D-Luciferin | AAT Bioquest

D-Luciferin ethyl ester

CAS 135251-85-3

D-Luciferin ethyl ester contains a methyl moiety attached at the carboxy position, thus preventing it be recognized by the firefly luciferase enzyme. However, the ester is hydrolyzed effectively by lipase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin methyl ester is a sensitive substrate for the chemiluminescent measurement of lipase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
12515	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin ethyl ester *CAS 135251-85-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	324.275 µL	1.621 mL	3.243 mL	16.214 mL	32.428 mL
5 mM	64.855 µL	324.275 µL	648.55 µL	3.243 mL	6.486 mL
10 mM	32.428 µL	162.138 µL	324.275 µL	1.621 mL	3.243 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	308.38 g/mol	=	mL	x	mM	=	mmol

Citations

View all 10 citations: Citation Explorer

Extracellular vesicle surface display of $\alpha$PD-L1 and $\alpha$CD3 antibodies via engineered late domain-based scaffold to activate T-cell anti-tumor immunity

Authors:

Chen, Rui and Kang, Ziqin and Li, Wenhao and Xu, Tianshu and Wang, Yongqiang and Jiang, Qiming and Wang, Yuepeng and Huang, Zixian and Xu, Xiaoding and Huang, Zhiquan

Journal:

Journal of Extracellular Vesicles (2024): e12490

Bmal1 integrates circadian function and temperature sensing in the suprachiasmatic nucleus

Authors:

Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco

Journal:

Proceedings of the National Academy of Sciences (2024): e2316646121

GRAS family member LATERAL SUPPRESSOR regulates the initiation and morphogenesis of watermelon lateral organs

Authors:

Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,

Journal:

Plant Physiology (2023): kiad445

Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea

Authors:

Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,

Journal:

Bioorganic Chemistry (2023): 106625

Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition

Authors:

Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan

Journal:

Oncogenesis (2022): 1--9

References

View all 113 references: Citation Explorer

Bioluminescence of monolayers of firefly luciferase immobilized on graphite

Authors:

Palomba S, Berovic N, Palmer RE.

Journal:

Langmuir (2006): 5451

cDNA cloning, expression and homology modeling of a luciferase from the firefly Lampyroidea maculata

Authors:

Emamzadeh AR, Hosseinkhani S, Sadeghizadeh M, Nikkhah M, Chaichi MJ, Mortazavi M.

Journal:

J Biochem Mol Biol (2006): 578

Cloning and characterization of the homologous genes of firefly luciferase in the mealworm beetle, Tenebrio molitor

Authors:

Oba Y, Sato M, Inouye S.

Journal:

Insect Mol Biol (2006): 293

Structural basis for the spectral difference in luciferase bioluminescence

Authors:

Nakatsu T, Ichiyama S, Hiratake J, Saldanha A, Kobashi N, Sakata K, Kato H.

Journal:

Nature (2006): 372

Firefly luciferase produces hydrogen peroxide as a coproduct in dehydroluciferyl adenylate formation

Authors:

Fraga H, Fern and es D, Novotny J, Fontes R, Esteves da Silva JC.

Journal:

Chembiochem (2006): 929

Physical properties

Molecular weight	308.38
Solvent	DMSO

Spectral properties

Excitation (nm)	385
Emission (nm)	529

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41105331
CAS	135251-85-3

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Page updated on December 13, 2024