DAF-FM
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 412.35 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Reactive Oxygen Species (ROS)
CAS 254109-20-1 | Molecular weight 412.35 |
DAF-2 is the original compound and is widely used for detecting nitric oxide (NO) in cells. DAF-FM is an improved version of DAF. Compared with DAF-2, DAF-FM is a much more sensitive and photo-stable fluorescent probe for detecting NO. In addition, DAF-FM is much less pH sensitive than DAF-2, essentially pH-independent in the physiological pH range. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DAF-FM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 242.512 µL | 1.213 mL | 2.425 mL | 12.126 mL | 24.251 mL |
5 mM | 48.502 µL | 242.512 µL | 485.025 µL | 2.425 mL | 4.85 mL |
10 mM | 24.251 µL | 121.256 µL | 242.512 µL | 1.213 mL | 2.425 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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References
View all 50 references: Citation Explorer
Nitro-Oleic Acid in Seeds and Differently Developed Seedlings of Brassica napus L.
Authors: Vollár, Martin and Feigl, Gábor and Oláh, Dóra and Horváth, Attila and Molnár, Árpád and Kúsz, Norbert and Ördög, Attila and Csupor, Dezső and Kolbert, Zsuzsanna
Journal: Plants (Basel, Switzerland) (2020)
Authors: Vollár, Martin and Feigl, Gábor and Oláh, Dóra and Horváth, Attila and Molnár, Árpád and Kúsz, Norbert and Ördög, Attila and Csupor, Dezső and Kolbert, Zsuzsanna
Journal: Plants (Basel, Switzerland) (2020)
Omentin-1 protects against high glucose-induced endothelial dysfunction via the AMPK/PPARδ signaling pathway.
Authors: Liu, Fang and Fang, Shaohong and Liu, Xinxin and Li, Ji and Wang, Xuedong and Cui, Jinjin and Chen, Tao and Li, Zhaoying and Yang, Fan and Tian, Jiangtian and Li, Hulun and Yin, Li and Yu, Bo
Journal: Biochemical pharmacology (2020): 113830
Authors: Liu, Fang and Fang, Shaohong and Liu, Xinxin and Li, Ji and Wang, Xuedong and Cui, Jinjin and Chen, Tao and Li, Zhaoying and Yang, Fan and Tian, Jiangtian and Li, Hulun and Yin, Li and Yu, Bo
Journal: Biochemical pharmacology (2020): 113830
Association between expression of AMPK pathway and adiponectin, leptin, and vascular endothelial function in rats with coronary heart disease.
Authors: Li, J-M and Lu, W and Ye, J and Han, Y and Chen, H and Wang, L-S
Journal: European review for medical and pharmacological sciences (2020): 905-914
Authors: Li, J-M and Lu, W and Ye, J and Han, Y and Chen, H and Wang, L-S
Journal: European review for medical and pharmacological sciences (2020): 905-914
Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues.
Authors: Wany, Aakanksha and Pathak, Pradeep Kumar and Gupta, Kapuganti Jagadis
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 15-26
Authors: Wany, Aakanksha and Pathak, Pradeep Kumar and Gupta, Kapuganti Jagadis
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 15-26
Nitric oxide restores peripheral blood mononuclear cell adhesion against hypoxia via NO-cGMP signalling.
Authors: Behera, Jyotirmaya and Nagarajan, Shunmugam and Saran, Uttara and Kumar, Ravi and Keshri, Gaurav K and Suryakumar, Geetha and Chatterjee, Suvro
Journal: Cell biochemistry and function (2020)
Authors: Behera, Jyotirmaya and Nagarajan, Shunmugam and Saran, Uttara and Kumar, Ravi and Keshri, Gaurav K and Suryakumar, Geetha and Chatterjee, Suvro
Journal: Cell biochemistry and function (2020)
Histamine induces intracellular Ca2+ oscillations and nitric oxide release in endothelial cells from brain microvascular circulation.
