DAF-2 DA

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Telephone	1-800-990-8053
Fax	1-800-609-2943
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Physical properties

Molecular weight	446.42
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DAF-2 DA to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	224.004 µL	1.12 mL	2.24 mL	11.2 mL	22.4 mL
5 mM	44.801 µL	224.004 µL	448.009 µL	2.24 mL	4.48 mL
10 mM	22.4 µL	112.002 µL	224.004 µL	1.12 mL	2.24 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for DAF-2 DA.

Figure 2. Polarization of hMDMs induces changes in gene expression and cell surface marker expression. M2 hMDMs had significantly lower intracellular nitric oxide in comparison with M0 hMDMs, paralleling gene expression changes. Intracellular NO production was measured using the NO reactive dye DAF-2 DA. Source: Expanded characterization of in vitro polarized M0, M1, and M2 human monocyte-derived macrophages: Bioenergetic and secreted mediator profiles by Hickman et.al., Bioenergetic and secreted mediator profiles. PLoS ONE 18(3): e0279037. March 2023.

Citations

View all 1 citations: Citation Explorer

Expanded characterization of in vitro polarized M0, M1, and M2 human monocyte-derived macrophages: Bioenergetic and secreted mediator profiles
Authors: Hickman, Elise and Smyth, Timothy and Cobos-Uribe, Catalina and Immormino, Robert and Rebuli, Meghan E and Moran, Timothy and Alexis, Neil E and Jaspers, Ilona
Journal: Plos one (2023): e0279037

References

View all 50 references: Citation Explorer

Involvement of nitrate reductase-dependent nitric oxide production in magnetopriming-induced salt tolerance in soybean.
Authors: Kataria, Sunita and Jain, Meeta and Tripathi, Durgesh K and Singh, Vijay P
Journal: Physiologia plantarum (2020): 422-436

Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation?
Authors: Miguel-Jiménez, Sara and Carvajal-Serna, Melissa and Calvo, Silvia and Casao, Adriana and Cebrián-Pérez, José Álvaro and Muiño-Blanco, Teresa and Pérez-Pe, Rosaura
Journal: International journal of molecular sciences (2020)

Curcumin prevents strokes in stroke-prone spontaneously hypertensive rats by improving vascular endothelial function.
Authors: Lan, Cong and Chen, Xinjian and Zhang, Yuxun and Wang, Wei and Wang, Wei Eric and Liu, Yukai and Cai, Yue and Ren, Hongmei and Zheng, Shuo and Zhou, Lin and Zeng, Chunyu
Journal: BMC cardiovascular disorders (2018): 43

Nitric oxide in frozen-thawed equine sperm: Effects on motility, membrane integrity and sperm capacitation.
Authors: de Andrade, André F C and Arruda, Rubens P and Torres, Mariana A and Pieri, Naira C G and Leite, Ticiano G and Celeghini, Eneiva Carla C and Oliveira, Leticia Z and Gardés, Thayna P and Bussiere, Maria Clara C and Silva, Daniela F
Journal: Animal reproduction science (2018): 176-184

Inhibition of endothelial nitric oxide synthase decreases breast cancer cell MDA-MB-231 adhesion to intact microvessels under physiological flows.
Authors: Zhang, Lin and Zeng, Min and Fu, Bingmei M
Journal: American journal of physiology. Heart and circulatory physiology (2016): H1735-47

Oxidative Stress-Dependent Coronary Endothelial Dysfunction in Obese Mice.
Authors: Gamez-Mendez, Ana María and Vargas-Robles, Hilda and Ríos, Amelia and Escalante, Bruno
Journal: PloS one (2015): e0138609

Inhibition of autophagy by chloroquine stimulates nitric oxide production and protects endothelial function during serum deprivation.
Authors: Pestana, Cezar Rangel and Oishi, Jorge Camargo and Salistre-Araújo, Heloísa Sobreiro and Rodrigues, Gerson Jhonatan
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2015): 1168-77

Traumatic brain injury disrupts cerebrovascular tone through endothelial inducible nitric oxide synthase expression and nitric oxide gain of function.
Authors: Villalba, Nuria and Sonkusare, Swapnil K and Longden, Thomas A and Tran, Tram L and Sackheim, Adrian M and Nelson, Mark T and Wellman, George C and Freeman, Kalev
Journal: Journal of the American Heart Association (2014): e001474

Generation and characterization of a spontaneously immortalized endothelial cell line from mice microcirculation.
Authors: Loiola, Rodrigo A and Torres, Tathiany C and Aburaya, Carla M and Landgraf, Maristella A and Landgraf, Richardt G and Bosco Pesquero, João and Fernandes, Liliam
Journal: Experimental cell research (2013): 1102-10

TRPV1-mediated UCP2 upregulation ameliorates hyperglycemia-induced endothelial dysfunction.
Authors: Sun, Jing and Pu, Yunfei and Wang, Peijian and Chen, Sijiao and Zhao, Yu and Liu, Chan and Shang, Qianhui and Zhu, Zhiming and Liu, Daoyan
Journal: Cardiovascular diabetology (2013): 69