DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 269.3 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 454 |
Correction Factor (280 nm) | 0.516 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Bioconjugation, Dye to Peptide Conjugation, Cell Structures and Organelles, Nucleus, Chemical Reagents, Förster Resonance Energy Transfer (FRET), Peptide and Oligonucleotide Labeling, Digital PCR, DNA and RNA Quantitation, Physiological Probes, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), Tide Quencher Dyes, PCR Detection of Viral DNA/RNA
CAS 6268-49-1 | Molecular weight 269.3 | Absorbance (nm) 454 | Correction Factor (280 nm) 0.516 |
DABCYL acid is one of the most popular acceptors for developing FRET-based nucleic acid probes suh as Molecular Beacon-based PCR probes. It is also used to prepare a variety of FRET peptides that are often used for the detection of various proteases. It is predominantly paired with EDANS (as fluorescence donor) in FRET-based fluorescent probes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 371.333 µL | 1.857 mL | 3.713 mL | 18.567 mL | 37.133 mL |
5 mM | 74.267 µL | 371.333 µL | 742.666 µL | 3.713 mL | 7.427 mL |
10 mM | 37.133 µL | 185.667 µL | 371.333 µL | 1.857 mL | 3.713 mL |
Molarity calculator
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Spectrum
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Spectral properties
Absorbance (nm) | 454 |
Correction Factor (280 nm) | 0.516 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) | Correction Factor (260 nm) |
ICG acid | 789 | 814 | - | - | 0.076 | - |
Cy3NS acid *CAS 1032678-01-5* | 555 | 569 | 1500001 | 0.151 | 0.073 | 0.07 |
Cy5NS acid | 651 | 670 | 2500001 | 0.271, 0.42 | 0.03 | 0.02 |
Cy7NS acid | 756 | 779 | 250000 | 0.3 | 0.036 | 0.05 |
AMCA acid *CAS#: 106562-32-7* | 346 | 434 | 19000 | - | 0.153 | - |
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7* | 336 | 455 | 5900 | - | 0.107 | - |
EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] | 336 | 455 | 5900 | - | 0.107 | - |
BCECF acid *CAS#: 85138-49-4* | 504 | 527 | 90000 | - | - | - |
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid* | 317 | 553 | 81000 | 0.611 | 0.09 | 0.24 |
Show More (11) |
Images
Citations
View all 3 citations: Citation Explorer
Peptide Based Inhibitors of Protein binding to the Mitogen-Activated Protein Kinase Docking Groove
Authors: Alexa, Anita and Ember, Orsolya and Szab{\'o}, Ildik{\'o} and Mo'ath, Yousef and P{\'o}ti, {\'A}d{\'a}m L and Rem{\'e}nyi, Attila and B{\'a}n{\'o}czi, Zolt{\'a}n
Journal: Frontiers in molecular biosciences (2021): 629
Authors: Alexa, Anita and Ember, Orsolya and Szab{\'o}, Ildik{\'o} and Mo'ath, Yousef and P{\'o}ti, {\'A}d{\'a}m L and Rem{\'e}nyi, Attila and B{\'a}n{\'o}czi, Zolt{\'a}n
Journal: Frontiers in molecular biosciences (2021): 629
Influence of the Dabcyl group on the cellular uptake of cationic peptides: short oligoarginines as efficient cell-penetrating peptides
Authors: Szab{\'o}, Ildik{\'o} and Illien, Fran{\c{c}}oise and D{\'o}kus, Levente E and Baranyai, Zsuzsa and B{\H{o}}sze, Szilvia and Ise, Shoko and Kawano, Kenichi and Sagan, Sandrine and Futaki, Shiroh and Hudecz, Ferenc and others,
Journal: Amino Acids (2021): 1--17
Authors: Szab{\'o}, Ildik{\'o} and Illien, Fran{\c{c}}oise and D{\'o}kus, Levente E and Baranyai, Zsuzsa and B{\H{o}}sze, Szilvia and Ise, Shoko and Kawano, Kenichi and Sagan, Sandrine and Futaki, Shiroh and Hudecz, Ferenc and others,
Journal: Amino Acids (2021): 1--17
Protein N-terminal processing: substrate specificity of Escherichia coli and human methionine aminopeptidases
Authors: Xiao, Qing and Zhang, Feiran and Nacev, Benjamin A and Liu, Jun O and Pei, Dehua
Journal: Biochemistry (2010): 5588--5599
Authors: Xiao, Qing and Zhang, Feiran and Nacev, Benjamin A and Liu, Jun O and Pei, Dehua
Journal: Biochemistry (2010): 5588--5599
References
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Journal: Anal Biochem (2006): 110
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Synthesis of poly(ethylene glycol)-based saquinavir prodrug conjugates and assessment of release and anti-HIV-1 bioactivity using a novel protease inhibition assay
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Highly sensitive intramolecularly quenched fluorogenic substrates for renin based on the combination of L-2-amino-3-(7-methoxy-4-coumaryl)propionic acid with 2,4-dinitrophenyl groups at various positions
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