D-VLK-AMC [D-Val-Leu-Lys-AMC]

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Physical properties

Molecular weight	743.69
Solvent	DMSO

Spectral properties

Excitation (nm)	341
Emission (nm)	441

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

743.69

Excitation (nm)

341

Emission (nm)

441

D-VLK-AMC is a sensitive, highly specific fluorescent substrate for plasmin. It can be used with a fluorescence microscope, fluorescence microplate or flow cytometer.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of D-VLK-AMC [D-Val-Leu-Lys-AMC] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	134.465 µL	672.323 µL	1.345 mL	6.723 mL	13.446 mL
5 mM	26.893 µL	134.465 µL	268.929 µL	1.345 mL	2.689 mL
10 mM	13.446 µL	67.232 µL	134.465 µL	672.323 µL	1.345 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	341
Emission (nm)	441

Product Family

Name	Excitation (nm)	Emission (nm)
D-Ala-AMC CAS 201847-52-1	341	441

Images

Figure 1. Chemical structure for D-VLK-AMC [D-Val-Leu-Lys-AMC]

Citations

View all 5 citations: Citation Explorer

Computer-Aided Engineering of Staphylokinase Toward Enhanced Affinity and Selectivity for Plasmin
Authors: Nikitin, Dmitri and Mican, Jan and Toul, Martin and Bednar, David and Peskova, Michaela and Kittova, Patricia and Thalerova, Sandra and Vitecek, Jan and Damborsky, Jiri and Mikulik, Robert and others,
Journal: Computational and Structural Biotechnology Journal (2022)

Diabetogenic agent alloxan is a proteasome inhibitor
Authors: Zhou, Wenjuan and Wei, Lingling and Xiao, Ting and Lai, Chunyou and Peng, Min and Xu, Lingli and Luo, Xiangwei and Deng, Shaoping and Zhang, Fengxue
Journal: Biochemical and Biophysical Research Communications (2017): 400--406

Delineation of molecular pathways involved in cardiomyopathies caused by troponin T mutations
Authors: Gilda, Jennifer E and Lai, Xianyin and Witzmann, Frank A and Gomes, Aldrin V
Journal: Molecular & Cellular Proteomics (2016): 1962--1981

Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts
Authors: Ghosh, Rajeshwary and Goswami, Sumanta K and Feitoza, Luis Felipe BB and Hammock, Bruce and Gomes, Aldrin V
Journal: International Journal of Cardiology (2016): 923--935

Advanced-glycation-end-product-induced formation of immunoproteasomes: involvement of RAGE and Jak2/STAT1
Authors: Grimm, Stefanie and Ott, Christiane and Hörlacher, Melanie and Weber, Daniela and Höhn, Annika and Grune, Tilman
Journal: Biochemical Journal (2012): 127--139

References

View all 36 references: Citation Explorer

Alveolar extracellular 20S proteasome in patients with acute respiratory distress syndrome
Authors: Sixt SU, Adamzik M, Spyrka D, Saul B, Hakenbeck J, Wohlschlaeger J, Costabel U, Kloss A, Giesebrecht J, Dahlmann B, Peters J.
Journal: Am J Respir Crit Care Med (2009): 1098

Quinone reductase acts as a redox switch of the 20S yeast proteasome
Authors: Sollner S, Schober M, Wagner A, Prem A, Lorkova L, Palfey BA, Groll M, Macheroux P.
Journal: EMBO Rep (2009): 65

Comparative expression analysis and characterization of 20S proteasomes in human intestinal tissues: The proteasome pattern as diagnostic tool for IBD patients
Authors: Visekruna A, Joeris T, Schmidt N, Lawrenz M, Ritz JP, Buhr HJ, Steinhoff U.
Journal: Inflamm Bowel Dis (2009): 526

Enzymatic properties of the 20S proteasome in wheat endosperm and its biochemical characteristics after seed imbibition
Authors: Shi C, Rui Q, Xu LL.
Journal: Plant Biol (Stuttg) (2009): 849

BCL-2 family regulation by the 20S proteasome inhibitor bortezomib
Authors: Fennell DA, Chacko A, Mutti L.
Journal: Oncogene (2008): 1189

Toward a full characterization of the human 20S proteasome subunits and their isoforms by a combination of proteomic approaches
Authors: Uttenweiler-Joseph S, Claverol S, Sylvius L, Bousquet-Dubouch MP, Burlet-Schiltz O, Monsarrat B.
Journal: Methods Mol Biol (2008): 111

Circulating 20S proteasome levels in patients with mixed connective tissue disease and systemic lupus erythematosus
Authors: Majetschak M, Perez M, Sorell LT, Lam J, Maldonado ME, Hoffman RW.
Journal: Clin Vaccine Immunol (2008): 1489

The 20S proteasome of Schistosoma mansoni: a proteomic analysis
Authors: Castro-Borges W, Cartwright J, Ashton PD, Braschi S, Guerra Sa R, Rodrigues V, Wilson RA, Curwen RS.
Journal: Proteomics (2007): 1065

Design and synthesis of a novel class of furan-based molecules as potential 20S proteasome inhibitors
Authors: Fu Y, Xu B, Zou X, Ma C, Yang X, Mou K, Fu G, Lu Y, Xu P.
Journal: Bioorg Med Chem Lett (2007): 1102

beta-Subunit appendages promote 20S proteasome assembly by overcoming an Ump1-dependent checkpoint
Authors: Li X, Kusmierczyk AR, Wong P, Emili A, Hochstrasser M.
Journal: Embo J (2007): 2339