DAF-2
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 362.34 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Reactive Oxygen Species (ROS)
CAS 205391-01-1 | Molecular weight 362.34 |
The weakly fluorescent DAF-2 is a sensitive fluorogenic indicator commonly used for detecting nitric oxide (NO). It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein. Its fluorescence can be monitored using the standard FITC filter set. At neutral pH the detection limit is 2-5 nM for NO. It might be used for real-time NO detection, but the optimal DAF-2 concentration must be experimentally determined for a cellular NO detection. For example, a low DAF-2 concentration (e.g., 0.1 μM) was used for the real-time detection of endothelial NO formation by subtracting the DAF-2 auto-fluorescence from the measured total fluorescence.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DAF-2 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 275.984 µL | 1.38 mL | 2.76 mL | 13.799 mL | 27.598 mL |
5 mM | 55.197 µL | 275.984 µL | 551.968 µL | 2.76 mL | 5.52 mL |
10 mM | 27.598 µL | 137.992 µL | 275.984 µL | 1.38 mL | 2.76 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) |
2-Aminoacridone *CAS 27918-14-5* | 429 | 529 |
BTA-2 [2-(4'-(dimethylamino)phenyl)-6-methyl-benzothiazole] | 355 | 426 |
2-NBDG [2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose] *CAS#: 186689-07-6* | 467 | 538 |
References
View all 50 references: Citation Explorer
Involvement of nitrate reductase-dependent nitric oxide production in magnetopriming-induced salt tolerance in soybean.
Authors: Kataria, Sunita and Jain, Meeta and Tripathi, Durgesh K and Singh, Vijay P
Journal: Physiologia plantarum (2020): 422-436
Authors: Kataria, Sunita and Jain, Meeta and Tripathi, Durgesh K and Singh, Vijay P
Journal: Physiologia plantarum (2020): 422-436
Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation?
Authors: Miguel-Jiménez, Sara and Carvajal-Serna, Melissa and Calvo, Silvia and Casao, Adriana and Cebrián-Pérez, José Álvaro and Muiño-Blanco, Teresa and Pérez-Pe, Rosaura
Journal: International journal of molecular sciences (2020)
Authors: Miguel-Jiménez, Sara and Carvajal-Serna, Melissa and Calvo, Silvia and Casao, Adriana and Cebrián-Pérez, José Álvaro and Muiño-Blanco, Teresa and Pérez-Pe, Rosaura
Journal: International journal of molecular sciences (2020)
Exercise training improves vascular reactivity in ovariectomized rats subjected to myocardial infarction.
Authors: de Oliveira, Suelen Guedes and Claudio, Erick Roberto Gonçalves and de Almeida, Simone Alves and Mengal, Vinicius and da Silva, Fabricio Bragança and Silva, Nyam Florêncio and Mauad, Helder and de Abreu, Glaucia Rodrigues
Journal: PloS one (2019): e0215568
Authors: de Oliveira, Suelen Guedes and Claudio, Erick Roberto Gonçalves and de Almeida, Simone Alves and Mengal, Vinicius and da Silva, Fabricio Bragança and Silva, Nyam Florêncio and Mauad, Helder and de Abreu, Glaucia Rodrigues
Journal: PloS one (2019): e0215568
Extracellular and Intracellular NO Detection in Plants by Diaminofluoresceins.
Authors: Silveira, Neidiquele Maria and Machado, Eduardo Caruso and Ribeiro, Rafael Vasconcelos
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 103-108
Authors: Silveira, Neidiquele Maria and Machado, Eduardo Caruso and Ribeiro, Rafael Vasconcelos
Journal: Methods in molecular biology (Clifton, N.J.) (2019): 103-108
Curcumin prevents strokes in stroke-prone spontaneously hypertensive rats by improving vascular endothelial function.
Authors: Lan, Cong and Chen, Xinjian and Zhang, Yuxun and Wang, Wei and Wang, Wei Eric and Liu, Yukai and Cai, Yue and Ren, Hongmei and Zheng, Shuo and Zhou, Lin and Zeng, Chunyu
Journal: BMC cardiovascular disorders (2018): 43
Authors: Lan, Cong and Chen, Xinjian and Zhang, Yuxun and Wang, Wei and Wang, Wei Eric and Liu, Yukai and Cai, Yue and Ren, Hongmei and Zheng, Shuo and Zhou, Lin and Zeng, Chunyu
Journal: BMC cardiovascular disorders (2018): 43
Nitric oxide in frozen-thawed equine sperm: Effects on motility, membrane integrity and sperm capacitation.
