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DAF-2
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The weakly fluorescent DAF-2 is a sensitive fluorogenic indicator commonly used for detecting nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein. Its fluorescence can be monitored using the standard FITC filter set. At neutral pH the detection limit is 2-5 nM for NO. It might be used for real-time NO detection, but the optimal DAF-2 concentration must be experimentally determined for a cellular NO detection. For example, a low DAF-2 concentration (e.g., 0.1 μM) was used for the real-time detection of endothelial NO formation by subtracting the DAF-2 auto-fluorescence from the measured total fluorescence.
Product family
| Name | Excitation (nm) | Emission (nm) |
| 2-Aminoacridone *CAS 27918-14-5* | 429 | 529 |
| BTA-2 [2-(4'-(dimethylamino)phenyl)-6-methyl-benzothiazole] | 355 | 426 |
| 2-NBDG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose] *CAS#: 186689-07-6* | 467 | 538 |
References
View all 50 references: Citation Explorer
Involvement of nitrate reductase-dependent nitric oxide production in magnetopriming-induced salt tolerance in soybean.
Authors: Kataria, Sunita and Jain, Meeta and Tripathi, Durgesh K and Singh, Vijay P
Journal: Physiologia plantarum (2020): 422-436
Authors: Kataria, Sunita and Jain, Meeta and Tripathi, Durgesh K and Singh, Vijay P
Journal: Physiologia plantarum (2020): 422-436
Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation?
Authors: Miguel-Jiménez, Sara and Carvajal-Serna, Melissa and Calvo, Silvia and Casao, Adriana and Cebrián-Pérez, José Álvaro and Muiño-Blanco, Teresa and Pérez-Pe, Rosaura
Journal: International journal of molecular sciences (2020)
Authors: Miguel-Jiménez, Sara and Carvajal-Serna, Melissa and Calvo, Silvia and Casao, Adriana and Cebrián-Pérez, José Álvaro and Muiño-Blanco, Teresa and Pérez-Pe, Rosaura
Journal: International journal of molecular sciences (2020)
Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction.
Authors: de Oliveira, Suelen Guedes and Claudio, Erick Roberto Gonçalves and de Almeida, Simone Alves and Mengal, Vinicius and da Silva, Fabricio Bragança and Silva, Nyam Florêncio and Mauad, Helder and de Abreu, Glaucia Rodrigues
Journal: PloS one (2019): e0215568
Authors: de Oliveira, Suelen Guedes and Claudio, Erick Roberto Gonçalves and de Almeida, Simone Alves and Mengal, Vinicius and da Silva, Fabricio Bragança and Silva, Nyam Florêncio and Mauad, Helder and de Abreu, Glaucia Rodrigues
Journal: PloS one (2019): e0215568
Extracellular and Intracellular NO Detection in Plants by Diaminofluoresceins.
Authors: Silveira, Neidiquele Maria and Machado, Eduardo Caruso and Ribeiro, Rafael Vasconcelos
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 103-108
Authors: Silveira, Neidiquele Maria and Machado, Eduardo Caruso and Ribeiro, Rafael Vasconcelos
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 103-108
Curcumin prevents strokes in stroke-prone spontaneously hypertensive rats by improving vascular endothelial function.
Authors: Lan, Cong and Chen, Xinjian and Zhang, Yuxun and Wang, Wei and Wang, Wei Eric and Liu, Yukai and Cai, Yue and Ren, Hongmei and Zheng, Shuo and Zhou, Lin and Zeng, Chunyu
Journal: BMC cardiovascular disorders (2018): 43
Authors: Lan, Cong and Chen, Xinjian and Zhang, Yuxun and Wang, Wei and Wang, Wei Eric and Liu, Yukai and Cai, Yue and Ren, Hongmei and Zheng, Shuo and Zhou, Lin and Zeng, Chunyu
Journal: BMC cardiovascular disorders (2018): 43