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D-Luciferin ethyl ester *CAS 135251-85-3*
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Application notes
D-Luciferin ethyl ester contains a methyl moiety attached at the carboxy position, thus preventing it be recognized by the firefly luciferase enzyme. However, the ester is hydrolyzed effectively by lipase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin methyl ester is a sensitive substrate for the chemiluminescent measurement of lipase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin ethyl ester *CAS 135251-85-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 324.275 µL | 1.621 mL | 3.243 mL | 16.214 mL | 32.428 mL |
| 5 mM | 64.855 µL | 324.275 µL | 648.55 µL | 3.243 mL | 6.486 mL |
| 10 mM | 32.428 µL | 162.138 µL | 324.275 µL | 1.621 mL | 3.243 mL |
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| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| D-Luciferin methyl ester *CAS 73918-26-0* | 385 | 529 |
Citations
View all 9 citations: Citation Explorer
Bmal1 integrates circadian function and temperature sensing in the suprachiasmatic nucleus
Authors: Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco
Journal: Proceedings of the National Academy of Sciences (2024): e2316646121
Authors: Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco
Journal: Proceedings of the National Academy of Sciences (2024): e2316646121
GRAS family member LATERAL SUPPRESSOR regulates the initiation and morphogenesis of watermelon lateral organs
Authors: Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,
Journal: Plant Physiology (2023): kiad445
Authors: Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,
Journal: Plant Physiology (2023): kiad445
Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625
Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9
Genome-wide microRNA analysis identifies miR-188-3p as novel prognostic marker and molecular factor involved in colorectal carcinogenesis
Authors: Pichler, Martin and Stiegelbauer, Verena and Vychytilova-Faltejskova, Petra and Ivan, Cristina and Ling, Hui and Winter, Elke and Zhang, Xinna and Goblirsch, Matthew and Wulf-Goldenberg, Annika and Ohtsuka, Masahisa and others, undefined
Journal: American Association for Cancer Research (2016): clincanres--0497
Authors: Pichler, Martin and Stiegelbauer, Verena and Vychytilova-Faltejskova, Petra and Ivan, Cristina and Ling, Hui and Winter, Elke and Zhang, Xinna and Goblirsch, Matthew and Wulf-Goldenberg, Annika and Ohtsuka, Masahisa and others, undefined
Journal: American Association for Cancer Research (2016): clincanres--0497
References
View all 113 references: Citation Explorer
Bovine serum albumin displays luciferase-like activity in presence of luciferyl adenylate: insights on the origin of protoluciferase activity and bioluminescence colours
Authors: Viviani VR, Ohmiya Y.
Journal: Luminescence (2006): 262
Authors: Viviani VR, Ohmiya Y.
Journal: Luminescence (2006): 262
cDNA cloning, expression and homology modeling of a luciferase from the firefly Lampyroidea maculata
Authors: Emamzadeh AR, Hosseinkhani S, Sadeghizadeh M, Nikkhah M, Chaichi MJ, Mortazavi M.
Journal: J Biochem Mol Biol (2006): 578
Authors: Emamzadeh AR, Hosseinkhani S, Sadeghizadeh M, Nikkhah M, Chaichi MJ, Mortazavi M.
Journal: J Biochem Mol Biol (2006): 578
Cloning and characterization of the homologous genes of firefly luciferase in the mealworm beetle, Tenebrio molitor
Authors: Oba Y, Sato M, Inouye S.
Journal: Insect Mol Biol (2006): 293
Authors: Oba Y, Sato M, Inouye S.
Journal: Insect Mol Biol (2006): 293
Structural basis for the spectral difference in luciferase bioluminescence
Authors: Nakatsu T, Ichiyama S, Hiratake J, Saldanha A, Kobashi N, Sakata K, Kato H.
Journal: Nature (2006): 372
Authors: Nakatsu T, Ichiyama S, Hiratake J, Saldanha A, Kobashi N, Sakata K, Kato H.
Journal: Nature (2006): 372
Firefly luciferase produces hydrogen peroxide as a coproduct in dehydroluciferyl adenylate formation
Authors: Fraga H, Fern and es D, Novotny J, Fontes R, Esteves da Silva JC.
Journal: Chembiochem (2006): 929
Authors: Fraga H, Fern and es D, Novotny J, Fontes R, Esteves da Silva JC.
Journal: Chembiochem (2006): 929