Authors: de Andrade, André F C and Arruda, Rubens P and Torres, Mariana A and Pieri, Naira C G and Leite, Ticiano G and Celeghini, Eneiva Carla C and Oliveira, Leticia Z and Gardés, Thayna P and Bussiere, Maria Clara C and Silva, Daniela F
Journal: Animal reproduction science (2018): 176-184
Authors: de Andrade, André F C and Arruda, Rubens P and Torres, Mariana A and Pieri, Naira C G and Leite, Ticiano G and Celeghini, Eneiva Carla C and Oliveira, Leticia Z and Gardés, Thayna P and Bussiere, Maria Clara C and Silva, Daniela F
Journal: Animal reproduction science (2018): 176-184
Nandrolone combined with strenuous resistance training reduces vascular nitric oxide bioavailability and impairs endothelium-dependent vasodilation.
Authors: Guzzoni, Vinicius and Cunha, Tatiana Sousa and das Neves, Vander José and Briet, Larissa and Costa, Rafaela and Moura, Maria José Costa Sampaio and Oliveira, Vanessa and Franco, Maria do Carmo Pinho and Novaes, Pedro Duarte and Marcondes, Fernanda Klein
Journal: Steroids (2018): 7-13
Authors: Guzzoni, Vinicius and Cunha, Tatiana Sousa and das Neves, Vander José and Briet, Larissa and Costa, Rafaela and Moura, Maria José Costa Sampaio and Oliveira, Vanessa and Franco, Maria do Carmo Pinho and Novaes, Pedro Duarte and Marcondes, Fernanda Klein
Journal: Steroids (2018): 7-13
Inter- and intra-subject variability of nitric oxide levels in leukocyte subpopulations.
Authors: Maharaj, Sheena and Lu, Kim D and Radom-Aizik, Shlomit and Zaldivar, Frank and Haddad, Fadia and Shin, Hye-Won and Leu, Szu-Yun and Nussbaum, Eliezer and Randhawa, Inderpal and Cooper, Dan M
Journal: Nitric oxide : biology and chemistry (2018): 41-45
Authors: Maharaj, Sheena and Lu, Kim D and Radom-Aizik, Shlomit and Zaldivar, Frank and Haddad, Fadia and Shin, Hye-Won and Leu, Szu-Yun and Nussbaum, Eliezer and Randhawa, Inderpal and Cooper, Dan M
Journal: Nitric oxide : biology and chemistry (2018): 41-45
Bacillus subtilis biofilm extends Caenorhabditis elegans longevity through downregulation of the insulin-like signalling pathway.
Authors: Donato, Verónica and Ayala, Facundo Rodríguez and Cogliati, Sebastián and Bauman, Carlos and Costa, Juan Gabriel and Leñini, Cecilia and Grau, Roberto
Journal: Nature communications (2017): 14332
Authors: Donato, Verónica and Ayala, Facundo Rodríguez and Cogliati, Sebastián and Bauman, Carlos and Costa, Juan Gabriel and Leñini, Cecilia and Grau, Roberto
Journal: Nature communications (2017): 14332
Chronic iron overload induces functional and structural vascular changes in small resistance arteries via NADPH oxidase-dependent O2- production.
Authors: Ribeiro Júnior, Rogério Faustino and Marques, Vinicius Bermond and Nunes, Dieli Oliveira and Stefanon, Ivanita and Dos Santos, Leonardo
Journal: Toxicology letters (2017): 43-52
Authors: Ribeiro Júnior, Rogério Faustino and Marques, Vinicius Bermond and Nunes, Dieli Oliveira and Stefanon, Ivanita and Dos Santos, Leonardo
Journal: Toxicology letters (2017): 43-52
Application notes
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Evaluation of FLIPR Calcium Assays for Screening GPCR and Calcium Channel Targets
Fluorescent Dye AM Esters
FAQ
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How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
What is the best way to stain cells with Membrane Marker 1-43 (MM 1-43)?