DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*

DABCYL acid

4-((4-(Dimethylamino)phenyl)azo)benzoic acid; CAS 6268-49-1

DABCYL acid is one of the most popular acceptors for developing FRET-based nucleic acid probes suh as Molecular Beacon-based PCR probes. It is also used to prepare a variety of FRET peptides that are often used for the detection of various proteases. It is predominantly paired with EDANS (as fluorescence donor) in FRET-based fluorescent probes.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2001	5 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	371.333 µL	1.857 mL	3.713 mL	18.567 mL	37.133 mL
5 mM	74.267 µL	371.333 µL	742.666 µL	3.713 mL	7.427 mL
10 mM	37.133 µL	185.667 µL	371.333 µL	1.857 mL	3.713 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	269.3 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

N-hydroxysuccinimidyl derivatization reagents, friends or foes? From synthesis to derivatization of amino compounds and their analysis using liquid chromatography tandem mass spectrometry

Authors:

Zapata, Ernesto and B{\`u}i, Nguyễn Kim Ng{\^a}n and Selberg, Sigrid and Leito, Ivo and Herodes, Koit

Journal:

Discover Applied Sciences (2025): 1--14

Peptide Based Inhibitors of Protein binding to the Mitogen-Activated Protein Kinase Docking Groove

Authors:

Alexa, Anita and Ember, Orsolya and Szab{\'o}, Ildik{\'o} and Mo'ath, Yousef and P{\'o}ti, {\'A}d{\'a}m L and Rem{\'e}nyi, Attila and B{\'a}n{\'o}czi, Zolt{\'a}n

Journal:

Frontiers in molecular biosciences (2021): 629

Influence of the Dabcyl group on the cellular uptake of cationic peptides: short oligoarginines as efficient cell-penetrating peptides

Authors:

Szab{\'o}, Ildik{\'o} and Illien, Fran{\c{c}}oise and D{\'o}kus, Levente E and Baranyai, Zsuzsa and B{\H{o}}sze, Szilvia and Ise, Shoko and Kawano, Kenichi and Sagan, Sandrine and Futaki, Shiroh and Hudecz, Ferenc and others,

Journal:

Amino Acids (2021): 1--17

Protein N-terminal processing: substrate specificity of Escherichia coli and human methionine aminopeptidases

Authors:

Xiao, Qing and Zhang, Feiran and Nacev, Benjamin A and Liu, Jun O and Pei, Dehua

Journal:

Biochemistry (2010): 5588--5599

References

View all 34 references: Citation Explorer

Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum

Authors:

Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.

Journal:

Protein Expr Purif (2006): 88

Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus

Authors:

Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.

Journal:

Org Biomol Chem (2006): 3626

A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases

Authors:

Weimer S, Oertel K, Fuchsbauer HL.

Journal:

Anal Biochem (2006): 110

Characterization and inhibition of SARS-coronavirus main protease

Authors:

Liang PH., undefined

Journal:

Curr Top Med Chem (2006): 361

Fluorogenic peptide substrates containing benzoxazol-5-yl-alanine derivatives for kinetic assay of cysteine proteases

Authors:

Szabelski M, Rogiewicz M, Wiczk W.

Journal:

Anal Biochem (2005): 20

Physical properties

Molecular weight	269.3
Solvent	DMSO

Spectral properties

Absorbance (nm)	454
Correction factor (280 nm)	0.516

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	6268-49-1

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Page updated on October 12, 2025