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DAF-FM

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Physical properties
Molecular weight412.35
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
254109-20-1
Molecular weight
412.35
DAF-2 is the original compound and is widely used for detecting nitric oxide (NO) in cells. DAF-FM is an improved version of DAF. Compared with DAF-2, DAF-FM is a much more sensitive and photo-stable fluorescent probe for detecting NO. In addition, DAF-FM is much less pH sensitive than DAF-2, essentially pH-independent in the physiological pH range. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DAF-FM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM242.512 µL1.213 mL2.425 mL12.126 mL24.251 mL
5 mM48.502 µL242.512 µL485.025 µL2.425 mL4.85 mL
10 mM24.251 µL121.256 µL242.512 µL1.213 mL2.425 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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References


View all 50 references: Citation Explorer
Nitro-Oleic Acid in Seeds and Differently Developed Seedlings of Brassica napus L.
Authors: Vollár, Martin and Feigl, Gábor and Oláh, Dóra and Horváth, Attila and Molnár, Árpád and Kúsz, Norbert and Ördög, Attila and Csupor, Dezső and Kolbert, Zsuzsanna
Journal: Plants (Basel, Switzerland) (2020)
Omentin-1 protects against high glucose-induced endothelial dysfunction via the AMPK/PPARδ signaling pathway.
Authors: Liu, Fang and Fang, Shaohong and Liu, Xinxin and Li, Ji and Wang, Xuedong and Cui, Jinjin and Chen, Tao and Li, Zhaoying and Yang, Fan and Tian, Jiangtian and Li, Hulun and Yin, Li and Yu, Bo
Journal: Biochemical pharmacology (2020): 113830
Association between expression of AMPK pathway and adiponectin, leptin, and vascular endothelial function in rats with coronary heart disease.
Authors: Li, J-M and Lu, W and Ye, J and Han, Y and Chen, H and Wang, L-S
Journal: European review for medical and pharmacological sciences (2020): 905-914
Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues.
Authors: Wany, Aakanksha and Pathak, Pradeep Kumar and Gupta, Kapuganti Jagadis
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 15-26
Nitric oxide restores peripheral blood mononuclear cell adhesion against hypoxia via NO-cGMP signalling.
Authors: Behera, Jyotirmaya and Nagarajan, Shunmugam and Saran, Uttara and Kumar, Ravi and Keshri, Gaurav K and Suryakumar, Geetha and Chatterjee, Suvro
Journal: Cell biochemistry and function (2020)
Histamine induces intracellular Ca2+ oscillations and nitric oxide release in endothelial cells from brain microvascular circulation.
Authors: Berra-Romani, Roberto and Faris, Pawan and Pellavio, Giorgia and Orgiu, Matteo and Negri, Sharon and Forcaia, Greta and Var-Gaz-Guadarrama, Verónica and Garcia-Carrasco, Mario and Botta, Laura and Sancini, Giulio and Laforenza, Umberto and Moccia, Francesco
Journal: Journal of cellular physiology (2020): 1515-1530
L-sepiapterin restores SLE serum-induced markers of endothelial function in endothelial cells.
Authors: Jones Buie, Joy N and Pleasant Jenkins, Dorea and Muise-Helmericks, Robin and Oates, Jim C
Journal: Lupus science & medicine (2019): e000294
Design, synthesis and biological study of hybrid drug candidates of nitric oxide releasing cucurbitacin-inspired estrone analogs for treatment of hepatocellular carcinoma.
Authors: Abou-Salim, Mahrous A and Shaaban, Mohamed A and Abd El Hameid, Mohammed K and Elshaier, Yaseen A M M and Halaweish, Fathi
Journal: Bioorganic chemistry (2019): 515-533
Oxysterol modulates neurotransmission via liver-X receptor/NO synthase-dependent pathway at the mouse neuromuscular junctions.
Authors: Mukhutdinova, Kamilla A and Kasimov, Marat R and Zakyrjanova, Guzel F and Gumerova, Milausha R and Petrov, Alexey M
Journal: Neuropharmacology (2019): 70-79
Androgens drive microvascular endothelial dysfunction in women with polycystic ovary syndrome: role of the endothelin B receptor.
Authors: Usselman, Charlotte W and Yarovinsky, Timur O and Steele, Frances E and Leone, Cheryl A and Taylor, Hugh S and Bender, Jeffrey R and Stachenfeld, Nina S
Journal: The Journal of physiology (2019): 2853-2865