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DABCYL succinimidyl ester [4-((4-(Dimethylamino)phenyl)azo)benzoic acid, succinimidyl ester] *CAS 146998-31-4*

Methionine aminopeptidase (MetAP) catalyzes the hydrolytic cleavage of the N-terminal methionine from newly synthesized polypeptides. The extent of removal of methionyl from a protein is dictated by its N-terminal peptide sequence. Earlier studies revealed that MetAPs require amino acids containing small side chains (e.g., Gly, Ala, Ser, Cys, Pro, Thr, and Val) as the P1′ residue, but their specificity at positions P2′ and beyond remains incompletely defined. In this work, the substrate specificities of <em>Escherichia coli</em> MetAP1, human MetAP1, and human MetAP2 were systematically profiled by screening against a combinatorial peptide library and kinetic analysis of individually synthesized peptide substrates. Our results show that although all three enzymes require small residues at the P1′ position, they have differential tolerance for Val and Thr at this position. The catalytic activity of human MetAP2 toward Met-Val peptides is consistently 2 orders of magnitude higher than that of MetAP1, suggesting that MetAP2 is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences <em>in vivo</em>. At positions P2′−P5′, all three MetAPs have broad specificity but are poorly active toward peptides containing a proline at the P2′ position. In addition, the human MetAPs disfavor acidic residues at the P2′−P5′ positions. The specificity data have allowed us to formulate a simple set of rules that can reliably predict the N-terminal processing of <em>E. coli</em> and human proteins. Source: <strong>Protein N-Terminal Processing: Substrate Specificity of Escherichia coli and Human Methionine Aminopeptidases</strong> by Xiao et al., <em>ACS Publications</em>, June 2010.
Methionine aminopeptidase (MetAP) catalyzes the hydrolytic cleavage of the N-terminal methionine from newly synthesized polypeptides. The extent of removal of methionyl from a protein is dictated by its N-terminal peptide sequence. Earlier studies revealed that MetAPs require amino acids containing small side chains (e.g., Gly, Ala, Ser, Cys, Pro, Thr, and Val) as the P1′ residue, but their specificity at positions P2′ and beyond remains incompletely defined. In this work, the substrate specificities of <em>Escherichia coli</em> MetAP1, human MetAP1, and human MetAP2 were systematically profiled by screening against a combinatorial peptide library and kinetic analysis of individually synthesized peptide substrates. Our results show that although all three enzymes require small residues at the P1′ position, they have differential tolerance for Val and Thr at this position. The catalytic activity of human MetAP2 toward Met-Val peptides is consistently 2 orders of magnitude higher than that of MetAP1, suggesting that MetAP2 is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences <em>in vivo</em>. At positions P2′−P5′, all three MetAPs have broad specificity but are poorly active toward peptides containing a proline at the P2′ position. In addition, the human MetAPs disfavor acidic residues at the P2′−P5′ positions. The specificity data have allowed us to formulate a simple set of rules that can reliably predict the N-terminal processing of <em>E. coli</em> and human proteins. Source: <strong>Protein N-Terminal Processing: Substrate Specificity of Escherichia coli and Human Methionine Aminopeptidases</strong> by Xiao et al., <em>ACS Publications</em>, June 2010.
Chemical structure for DABCYL succinimidyl ester [4-((4-(Dimethylamino)phenyl)azo)benzoic acid, succinimidyl ester] *CAS 146998-31-4*
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Catalog Number2004
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Physical properties
Molecular weight366.37
SolventDMSO
Spectral properties
Absorbance (nm)454
Correction Factor (280 nm)0.516
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

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CAS
146998-31-4
Molecular weight
366.37
Absorbance (nm)
454
Correction Factor (280 nm)
0.516
DABCYL, SE is the amino-reactive form of DABCYL, and widely used to prepare a variety of FRET-based probes that contain DABCYL. DABCYL is one of the most popular acceptors for developing FRET-based nucleic acid probes and protease substrates.

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Table 1. Volume of DMSO needed to reconstitute specific mass of DABCYL succinimidyl ester [4-((4-(Dimethylamino)phenyl)azo)benzoic acid, succinimidyl ester] *CAS 146998-31-4* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM272.948 µL1.365 mL2.729 mL13.647 mL27.295 mL
5 mM54.59 µL272.948 µL545.896 µL2.729 mL5.459 mL
10 mM27.295 µL136.474 µL272.948 µL1.365 mL2.729 mL

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Spectral properties

Absorbance (nm)454
Correction Factor (280 nm)0.516

Citations


View all 1 citations: Citation Explorer
Protein N-terminal processing: substrate specificity of Escherichia coli and human methionine aminopeptidases
Authors: Xiao, Qing and Zhang, Feiran and Nacev, Benjamin A and Liu, Jun O and Pei, Dehua
Journal: Biochemistry (2010): 5588--5599

References


View all 34 references: Citation Explorer
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Fluorogenic peptide substrates containing benzoxazol-5-yl-alanine derivatives for kinetic assay of cysteine proteases
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ADAM33 enzyme properties and substrate specificity
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Journal: Biochemistry (2005): 4247
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Synthesis of poly(ethylene glycol)-based saquinavir prodrug conjugates and assessment of release and anti-HIV-1 bioactivity using a novel protease inhibition assay
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Highly sensitive intramolecularly quenched fluorogenic substrates for renin based on the combination of L-2-amino-3-(7-methoxy-4-coumaryl)propionic acid with 2,4-dinitrophenyl groups at various positions
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Journal: Biochem J (2004): 1031