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DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*

Chemical structure for DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*
Chemical structure for DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*
Ordering information
Price ()
Catalog Number2001
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight269.3
SolventDMSO
Spectral properties
Absorbance (nm)454
Correction Factor (280 nm)0.516
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
6268-49-1
Molecular weight
269.3
Absorbance (nm)
454
Correction Factor (280 nm)
0.516
DABCYL acid is one of the most popular acceptors for developing FRET-based nucleic acid probes suh as Molecular Beacon-based PCR probes. It is also used to prepare a variety of FRET peptides that are often used for the detection of various proteases. It is predominantly paired with EDANS (as fluorescence donor) in FRET-based fluorescent probes.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM371.333 µL1.857 mL3.713 mL18.567 mL37.133 mL
5 mM74.267 µL371.333 µL742.666 µL3.713 mL7.427 mL
10 mM37.133 µL185.667 µL371.333 µL1.857 mL3.713 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Absorbance (nm)454
Correction Factor (280 nm)0.516

Citations


View all 3 citations: Citation Explorer
Peptide Based Inhibitors of Protein binding to the Mitogen-Activated Protein Kinase Docking Groove
Authors: Alexa, Anita and Ember, Orsolya and Szab{\'o}, Ildik{\'o} and Mo'ath, Yousef and P{\'o}ti, {\'A}d{\'a}m L and Rem{\'e}nyi, Attila and B{\'a}n{\'o}czi, Zolt{\'a}n
Journal: Frontiers in molecular biosciences (2021): 629
Influence of the Dabcyl group on the cellular uptake of cationic peptides: short oligoarginines as efficient cell-penetrating peptides
Authors: Szab{\'o}, Ildik{\'o} and Illien, Fran{\c{c}}oise and D{\'o}kus, Levente E and Baranyai, Zsuzsa and B{\H{o}}sze, Szilvia and Ise, Shoko and Kawano, Kenichi and Sagan, Sandrine and Futaki, Shiroh and Hudecz, Ferenc and others,
Journal: Amino Acids (2021): 1--17
Protein N-terminal processing: substrate specificity of Escherichia coli and human methionine aminopeptidases
Authors: Xiao, Qing and Zhang, Feiran and Nacev, Benjamin A and Liu, Jun O and Pei, Dehua
Journal: Biochemistry (2010): 5588--5599

References


View all 34 references: Citation Explorer
Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Characterization and inhibition of SARS-coronavirus main protease
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
Fluorogenic peptide substrates containing benzoxazol-5-yl-alanine derivatives for kinetic assay of cysteine proteases
Authors: Szabelski M, Rogiewicz M, Wiczk W.
Journal: Anal Biochem (2005): 20
ADAM33 enzyme properties and substrate specificity
Authors: Zou J, Zhang R, Zhu F, Liu J, Madison V, Uml and SP., undefined
Journal: Biochemistry (2005): 4247
Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique
Authors: Chen S, Chen LL, Luo HB, Sun T, Chen J, Ye F, Cai JH, Shen JK, Shen X, Jiang HL.
Journal: Acta Pharmacol Sin (2005): 99
Fluorogenic peptide substrates for carboxydipeptidase activity of cathepsin B
Authors: Stachowiak K, Tokmina M, Karpinska A, Sosnowska R, Wiczk W.
Journal: Acta Biochim Pol (2004): 81
Synthesis of poly(ethylene glycol)-based saquinavir prodrug conjugates and assessment of release and anti-HIV-1 bioactivity using a novel protease inhibition assay
Authors: Gunaseelan S, Debrah O, Wan L, Leibowitz MJ, Rabson AB, Stein S, Sinko PJ.
Journal: Bioconjug Chem (2004): 1322
Highly sensitive intramolecularly quenched fluorogenic substrates for renin based on the combination of L-2-amino-3-(7-methoxy-4-coumaryl)propionic acid with 2,4-dinitrophenyl groups at various positions
Authors: Paschalidou K, Neumann U, Gerhartz B, Tzougraki C.
Journal: Biochem J (2004): 1031