szentirmai O, Baker CH, Lin N, Szucs S, Takahashi M, Kiryu S, Kung AL, Mulligan RC, Carter BS.
Journal: Neurosurgery (2006): 365
Two different domains of the luciferase gene in the heterotrophic dinoflagellate Noctiluca scintillans occur as two separate genes in photosynthetic species
Authors: Liu L, Hastings JW.
Journal: Proc Natl Acad Sci U S A. (2006)
Authors: Liu L, Hastings JW.
Journal: Proc Natl Acad Sci U S A. (2006)
Effect of different salts and detergents on luciferin-luciferase luminescence of the enchytraeid Fridericia heliota
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Authors: Rodionova NS, Petushkov VN.
Journal: J Photochem Photobiol B (2006): 123
Evaluation of firefly luciferase bioluminescence mediated photodynamic toxicity in cancer cells
Authors: Schipper ML, Patel MR, Gambhir SS.
Journal: Mol Imaging Biol (2006): 218
Authors: Schipper ML, Patel MR, Gambhir SS.
Journal: Mol Imaging Biol (2006): 218
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