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D-VLK-AMC is a sensitive, highly specific fluorescent substrate for plasmin. It can be used with a fluorescence microscope, fluorescence microplate or flow cytometer.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of D-VLK-AMC [D-Val-Leu-Lys-AMC] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 134.465 µL | 672.323 µL | 1.345 mL | 6.723 mL | 13.446 mL |
| 5 mM | 26.893 µL | 134.465 µL | 268.929 µL | 1.345 mL | 2.689 mL |
| 10 mM | 13.446 µL | 67.232 µL | 134.465 µL | 672.323 µL | 1.345 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| D-Ala-AMC *CAS 201847-52-1* | 341 | 441 |
Citations
View all 6 citations: Citation Explorer
Study on Optimal Production Conditions of Fibrinolytic Kinase Derived from the Nereid Worm, Perinereis aibuhitensis Grub
Authors: Song, Tuo and Cheng, Jun and Diao, Xiaozhen and Man, Yang and Chen, Boyu and Zhang, Haixing and Elango, Jeevithan and Wu, Wenhui
Journal: Fermentation (2024): 468
Authors: Song, Tuo and Cheng, Jun and Diao, Xiaozhen and Man, Yang and Chen, Boyu and Zhang, Haixing and Elango, Jeevithan and Wu, Wenhui
Journal: Fermentation (2024): 468
Computer-Aided Engineering of Staphylokinase Toward Enhanced Affinity and Selectivity for Plasmin
Authors: Nikitin, Dmitri and Mican, Jan and Toul, Martin and Bednar, David and Peskova, Michaela and Kittova, Patricia and Thalerova, Sandra and Vitecek, Jan and Damborsky, Jiri and Mikulik, Robert and others,
Journal: Computational and Structural Biotechnology Journal (2022)
Authors: Nikitin, Dmitri and Mican, Jan and Toul, Martin and Bednar, David and Peskova, Michaela and Kittova, Patricia and Thalerova, Sandra and Vitecek, Jan and Damborsky, Jiri and Mikulik, Robert and others,
Journal: Computational and Structural Biotechnology Journal (2022)
Diabetogenic agent alloxan is a proteasome inhibitor
Authors: Zhou, Wenjuan and Wei, Lingling and Xiao, Ting and Lai, Chunyou and Peng, Min and Xu, Lingli and Luo, Xiangwei and Deng, Shaoping and Zhang, Fengxue
Journal: Biochemical and Biophysical Research Communications (2017): 400--406
Authors: Zhou, Wenjuan and Wei, Lingling and Xiao, Ting and Lai, Chunyou and Peng, Min and Xu, Lingli and Luo, Xiangwei and Deng, Shaoping and Zhang, Fengxue
Journal: Biochemical and Biophysical Research Communications (2017): 400--406
Delineation of molecular pathways involved in cardiomyopathies caused by troponin T mutations
Authors: Gilda, Jennifer E and Lai, Xianyin and Witzmann, Frank A and Gomes, Aldrin V
Journal: Molecular & Cellular Proteomics (2016): 1962--1981
Authors: Gilda, Jennifer E and Lai, Xianyin and Witzmann, Frank A and Gomes, Aldrin V
Journal: Molecular & Cellular Proteomics (2016): 1962--1981
Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts
Authors: Ghosh, Rajeshwary and Goswami, Sumanta K and Feitoza, Luis Felipe BB and Hammock, Bruce and Gomes, Aldrin V
Journal: International Journal of Cardiology (2016): 923--935
Authors: Ghosh, Rajeshwary and Goswami, Sumanta K and Feitoza, Luis Felipe BB and Hammock, Bruce and Gomes, Aldrin V
Journal: International Journal of Cardiology (2016): 923--935
References
View all 36 references: Citation Explorer
Enzymatic properties of the 20S proteasome in wheat endosperm and its biochemical characteristics after seed imbibition
Authors: Shi C, Rui Q, Xu LL.
Journal: Plant Biol (Stuttg) (2009): 849
Authors: Shi C, Rui Q, Xu LL.
Journal: Plant Biol (Stuttg) (2009): 849
Comparative expression analysis and characterization of 20S proteasomes in human intestinal tissues: The proteasome pattern as diagnostic tool for IBD patients
Authors: Visekruna A, Joeris T, Schmidt N, Lawrenz M, Ritz JP, Buhr HJ, Steinhoff U.
Journal: Inflamm Bowel Dis (2009): 526
Authors: Visekruna A, Joeris T, Schmidt N, Lawrenz M, Ritz JP, Buhr HJ, Steinhoff U.
Journal: Inflamm Bowel Dis (2009): 526
Quinone reductase acts as a redox switch of the 20S yeast proteasome
Authors: Sollner S, Schober M, Wagner A, Prem A, Lorkova L, Palfey BA, Groll M, Macheroux P.
Journal: EMBO Rep (2009): 65
Authors: Sollner S, Schober M, Wagner A, Prem A, Lorkova L, Palfey BA, Groll M, Macheroux P.
Journal: EMBO Rep (2009): 65
Alveolar extracellular 20S proteasome in patients with acute respiratory distress syndrome
Authors: Sixt SU, Adamzik M, Spyrka D, Saul B, Hakenbeck J, Wohlschlaeger J, Costabel U, Kloss A, Giesebrecht J, Dahlmann B, Peters J.
Journal: Am J Respir Crit Care Med (2009): 1098
Authors: Sixt SU, Adamzik M, Spyrka D, Saul B, Hakenbeck J, Wohlschlaeger J, Costabel U, Kloss A, Giesebrecht J, Dahlmann B, Peters J.
Journal: Am J Respir Crit Care Med (2009): 1098
BCL-2 family regulation by the 20S proteasome inhibitor bortezomib
Authors: Fennell DA, Chacko A, Mutti L.
Journal: Oncogene (2008): 1189
Authors: Fennell DA, Chacko A, Mutti L.
Journal: Oncogene (2008): 1189
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