DABCYL C2 maleimide

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	391.42
Solvent	DMSO

Spectral properties

Absorbance (nm)	454
Correction Factor (280 nm)	0.516

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Alternative formats

DABCYL C2 amine

Overview SDS Protocol

Molecular weight

391.42

Absorbance (nm)

454

Correction Factor (280 nm)

0.516

DABCYL C2 maleimide is the thiol-reactive form of DABCYL, and widely used to prepare a variety of FRET-based probes that contain DABCYL. DABCYL is one of the most popular acceptors for developing FRET-based nucleic acid probes and protease substrates. It is often paired with EDANS or a blue fluorescent donor such as coumarin dyes.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL C2 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	255.48 µL	1.277 mL	2.555 mL	12.774 mL	25.548 mL
5 mM	51.096 µL	255.48 µL	510.96 µL	2.555 mL	5.11 mL
10 mM	25.548 µL	127.74 µL	255.48 µL	1.277 mL	2.555 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	454
Correction Factor (280 nm)	0.516

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
AMCA C2 Maleimide	346	434	19000	0.153
EDANS C2 maleimide	336	455	5900	0.107

Images

Figure 1. Chemical structure for DABCYL C2 maleimide

Citations

View all 1 citations: Citation Explorer

Protein N-terminal processing: substrate specificity of Escherichia coli and human methionine aminopeptidases
Authors: Xiao, Qing and Zhang, Feiran and Nacev, Benjamin A and Liu, Jun O and Pei, Dehua
Journal: Biochemistry (2010): 5588--5599

References

View all 34 references: Citation Explorer

Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88

Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626

A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110

Characterization and inhibition of SARS-coronavirus main protease
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361

Fluorogenic peptide substrates containing benzoxazol-5-yl-alanine derivatives for kinetic assay of cysteine proteases
Authors: Szabelski M, Rogiewicz M, Wiczk W.
Journal: Anal Biochem (2005): 20

ADAM33 enzyme properties and substrate specificity
Authors: Zou J, Zhang R, Zhu F, Liu J, Madison V, Uml and SP., undefined
Journal: Biochemistry (2005): 4247

Enzymatic activity characterization of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer technique
Authors: Chen S, Chen LL, Luo HB, Sun T, Chen J, Ye F, Cai JH, Shen JK, Shen X, Jiang HL.
Journal: Acta Pharmacol Sin (2005): 99

Fluorogenic peptide substrates for carboxydipeptidase activity of cathepsin B
Authors: Stachowiak K, Tokmina M, Karpinska A, Sosnowska R, Wiczk W.
Journal: Acta Biochim Pol (2004): 81

Synthesis of poly(ethylene glycol)-based saquinavir prodrug conjugates and assessment of release and anti-HIV-1 bioactivity using a novel protease inhibition assay
Authors: Gunaseelan S, Debrah O, Wan L, Leibowitz MJ, Rabson AB, Stein S, Sinko PJ.
Journal: Bioconjug Chem (2004): 1322

Highly sensitive intramolecularly quenched fluorogenic substrates for renin based on the combination of L-2-amino-3-(7-methoxy-4-coumaryl)propionic acid with 2,4-dinitrophenyl groups at various positions
Authors: Paschalidou K, Neumann U, Gerhartz B, Tzougraki C.
Journal: Biochem J (2004): 1031