AAT Bioquest


Product Image
Product Image
Gallery Image 1
Polarization of hMDMs induces changes in gene expression and cell surface marker expression. M2 hMDMs had significantly lower intracellular nitric oxide in comparison with M0 hMDMs, paralleling gene expression changes. Intracellular NO production was measured using the NO reactive dye DAF-2 DA. Source: <b>Expanded characterization of <em>in vitro</em> polarized M0, M1, and M2 human monocyte-derived macrophages: Bioenergetic and secreted mediator profiles</b> by Hickman <em>et.al.</em>, <em>Bioenergetic and secreted mediator profiles</em>. PLoS ONE 18(3): e0279037. March 2023.
Ordering information
Catalog Number
Unit Size
Add to cart
Additional ordering information
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight446.42
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
DAF-2 diacetate is a more cell-permeable version of DAF-2, a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO). Upon entry into cells, DAF-2 diacetate is hydrolyzed to the less cell-permeable DAF-2 by cellular esterases, thus preventing loss of signal due to leakage of DAF-2 diacetate from cells. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein. The intracellular fluorescence (generated by DAF-2 diacetate) can be readily monitored using the standard FITC filter set. DAF-2 diacetate can be utilized in cells which produce small amounts of NO, such as endothelial cells, as well as in cells which generate large amounts of NO, such as macrophages.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DAF-2 DA to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM224.004 µL1.12 mL2.24 mL11.2 mL22.4 mL
5 mM44.801 µL224.004 µL448.009 µL2.24 mL4.48 mL
10 mM22.4 µL112.002 µL224.004 µL1.12 mL2.24 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



View all 1 citations: Citation Explorer
Expanded characterization of in vitro polarized M0, M1, and M2 human monocyte-derived macrophages: Bioenergetic and secreted mediator profiles
Authors: Hickman, Elise and Smyth, Timothy and Cobos-Uribe, Catalina and Immormino, Robert and Rebuli, Meghan E and Moran, Timothy and Alexis, Neil E and Jaspers, Ilona
Journal: Plos one (2023): e0279037


View all 50 references: Citation Explorer
Involvement of nitrate reductase-dependent nitric oxide production in magnetopriming-induced salt tolerance in soybean.
Authors: Kataria, Sunita and Jain, Meeta and Tripathi, Durgesh K and Singh, Vijay P
Journal: Physiologia plantarum (2020): 422-436
Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation?
Authors: Miguel-Jiménez, Sara and Carvajal-Serna, Melissa and Calvo, Silvia and Casao, Adriana and Cebrián-Pérez, José Álvaro and Muiño-Blanco, Teresa and Pérez-Pe, Rosaura
Journal: International journal of molecular sciences (2020)
Curcumin prevents strokes in stroke-prone spontaneously hypertensive rats by improving vascular endothelial function.
Authors: Lan, Cong and Chen, Xinjian and Zhang, Yuxun and Wang, Wei and Wang, Wei Eric and Liu, Yukai and Cai, Yue and Ren, Hongmei and Zheng, Shuo and Zhou, Lin and Zeng, Chunyu
Journal: BMC cardiovascular disorders (2018): 43
Nitric oxide in frozen-thawed equine sperm: Effects on motility, membrane integrity and sperm capacitation.
Authors: de Andrade, André F C and Arruda, Rubens P and Torres, Mariana A and Pieri, Naira C G and Leite, Ticiano G and Celeghini, Eneiva Carla C and Oliveira, Leticia Z and Gardés, Thayna P and Bussiere, Maria Clara C and Silva, Daniela F
Journal: Animal reproduction science (2018): 176-184
Inhibition of endothelial nitric oxide synthase decreases breast cancer cell MDA-MB-231 adhesion to intact microvessels under physiological flows.
Authors: Zhang, Lin and Zeng, Min and Fu, Bingmei M
Journal: American journal of physiology. Heart and circulatory physiology (2016): H1735-47
Oxidative Stress-Dependent Coronary Endothelial Dysfunction in Obese Mice.
Authors: Gamez-Mendez, Ana María and Vargas-Robles, Hilda and Ríos, Amelia and Escalante, Bruno
Journal: PloS one (2015): e0138609
Inhibition of autophagy by chloroquine stimulates nitric oxide production and protects endothelial function during serum deprivation.
Authors: Pestana, Cezar Rangel and Oishi, Jorge Camargo and Salistre-Araújo, Heloísa Sobreiro and Rodrigues, Gerson Jhonatan
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2015): 1168-77
Traumatic brain injury disrupts cerebrovascular tone through endothelial inducible nitric oxide synthase expression and nitric oxide gain of function.
Authors: Villalba, Nuria and Sonkusare, Swapnil K and Longden, Thomas A and Tran, Tram L and Sackheim, Adrian M and Nelson, Mark T and Wellman, George C and Freeman, Kalev
Journal: Journal of the American Heart Association (2014): e001474
Nrf2 activation supports cell survival during hypoxia and hypoxia/reoxygenation in cardiomyoblasts; the roles of reactive oxygen and nitrogen species.
Authors: Kolamunne, Rajitha T and Dias, Irundika H K and Vernallis, Ann B and Grant, Melissa M and Griffiths, Helen R
Journal: Redox biology (2013): 418-26
Nelfinavir suppresses insulin signaling and nitric oxide production by human aortic endothelial cells: protective effects of thiazolidinediones.
Authors: Mondal, Debasis and Liu, Kai and Hamblin, Milton and Lasky, Joseph A and Agrawal, Krishna C
Journal: The Ochsner journal (2013): 76-90