AAT Bioquest


Product Image
Product Image
Gallery Image 1
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight362.34
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
The weakly fluorescent DAF-2 is a sensitive fluorogenic indicator commonly used for detecting nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein. Its fluorescence can be monitored using the standard FITC filter set. At neutral pH the detection limit is 2-5 nM for NO. It might be used for real-time NO detection, but the optimal DAF-2 concentration must be experimentally determined for a cellular NO detection. For example, a low DAF-2 concentration (e.g., 0.1 μM) was used for the real-time detection of endothelial NO formation by subtracting the DAF-2 auto-fluorescence from the measured total fluorescence.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DAF-2 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM275.984 µL1.38 mL2.76 mL13.799 mL27.598 mL
5 mM55.197 µL275.984 µL551.968 µL2.76 mL5.52 mL
10 mM27.598 µL137.992 µL275.984 µL1.38 mL2.76 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



View all 50 references: Citation Explorer
Involvement of nitrate reductase-dependent nitric oxide production in magnetopriming-induced salt tolerance in soybean.
Authors: Kataria, Sunita and Jain, Meeta and Tripathi, Durgesh K and Singh, Vijay P
Journal: Physiologia plantarum (2020): 422-436
Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation?
Authors: Miguel-Jiménez, Sara and Carvajal-Serna, Melissa and Calvo, Silvia and Casao, Adriana and Cebrián-Pérez, José Álvaro and Muiño-Blanco, Teresa and Pérez-Pe, Rosaura
Journal: International journal of molecular sciences (2020)
Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction.
Authors: de Oliveira, Suelen Guedes and Claudio, Erick Roberto Gonçalves and de Almeida, Simone Alves and Mengal, Vinicius and da Silva, Fabricio Bragança and Silva, Nyam Florêncio and Mauad, Helder and de Abreu, Glaucia Rodrigues
Journal: PloS one (2019): e0215568
Extracellular and Intracellular NO Detection in Plants by Diaminofluoresceins.
Authors: Silveira, Neidiquele Maria and Machado, Eduardo Caruso and Ribeiro, Rafael Vasconcelos
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 103-108
Curcumin prevents strokes in stroke-prone spontaneously hypertensive rats by improving vascular endothelial function.
Authors: Lan, Cong and Chen, Xinjian and Zhang, Yuxun and Wang, Wei and Wang, Wei Eric and Liu, Yukai and Cai, Yue and Ren, Hongmei and Zheng, Shuo and Zhou, Lin and Zeng, Chunyu
Journal: BMC cardiovascular disorders (2018): 43
Nitric oxide in frozen-thawed equine sperm: Effects on motility, membrane integrity and sperm capacitation.
Authors: de Andrade, André F C and Arruda, Rubens P and Torres, Mariana A and Pieri, Naira C G and Leite, Ticiano G and Celeghini, Eneiva Carla C and Oliveira, Leticia Z and Gardés, Thayna P and Bussiere, Maria Clara C and Silva, Daniela F
Journal: Animal reproduction science (2018): 176-184
Nandrolone combined with strenuous resistance training reduces vascular nitric oxide bioavailability and impairs endothelium-dependent vasodilation.
Authors: Guzzoni, Vinicius and Cunha, Tatiana Sousa and das Neves, Vander José and Briet, Larissa and Costa, Rafaela and Moura, Maria José Costa Sampaio and Oliveira, Vanessa and Franco, Maria do Carmo Pinho and Novaes, Pedro Duarte and Marcondes, Fernanda Klein
Journal: Steroids (2018): 7-13
Inter- and intra-subject variability of nitric oxide levels in leukocyte subpopulations.
Authors: Maharaj, Sheena and Lu, Kim D and Radom-Aizik, Shlomit and Zaldivar, Frank and Haddad, Fadia and Shin, Hye-Won and Leu, Szu-Yun and Nussbaum, Eliezer and Randhawa, Inderpal and Cooper, Dan M
Journal: Nitric oxide : biology and chemistry (2018): 41-45
Nitric oxide-coupled signaling in odor elicited molecular events in the olfactory center of the terrestrial snail, Helix pomatia.
Authors: Serfőző, Zoltán and Nacsa, Kálmán and Veréb, Zoltán and Battonyai, Izabella and Hegedűs, Csaba and Balogh, Csilla and Elekes, Károly
Journal: Cellular signalling (2017): 67-81
Donepezil suppresses intracellular Ca2+ mobilization through the PI3K pathway in rodent microglia.
Authors: Haraguchi, Yoshinori and Mizoguchi, Yoshito and Ohgidani, Masahiro and Imamura, Yoshiomi and Murakawa-Hirachi, Toru and Nabeta, Hiromi and Tateishi, Hiroshi and Kato, Takahiro A and Monji, Akira
Journal: Journal of neuroinflammation (2017): 258