DAF-2 DA
DAF-2 diacetate is a more cell-permeable version of DAF-2, a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO). Upon entry into cells, DAF-2 diacetate is hydrolyzed to the less cell-permeable DAF-2 by cellular esterases, thus preventing loss of signal due to leakage of DAF-2 diacetate from cells. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein. The intracellular fluorescence (generated by DAF-2 diacetate) can be readily monitored using the standard FITC filter set. DAF-2 diacetate can be utilized in cells which produce small amounts of NO, such as endothelial cells, as well as in cells which generate large amounts of NO, such as macrophages.
Citations
View all 1 citations: Citation Explorer
Expanded characterization of in vitro polarized M0, M1, and M2 human monocyte-derived macrophages: Bioenergetic and secreted mediator profiles
Authors: Hickman, Elise and Smyth, Timothy and Cobos-Uribe, Catalina and Immormino, Robert and Rebuli, Meghan E and Moran, Timothy and Alexis, Neil E and Jaspers, Ilona
Journal: Plos one (2023): e0279037
Authors: Hickman, Elise and Smyth, Timothy and Cobos-Uribe, Catalina and Immormino, Robert and Rebuli, Meghan E and Moran, Timothy and Alexis, Neil E and Jaspers, Ilona
Journal: Plos one (2023): e0279037
References
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Authors: Zhang, Lin and Zeng, Min and Fu, Bingmei M
Journal: American journal of physiology. Heart and circulatory physiology (2016): H1735-47
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