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DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*

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Physical properties
Molecular weight269.3
Spectral properties
Absorbance (nm)454
Correction Factor (280 nm)0.516
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Absorbance (nm)
Correction Factor (280 nm)
DABCYL acid is one of the most popular acceptors for developing FRET-based nucleic acid probes suh as Molecular Beacon-based PCR probes. It is also used to prepare a variety of FRET peptides that are often used for the detection of various proteases. It is predominantly paired with EDANS (as fluorescence donor) in FRET-based fluorescent probes.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM371.333 µL1.857 mL3.713 mL18.567 mL37.133 mL
5 mM74.267 µL371.333 µL742.666 µL3.713 mL7.427 mL
10 mM37.133 µL185.667 µL371.333 µL1.857 mL3.713 mL

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Spectral properties

Absorbance (nm)454
Correction Factor (280 nm)0.516

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)Correction Factor (260 nm)
ICG acid789813230000-0.076-
Cy3NS acid *CAS 1032678-01-5*55556915000010.1510.0730.07
Cy5NS acid65167025000010.271, 0.420.030.02
Cy7NS acid7567792500000.30.0360.05
AMCA acid *CAS#: 106562-32-7*34643419000-0.153-
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7*3364555900-0.107-
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]3364555900-0.107-
BCECF acid *CAS#: 85138-49-4*50452790000---
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*534553810000.6110.090.24
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*499520710000.9210.110.30
Cy3B acid56057112000010.5810.0690.048
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*34344119000-0.190.25
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*5615721120000.7910.120.21
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*579603913000.6910.460.45
XFD514 acid51854380000-0.180.31
QXY7 acid [equivalent to QSY-7 acid]--900001-0.22-
XFD555 acid5535681550000.110.080.08
XFD647 acid6506712700000.3310.03-
XFD750 acid7527762900000.1210.04-
XFD700 acid6967192050000.2510.07-
OG514 acid [equivalent to Oregon Green® 514 carboxylic acid]51353385000-0.19-
Show More (12)



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Peptide Based Inhibitors of Protein binding to the Mitogen-Activated Protein Kinase Docking Groove
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Journal: Frontiers in molecular biosciences (2021): 629
Influence of the Dabcyl group on the cellular uptake of cationic peptides: short oligoarginines as efficient cell-penetrating peptides
Authors: Szab{\'o}, Ildik{\'o} and Illien, Fran{\c{c}}oise and D{\'o}kus, Levente E and Baranyai, Zsuzsa and B{\H{o}}sze, Szilvia and Ise, Shoko and Kawano, Kenichi and Sagan, Sandrine and Futaki, Shiroh and Hudecz, Ferenc and others,
Journal: Amino Acids (2021): 1--17
Protein N-terminal processing: substrate specificity of Escherichia coli and human methionine aminopeptidases
Authors: Xiao, Qing and Zhang, Feiran and Nacev, Benjamin A and Liu, Jun O and Pei, Dehua
Journal: Biochemistry (2010): 5588--5599


View all 34 references: Citation Explorer
Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Characterization and inhibition of SARS-coronavirus main protease
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
Fluorogenic peptide substrates containing benzoxazol-5-yl-alanine derivatives for kinetic assay of cysteine proteases
Authors: Szabelski M, Rogiewicz M, Wiczk W.
Journal: Anal Biochem (2005): 20
ADAM33 enzyme properties and substrate specificity
Authors: Zou J, Zhang R, Zhu F, Liu J, Madison V, Uml and SP., undefined
Journal: Biochemistry (2005): 4247
Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique
Authors: Chen S, Chen LL, Luo HB, Sun T, Chen J, Ye F, Cai JH, Shen JK, Shen X, Jiang HL.
Journal: Acta Pharmacol Sin (2005): 99
Fluorogenic peptide substrates for carboxydipeptidase activity of cathepsin B
Authors: Stachowiak K, Tokmina M, Karpinska A, Sosnowska R, Wiczk W.
Journal: Acta Biochim Pol (2004): 81
Synthesis of poly(ethylene glycol)-based saquinavir prodrug conjugates and assessment of release and anti-HIV-1 bioactivity using a novel protease inhibition assay
Authors: Gunaseelan S, Debrah O, Wan L, Leibowitz MJ, Rabson AB, Stein S, Sinko PJ.
Journal: Bioconjug Chem (2004): 1322
Highly sensitive intramolecularly quenched fluorogenic substrates for renin based on the combination of L-2-amino-3-(7-methoxy-4-coumaryl)propionic acid with 2,4-dinitrophenyl groups at various positions
Authors: Paschalidou K, Neumann U, Gerhartz B, Tzougraki C.
Journal: Biochem J (2004): 1031