DABCYL-DBCO
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 527.63 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), PCR Detection of Viral DNA/RNA
Molecular weight 527.63 |
DABCYL is one of the most common dark quenchers used to prepare a variety of FRET-based nucleic acid probes and protease substrates. A dark quencher is a substance that absorbs excitation energy from a fluorophore and dissipates the energy as heat. When a dark quencher and a fluorophore are close together, such as in a molecule or protein, the fluorophore's emission is suppressed. This effect can be used to study molecular geometry and motion. DABCYL-DBCO is a convenient building block for developing FRET probes via the DBCO-based click chemistry, one of the most well-known strain-promoted azide-alkyne cycloadditions (SPAAC).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL-DBCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 189.527 µL | 947.634 µL | 1.895 mL | 9.476 mL | 18.953 mL |
5 mM | 37.905 µL | 189.527 µL | 379.054 µL | 1.895 mL | 3.791 mL |
10 mM | 18.953 µL | 94.763 µL | 189.527 µL | 947.634 µL | 1.895 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
DBCO-Cy3 | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
DBCO-Cy5 | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
DABCYL acid [4-((4-(Dimethylamino)phenyl)azo)benzoic acid] *CAS 6268-49-1* | - | - | - | - | - | 0.516 |
Cy3B DBCO | 560 | 571 | 1200001 | 0.581 | 0.048 | 0.069 |
References
View all 50 references: Citation Explorer
A Cell Membrane-Targeting Self-Delivery Chimeric Peptide for Enhanced Photodynamic Therapy and In Situ Therapeutic Feedback.
Authors: Ma, Wen and Sha, Sui-Nan and Chen, Pei-Ling and Yu, Meng and Chen, Jian-Jun and Huang, Chao-Bo and Yu, Bin and Liu, Yun and Liu, Li-Han and Yu, Zhi-Qiang
Journal: Advanced healthcare materials (2020): e1901100
Authors: Ma, Wen and Sha, Sui-Nan and Chen, Pei-Ling and Yu, Meng and Chen, Jian-Jun and Huang, Chao-Bo and Yu, Bin and Liu, Yun and Liu, Li-Han and Yu, Zhi-Qiang
Journal: Advanced healthcare materials (2020): e1901100
Polymeric fluorescent heparin as one-step FRET substrate of human heparanase.
Authors: Sistla, Jyothi C and Morla, Shravan and Alabbas, Al-Humaidi B and Kalathur, Ravi C and Sharon, Chetna and Patel, Bhaumik B and Desai, Umesh R
Journal: Carbohydrate polymers (2019): 385-391
Authors: Sistla, Jyothi C and Morla, Shravan and Alabbas, Al-Humaidi B and Kalathur, Ravi C and Sharon, Chetna and Patel, Bhaumik B and Desai, Umesh R
Journal: Carbohydrate polymers (2019): 385-391
FRET-Based Assays to Determine Calpain Activity.
Authors: McCartney, Christian-Scott E and Davies, Peter L
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 39-55
Authors: McCartney, Christian-Scott E and Davies, Peter L
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 39-55
Array-based in situ fluorescence assay for profiling multiplex matrix metalloproteinases activities in tissue section.
Authors: Lei, Zhen and Jian, Minghong and Wei, Jia and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin
Journal: Analytica chimica acta (2019): 112-118
Authors: Lei, Zhen and Jian, Minghong and Wei, Jia and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin
Journal: Analytica chimica acta (2019): 112-118
The conversion of azo-quenchers to fluorophores.
Authors: Hofstetter, O and Hofstetter, H and Miron, T and Wilchek, M
Journal: Analytical biochemistry (2019): 113400
Authors: Hofstetter, O and Hofstetter, H and Miron, T and Wilchek, M
Journal: Analytical biochemistry (2019): 113400
Development of a peptide substrate for detection of sunn pest damage in wheat flour.
Authors: Hançerlioğulları, Begüm Zeynep and Köksel, Hamit and Dudak, Fahriye Ceyda
Journal: Journal of the science of food and agriculture (2018): 5677-5682
Authors: Hançerlioğulları, Begüm Zeynep and Köksel, Hamit and Dudak, Fahriye Ceyda
Journal: Journal of the science of food and agriculture (2018): 5677-5682
A FRET-based dual-color evanescent wave optical fiber aptasensor for simultaneous fluorometric determination of aflatoxin M1 and ochratoxin A.
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Long, Feng
Journal: Mikrochimica acta (2018): 508
Authors: Song, Dan and Yang, Rong and Fang, Shunyan and Liu, Yanping and Long, Feng
Journal: Mikrochimica acta (2018): 508
Uleine Disrupts Key Enzymatic and Non-Enzymatic Biomarkers that Leads to Alzheimer's Disease.
Authors: Seidl, Claudia and de Moraes Santos, Cid Aimbire and De Simone, Angela and Bartolini, Manuela and Weffort-Santos, Almeriane Maria and Andrisano, Vincenza
Journal: Current Alzheimer research (2017): 317-326
Authors: Seidl, Claudia and de Moraes Santos, Cid Aimbire and De Simone, Angela and Bartolini, Manuela and Weffort-Santos, Almeriane Maria and Andrisano, Vincenza
Journal: Current Alzheimer research (2017): 317-326
Highly specific C-C bond cleavage induced FRET fluorescence for in vivo biological nitric oxide imaging.
Authors: Li, Hua and Zhang, Deliang and Gao, Mengna and Huang, Lumei and Tang, Longguang and Li, Zijing and Chen, Xiaoyuan and Zhang, Xianzhong
Journal: Chemical science (2017): 2199-2203
Authors: Li, Hua and Zhang, Deliang and Gao, Mengna and Huang, Lumei and Tang, Longguang and Li, Zijing and Chen, Xiaoyuan and Zhang, Xianzhong
Journal: Chemical science (2017): 2199-2203
Chemoenzymatic synthesis of fluorogenic phospholipids and evaluation in assays of phospholipases A, C and D.
Authors: Piel, Mathilde S and Peters, Günther H J and Brask, Jesper
Journal: Chemistry and physics of lipids (2017): 49-54
Authors: Piel, Mathilde S and Peters, Günther H J and Brask, Jesper
Journal: Chemistry and physics of lipids (2017): 49-54
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?