DAF-2 DA | AAT Bioquest

DAF-2 DA

DAF-2 diacetate is a more cell-permeable version of DAF-2, a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO). Upon entry into cells, DAF-2 diacetate is hydrolyzed to the less cell-permeable DAF-2 by cellular esterases, thus preventing loss of signal due to leakage of DAF-2 diacetate from cells. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein. The intracellular fluorescence (generated by DAF-2 diacetate) can be readily monitored using the standard FITC filter set. DAF-2 diacetate can be utilized in cells which produce small amounts of NO, such as endothelial cells, as well as in cells which generate large amounts of NO, such as macrophages.

Protocol

COA

Catalog	Size	Price	Quantity
16293	10x50 ug	Price
16294	1 mg	Price

Citations

View all 2 citations: Citation Explorer

Water-Driven Oxygen and Nitric Oxide Release from Porphyrin-and Mn-Based Metal--Organic Framework Enables Accelerated Acute Wound Healing

Authors:

Wang, Jieh-Neng and Tu, Zih-Yu and Wang, Wen-Jyun and Li, Wei-Peng and Chen, Wei-Ling and Kan, Chung-Dann

Journal:

ACS Applied Bio Materials (2025)

Expanded characterization of in vitro polarized M0, M1, and M2 human monocyte-derived macrophages: Bioenergetic and secreted mediator profiles

Authors:

Hickman, Elise and Smyth, Timothy and Cobos-Uribe, Catalina and Immormino, Robert and Rebuli, Meghan E and Moran, Timothy and Alexis, Neil E and Jaspers, Ilona

Journal:

Plos one (2023): e0279037

References

View all 50 references: Citation Explorer

Involvement of nitrate reductase-dependent nitric oxide production in magnetopriming-induced salt tolerance in soybean.

Authors:

Kataria, Sunita and Jain, Meeta and Tripathi, Durgesh K and Singh, Vijay P

Journal:

Physiologia plantarum (2020): 422-436

Does Melatonin Exert Its Effect on Ram Sperm Capacitation Through Nitric Oxide Synthase Regulation?

Authors:

Miguel-Jiménez, Sara and Carvajal-Serna, Melissa and Calvo, Silvia and Casao, Adriana and Cebrián-Pérez, José Álvaro and Muiño-Blanco, Teresa and Pérez-Pe, Rosaura

Journal:

International journal of molecular sciences (2020)

Curcumin prevents strokes in stroke-prone spontaneously hypertensive rats by improving vascular endothelial function.

Authors:

Lan, Cong and Chen, Xinjian and Zhang, Yuxun and Wang, Wei and Wang, Wei Eric and Liu, Yukai and Cai, Yue and Ren, Hongmei and Zheng, Shuo and Zhou, Lin and Zeng, Chunyu

Journal:

BMC cardiovascular disorders (2018): 43

Nitric oxide in frozen-thawed equine sperm: Effects on motility, membrane integrity and sperm capacitation.

Authors:

de Andrade, André F C and Arruda, Rubens P and Torres, Mariana A and Pieri, Naira C G and Leite, Ticiano G and Celeghini, Eneiva Carla C and Oliveira, Leticia Z and Gardés, Thayna P and Bussiere, Maria Clara C and Silva, Daniela F

Journal:

Animal reproduction science (2018): 176-184

Inhibition of endothelial nitric oxide synthase decreases breast cancer cell MDA-MB-231 adhesion to intact microvessels under physiological flows.

Authors:

Zhang, Lin and Zeng, Min and Fu, Bingmei M

Journal:

American journal of physiology. Heart and circulatory physiology (2016): H1735-47

Physical properties

Molecular weight	446.42
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	205391-02-2

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Page updated on October 12, 2025