DAF-2 | AAT Bioquest

DAF-2

The weakly fluorescent DAF-2 is a sensitive fluorogenic indicator commonly used for detecting nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein. Its fluorescence can be monitored using the standard FITC filter set. At neutral pH the detection limit is 2-5 nM for NO. It might be used for real-time NO detection, but the optimal DAF-2 concentration must be experimentally determined for a cellular NO detection. For example, a low DAF-2 concentration (e.g., 0.1 μM) was used for the real-time detection of endothelial NO formation by subtracting the DAF-2 auto-fluorescence from the measured total fluorescence.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
16290	1 mg	Price

References

View all 50 references: Citation Explorer

Involvement of nitrate reductase-dependent nitric oxide production in magnetopriming-induced salt tolerance in soybean.

Authors:

Kataria, Sunita and Jain, Meeta and Tripathi, Durgesh K and Singh, Vijay P

Journal:

Physiologia plantarum (2020): 422-436

Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation?

Authors:

Miguel-Jiménez, Sara and Carvajal-Serna, Melissa and Calvo, Silvia and Casao, Adriana and Cebrián-Pérez, José Álvaro and Muiño-Blanco, Teresa and Pérez-Pe, Rosaura

Journal:

International journal of molecular sciences (2020)

Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction.

Authors:

de Oliveira, Suelen Guedes and Claudio, Erick Roberto Gonçalves and de Almeida, Simone Alves and Mengal, Vinicius and da Silva, Fabricio Bragança and Silva, Nyam Florêncio and Mauad, Helder and de Abreu, Glaucia Rodrigues

Journal:

PloS one (2019): e0215568

Extracellular and Intracellular NO Detection in Plants by Diaminofluoresceins.

Authors:

Silveira, Neidiquele Maria and Machado, Eduardo Caruso and Ribeiro, Rafael Vasconcelos

Journal:

Methods in molecular biology (Clifton, N.J.) (2019): 103-108

Curcumin prevents strokes in stroke-prone spontaneously hypertensive rats by improving vascular endothelial function.

Authors:

Lan, Cong and Chen, Xinjian and Zhang, Yuxun and Wang, Wei and Wang, Wei Eric and Liu, Yukai and Cai, Yue and Ren, Hongmei and Zheng, Shuo and Zhou, Lin and Zeng, Chunyu

Journal:

BMC cardiovascular disorders (2018): 43

Physical properties

Molecular weight	362.34
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	205391-01-1

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Page updated on October 8, 2025