Authors: Berra-Romani, Roberto and Faris, Pawan and Pellavio, Giorgia and Orgiu, Matteo and Negri, Sharon and Forcaia, Greta and Var-Gaz-Guadarrama, Verónica and Garcia-Carrasco, Mario and Botta, Laura and Sancini, Giulio and Laforenza, Umberto and Moccia, Francesco
Journal: Journal of cellular physiology (2020): 1515-1530
Authors: Berra-Romani, Roberto and Faris, Pawan and Pellavio, Giorgia and Orgiu, Matteo and Negri, Sharon and Forcaia, Greta and Var-Gaz-Guadarrama, Verónica and Garcia-Carrasco, Mario and Botta, Laura and Sancini, Giulio and Laforenza, Umberto and Moccia, Francesco
Journal: Journal of cellular physiology (2020): 1515-1530
Stretch-activated Piezo1 Channel in Endothelial Cells Relaxes Mouse Intrapulmonary Arteries.
Authors: Lhomme, Audrey and Gilbert, Guillaume and Pele, Thomas and Deweirdt, Juliette and Henrion, Daniel and Baudrimont, Isabelle and Campagnac, Marilyne and Marthan, Roger and Guibert, Christelle and Ducret, Thomas and Savineau, Jean-Pierre and Quignard, Jean-François
Journal: American journal of respiratory cell and molecular biology (2019): 650-658
Authors: Lhomme, Audrey and Gilbert, Guillaume and Pele, Thomas and Deweirdt, Juliette and Henrion, Daniel and Baudrimont, Isabelle and Campagnac, Marilyne and Marthan, Roger and Guibert, Christelle and Ducret, Thomas and Savineau, Jean-Pierre and Quignard, Jean-François
Journal: American journal of respiratory cell and molecular biology (2019): 650-658
7-Keto-Cholesterol and Cholestan-3beta, 5alpha, 6beta-Triol Induce Eryptosis through Distinct Pathways Leading to NADPH Oxidase and Nitric Oxide Synthase Activation.
Authors: Attanzio, Alessandro and Frazzitta, Anna and Cilla, Antonio and Livrea, Maria A and Tesoriere, Luisa and Allegra, Mario
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2019): 933-947
Authors: Attanzio, Alessandro and Frazzitta, Anna and Cilla, Antonio and Livrea, Maria A and Tesoriere, Luisa and Allegra, Mario
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2019): 933-947
Differential radio-adaptive responses in BALB/c and C57BL/6 mice: pivotal role of calcium and nitric oxide signalling.
Authors: Premkumar, Kavitha and Nair, Jisha and Shankar, Bhavani S
Journal: International journal of radiation biology (2019): 655-666
Authors: Premkumar, Kavitha and Nair, Jisha and Shankar, Bhavani S
Journal: International journal of radiation biology (2019): 655-666
Selective inhibition of histone deacetylase 8 improves vascular hypertrophy, relaxation, and inflammation in angiotensin II hypertensive mice.
Authors: Kee, Hae Jin and Ryu, Yuhee and Seok, Young Mi and Choi, Sin Young and Sun, Simei and Kim, Gwi Ran and Jeong, Myung Ho
Journal: Clinical hypertension (2019): 13
Authors: Kee, Hae Jin and Ryu, Yuhee and Seok, Young Mi and Choi, Sin Young and Sun, Simei and Kim, Gwi Ran and Jeong, Myung Ho
Journal: Clinical hypertension (2019): 13
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Dopamine-Mediated Oxidation of Methionine 127 in α-Synuclein
Hydrogen peroxide detection with high specificity in living cells and inflamed tissues
L-BMAA Induced ER Stress and Enhanced Caspase 12 Cleavage at Low Nonexcitotoxic Concentrations
Interactome analysis reveals a n 1 ovel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage
Dopamine-Mediated Oxidation of Methionine 127 in α-Synuclein
Hydrogen peroxide detection with high specificity in living cells and inflamed tissues
L-BMAA Induced ER Stress and Enhanced Caspase 12 Cleavage at Low Nonexcitotoxic Concentrations
Interactome analysis reveals a n 1 ovel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage