DABCYL-DBCO

DABCYL is one of the most common dark quenchers used to prepare a variety of FRET-based nucleic acid probes and protease substrates. A dark quencher is a substance that absorbs excitation energy from a fluorophore and dissipates the energy as heat. When a dark quencher and a fluorophore are close together, such as in a molecule or protein, the fluorophore's emission is suppressed. This effect can be used to study molecular geometry and motion. DABCYL-DBCO is a convenient building block for developing FRET probes via the DBCO-based click chemistry, one of the most well-known strain-promoted azide-alkyne cycloadditions (SPAAC).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2010	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL-DBCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	189.527 µL	947.634 µL	1.895 mL	9.476 mL	18.953 mL
5 mM	37.905 µL	189.527 µL	379.054 µL	1.895 mL	3.791 mL
10 mM	18.953 µL	94.763 µL	189.527 µL	947.634 µL	1.895 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	527.63 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

A Cell Membrane-Targeting Self-Delivery Chimeric Peptide for Enhanced Photodynamic Therapy and In Situ Therapeutic Feedback.

Authors:

Ma, Wen and Sha, Sui-Nan and Chen, Pei-Ling and Yu, Meng and Chen, Jian-Jun and Huang, Chao-Bo and Yu, Bin and Liu, Yun and Liu, Li-Han and Yu, Zhi-Qiang

Journal:

Advanced healthcare materials (2020): e1901100

Polymeric fluorescent heparin as one-step FRET substrate of human heparanase.

Authors:

Sistla, Jyothi C and Morla, Shravan and Alabbas, Al-Humaidi B and Kalathur, Ravi C and Sharon, Chetna and Patel, Bhaumik B and Desai, Umesh R

Journal:

Carbohydrate polymers (2019): 385-391

FRET-Based Assays to Determine Calpain Activity.

Authors:

McCartney, Christian-Scott E and Davies, Peter L

Journal:

Methods in molecular biology (Clifton, N.J.) (2019): 39-55

Array-based in situ fluorescence assay for profiling multiplex matrix metalloproteinases activities in tissue section.

Authors:

Lei, Zhen and Jian, Minghong and Wei, Jia and Wang, Yaoqi and Meng, Xianying and Wang, Zhenxin

Journal:

Analytica chimica acta (2019): 112-118

The conversion of azo-quenchers to fluorophores.

Authors:

Hofstetter, O and Hofstetter, H and Miron, T and Wilchek, M

Journal:

Analytical biochemistry (2019): 113400

Physical properties

Molecular weight	527.63
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 23, 2025