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EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]

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Physical properties
Molecular weight266.32
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.107
Extinction coefficient (cm -1 M -1)5900
Excitation (nm)336
Emission (nm)455
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
50402-56-7
Molecular weight
266.32
Correction Factor (280 nm)
0.107
Extinction coefficient (cm -1 M -1)
5900
Excitation (nm)
336
Emission (nm)
455
EDANS is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM375.488 µL1.877 mL3.755 mL18.774 mL37.549 mL
5 mM75.098 µL375.488 µL750.976 µL3.755 mL7.51 mL
10 mM37.549 µL187.744 µL375.488 µL1.877 mL3.755 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.107
Extinction coefficient (cm -1 M -1)5900
Excitation (nm)336
Emission (nm)455

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)Correction Factor (260 nm)
ICG acid789813230000-0.076-
Cy3NS acid *CAS 1032678-01-5*55556915000010.1510.0730.07
Cy5NS acid65167025000010.271, 0.420.030.02
Cy7NS acid7567792500000.30.0360.05
AMCA acid *CAS#: 106562-32-7*34643419000-0.153-
EDANS iodoacetamide [5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid]3364555900-0.107-
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1*----0.516-
BCECF acid *CAS#: 85138-49-4*50452790000---
XFD532 acid *Same Structure to Alexa Fluor™ 532 acid*534553810000.6110.090.24
XFD488 acid *Same Structure to Alexa Fluor™ 488 acid*499520710000.9210.110.30
Cy3B acid56057112000010.5810.0690.048
XFD350 acid *Same Structure to Alexa Fluor™ 350 acid*34344119000-0.190.25
XFD546 acid *Same Structure to Alexa Fluor™ 546 acid*5615721120000.7910.120.21
XFD568 acid *Same Structure to Alexa Fluor™ 568 acid*579603913000.6910.460.45
XFD514 acid51854380000-0.180.31
QXY7 acid [equivalent to QSY-7 acid]--900001-0.22-
XFD555 acid5535681550000.110.080.08
XFD647 acid6506712700000.3310.03-
XFD750 acid7527762900000.1210.04-
XFD700 acid6967192050000.2510.07-
Show More (11)

References


View all 54 references: Citation Explorer
The beta4-beta8 Groove Is an ATP-interactive Site in the alpha Crystallin Core Domain of the Small Heat Shock Protein, Human alphaB Crystallin
Authors: Ghosh JG, Houck SA, Doneanu CE, Clark JI.
Journal: J Mol Biol (2006): 364
Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Characterization and inhibition of SARS-coronavirus main protease
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
Fluorogenic peptide substrates containing benzoxazol-5-yl-alanine derivatives for kinetic assay of cysteine proteases
Authors: Szabelski M, Rogiewicz M, Wiczk W.
Journal: Anal Biochem (2005): 20
ADAM33 enzyme properties and substrate specificity
Authors: Zou J, Zhang R, Zhu F, Liu J, Madison V, Uml and SP., undefined
Journal: Biochemistry (2005): 4247
Chemical synthesis of triple-labelled three-helix bundle binding proteins for specific fluorescent detection of unlabelled protein
Authors: Engfeldt T, Renberg B, Brumer H, Nygren PA, Karlstrom AE.
Journal: Chembiochem (2005): 1043
Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique
Authors: Chen S, Chen LL, Luo HB, Sun T, Chen J, Ye F, Cai JH, Shen JK, Shen X, Jiang HL.
Journal: Acta Pharmacol Sin (2005): 99
On the sequential determinants of calpain cleavage
Authors: Tompa P, Buzder-Lantos P, Tantos A, Farkas A, Szilagyi A, Banoczi Z, Hudecz F, Friedrich P.
Journal: J Biol Chem (2004): 20775