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D-VLK-AMC [D-Val-Leu-Lys-AMC]

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Physical properties
Molecular weight743.69
Spectral properties
Excitation (nm)341
Emission (nm)441
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Coumarins, Proteases
Molecular weight
Excitation (nm)
Emission (nm)
D-VLK-AMC is a sensitive, highly specific fluorescent substrate for plasmin. It can be used with a fluorescence microscope, fluorescence microplate or flow cytometer.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of D-VLK-AMC [D-Val-Leu-Lys-AMC] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM134.465 µL672.323 µL1.345 mL6.723 mL13.446 mL
5 mM26.893 µL134.465 µL268.929 µL1.345 mL2.689 mL
10 mM13.446 µL67.232 µL134.465 µL672.323 µL1.345 mL

Molarity calculator

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Spectral properties

Excitation (nm)341
Emission (nm)441

Product Family

NameExcitation (nm)Emission (nm)
D-Ala-AMC *CAS 201847-52-1*341441



View all 5 citations: Citation Explorer
Computer-Aided Engineering of Staphylokinase Toward Enhanced Affinity and Selectivity for Plasmin
Authors: Nikitin, Dmitri and Mican, Jan and Toul, Martin and Bednar, David and Peskova, Michaela and Kittova, Patricia and Thalerova, Sandra and Vitecek, Jan and Damborsky, Jiri and Mikulik, Robert and others,
Journal: Computational and Structural Biotechnology Journal (2022)
Diabetogenic agent alloxan is a proteasome inhibitor
Authors: Zhou, Wenjuan and Wei, Lingling and Xiao, Ting and Lai, Chunyou and Peng, Min and Xu, Lingli and Luo, Xiangwei and Deng, Shaoping and Zhang, Fengxue
Journal: Biochemical and Biophysical Research Communications (2017): 400--406
Delineation of molecular pathways involved in cardiomyopathies caused by troponin T mutations
Authors: Gilda, Jennifer E and Lai, Xianyin and Witzmann, Frank A and Gomes, Aldrin V
Journal: Molecular &amp; Cellular Proteomics (2016): 1962--1981
Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts
Authors: Ghosh, Rajeshwary and Goswami, Sumanta K and Feitoza, Luis Felipe BB and Hammock, Bruce and Gomes, Aldrin V
Journal: International Journal of Cardiology (2016): 923--935
Advanced-glycation-end-product-induced formation of immunoproteasomes: involvement of RAGE and Jak2/STAT1
Authors: Grimm, Stefanie and Ott, Christiane and H&ouml;rlacher, Melanie and Weber, Daniela and H&ouml;hn, Annika and Grune, Tilman
Journal: Biochemical Journal (2012): 127--139


View all 36 references: Citation Explorer
Alveolar extracellular 20S proteasome in patients with acute respiratory distress syndrome
Authors: Sixt SU, Adamzik M, Spyrka D, Saul B, Hakenbeck J, Wohlschlaeger J, Costabel U, Kloss A, Giesebrecht J, Dahlmann B, Peters J.
Journal: Am J Respir Crit Care Med (2009): 1098
Quinone reductase acts as a redox switch of the 20S yeast proteasome
Authors: Sollner S, Schober M, Wagner A, Prem A, Lorkova L, Palfey BA, Groll M, Macheroux P.
Journal: EMBO Rep (2009): 65
Comparative expression analysis and characterization of 20S proteasomes in human intestinal tissues: The proteasome pattern as diagnostic tool for IBD patients
Authors: Visekruna A, Joeris T, Schmidt N, Lawrenz M, Ritz JP, Buhr HJ, Steinhoff U.
Journal: Inflamm Bowel Dis (2009): 526
Enzymatic properties of the 20S proteasome in wheat endosperm and its biochemical characteristics after seed imbibition
Authors: Shi C, Rui Q, Xu LL.
Journal: Plant Biol (Stuttg) (2009): 849
BCL-2 family regulation by the 20S proteasome inhibitor bortezomib
Authors: Fennell DA, Chacko A, Mutti L.
Journal: Oncogene (2008): 1189
Circulating 20S proteasome levels in patients with mixed connective tissue disease and systemic lupus erythematosus
Authors: Majetschak M, Perez M, Sorell LT, Lam J, Maldonado ME, Hoffman RW.
Journal: Clin Vaccine Immunol (2008): 1489
Toward a full characterization of the human 20S proteasome subunits and their isoforms by a combination of proteomic approaches
Authors: Uttenweiler-Joseph S, Claverol S, Sylvius L, Bousquet-Dubouch MP, Burlet-Schiltz O, Monsarrat B.
Journal: Methods Mol Biol (2008): 111
The 20S proteasome of Schistosoma mansoni: a proteomic analysis
Authors: Castro-Borges W, Cartwright J, Ashton PD, Braschi S, Guerra Sa R, Rodrigues V, Wilson RA, Curwen RS.
Journal: Proteomics (2007): 1065
Design and synthesis of a novel class of furan-based molecules as potential 20S proteasome inhibitors
Authors: Fu Y, Xu B, Zou X, Ma C, Yang X, Mou K, Fu G, Lu Y, Xu P.
Journal: Bioorg Med Chem Lett (2007): 1102
beta-Subunit appendages promote 20S proteasome assembly by overcoming an Ump1-dependent checkpoint
Authors: Li X, Kusmierczyk AR, Wong P, Emili A, Hochstrasser M.
Journal: Embo J (2007): 2339