EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] CAS 50402-56-7

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Physical properties

Molecular weight	266.32
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.107
Extinction coefficient (cm ^-1 M ^-1)	5900
Excitation (nm)	336
Emission (nm)	455

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Alternative formats

EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]

Overview SDS Protocol

CAS

50402-56-7

Molecular weight

266.32

Correction Factor (280 nm)

0.107

Extinction coefficient (cm ^-1 M ^-1)

5900

Excitation (nm)

336

Emission (nm)

455

EDANS is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	375.488 µL	1.877 mL	3.755 mL	18.774 mL	37.549 mL
5 mM	75.098 µL	375.488 µL	750.976 µL	3.755 mL	7.51 mL
10 mM	37.549 µL	187.744 µL	375.488 µL	1.877 mL	3.755 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.107
Extinction coefficient (cm ^-1 M ^-1)	5900
Excitation (nm)	336
Emission (nm)	455

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)	Correction Factor (260 nm)
ICG acid	789	814	-	-	0.076	-
Cy3NS acid CAS 1032678-01-5	555	569	150000¹	0.15¹	0.073	0.07
Cy5NS acid	651	670	250000¹	0.27¹, 0.4²	0.03	0.02
Cy7NS acid	756	779	250000	0.3	0.036	0.05
AMCA acid CAS#: 106562-32-7	346	434	19000	-	0.153	-
EDANS iodoacetamide [5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid]	336	455	5900	-	0.107	-
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] CAS 6268-49-1	-	-	-	-	0.516	-
BCECF acid CAS#: 85138-49-4	504	527	90000	-	-	-
XFD532 acid Same Structure to Alexa Fluor™ 532 acid	317	553	81000	0.61¹	0.09	0.24
XFD488 acid Same Structure to Alexa Fluor™ 488 acid	490	520	71000	0.92¹	0.11	0.30
Cy3B acid	560	571	120000¹	0.58¹	0.069	0.048
XFD350 acid Same Structure to Alexa Fluor™ 350 acid	303	439	19000	-	0.19	0.25
XFD546 acid Same Structure to Alexa Fluor™ 546 acid	323	572	112000	0.79¹	0.12	0.21
XFD568 acid Same Structure to Alexa Fluor™ 568 acid	546	603	91300	0.69¹	0.46	0.45
XFD514 acid	315	543	80000	-	0.18	0.31
QXY7 acid [equivalent to QSY-7 NHS ester]	-	-	90000¹	-	0.22	-
XFD555 acid	553	568	155000	0.1¹	0.08	0.08
XFD647 acid	650	671	270000	0.33¹	0.03	-
XFD750 acid	752	776	290000	0.12¹	0.04	-
XFD700 acid	696	719	205000	0.25¹	0.07	-
Show More (11)

Images

Figure 1. Chemical structure for EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid] *CAS 50402-56-7*

Figure 2. Chemical structure for EDANS acid [5-((2-Aminoethyl)amino)naphthalene-1-sulfonic acid]

References

View all 54 references: Citation Explorer

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Journal: Protein Expr Purif (2006): 88

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Characterization and inhibition of SARS-coronavirus main protease
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ADAM33 enzyme properties and substrate specificity
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Journal: Biochemistry (2005): 4247

Chemical synthesis of triple-labelled three-helix bundle binding proteins for specific fluorescent detection of unlabelled protein
Authors: Engfeldt T, Renberg B, Brumer H, Nygren PA, Karlstrom AE.
Journal: Chembiochem (2005): 1043

Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique
Authors: Chen S, Chen LL, Luo HB, Sun T, Chen J, Ye F, Cai JH, Shen JK, Shen X, Jiang HL.
Journal: Acta Pharmacol Sin (2005): 99

On the sequential determinants of calpain cleavage
Authors: Tompa P, Buzder-Lantos P, Tantos A, Farkas A, Szilagyi A, Banoczi Z, Hudecz F, Friedrich P.
Journal: J Biol Chem (2004): 20775