logo
AAT Bioquest

DAF-FM

Product Image
Product Image
Gallery Image 1
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight412.35
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
254109-20-1
Molecular weight
412.35
DAF-2 is the original compound and is widely used for detecting nitric oxide (NO) in cells. DAF-FM is an improved version of DAF. Compared with DAF-2, DAF-FM is a much more sensitive and photo-stable fluorescent probe for detecting NO. In addition, DAF-FM is much less pH sensitive than DAF-2, essentially pH-independent in the physiological pH range. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DAF-FM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM242.512 µL1.213 mL2.425 mL12.126 mL24.251 mL
5 mM48.502 µL242.512 µL485.025 µL2.425 mL4.85 mL
10 mM24.251 µL121.256 µL242.512 µL1.213 mL2.425 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Images


References


View all 50 references: Citation Explorer
Nitro-Oleic Acid in Seeds and Differently Developed Seedlings of Brassica napus L.
Authors: Vollár, Martin and Feigl, Gábor and Oláh, Dóra and Horváth, Attila and Molnár, Árpád and Kúsz, Norbert and Ördög, Attila and Csupor, Dezső and Kolbert, Zsuzsanna
Journal: Plants (Basel, Switzerland) (2020)
Omentin-1 protects against high glucose-induced endothelial dysfunction via the AMPK/PPARδ signaling pathway.
Authors: Liu, Fang and Fang, Shaohong and Liu, Xinxin and Li, Ji and Wang, Xuedong and Cui, Jinjin and Chen, Tao and Li, Zhaoying and Yang, Fan and Tian, Jiangtian and Li, Hulun and Yin, Li and Yu, Bo
Journal: Biochemical pharmacology (2020): 113830
Association between expression of AMPK pathway and adiponectin, leptin, and vascular endothelial function in rats with coronary heart disease.
Authors: Li, J-M and Lu, W and Ye, J and Han, Y and Chen, H and Wang, L-S
Journal: European review for medical and pharmacological sciences (2020): 905-914
Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues.
Authors: Wany, Aakanksha and Pathak, Pradeep Kumar and Gupta, Kapuganti Jagadis
Journal: Methods in molecular biology (Clifton, N.J.) (2020): 15-26
Nitric oxide restores peripheral blood mononuclear cell adhesion against hypoxia via NO-cGMP signalling.
Authors: Behera, Jyotirmaya and Nagarajan, Shunmugam and Saran, Uttara and Kumar, Ravi and Keshri, Gaurav K and Suryakumar, Geetha and Chatterjee, Suvro
Journal: Cell biochemistry and function (2020)
Histamine induces intracellular Ca2+ oscillations and nitric oxide release in endothelial cells from brain microvascular circulation.
Authors: Berra-Romani, Roberto and Faris, Pawan and Pellavio, Giorgia and Orgiu, Matteo and Negri, Sharon and Forcaia, Greta and Var-Gaz-Guadarrama, Verónica and Garcia-Carrasco, Mario and Botta, Laura and Sancini, Giulio and Laforenza, Umberto and Moccia, Francesco
Journal: Journal of cellular physiology (2020): 1515-1530
Stretch-activated Piezo1 Channel in Endothelial Cells Relaxes Mouse Intrapulmonary Arteries.
Authors: Lhomme, Audrey and Gilbert, Guillaume and Pele, Thomas and Deweirdt, Juliette and Henrion, Daniel and Baudrimont, Isabelle and Campagnac, Marilyne and Marthan, Roger and Guibert, Christelle and Ducret, Thomas and Savineau, Jean-Pierre and Quignard, Jean-François
Journal: American journal of respiratory cell and molecular biology (2019): 650-658
7-Keto-Cholesterol and Cholestan-3beta, 5alpha, 6beta-Triol Induce Eryptosis through Distinct Pathways Leading to NADPH Oxidase and Nitric Oxide Synthase Activation.
Authors: Attanzio, Alessandro and Frazzitta, Anna and Cilla, Antonio and Livrea, Maria A and Tesoriere, Luisa and Allegra, Mario
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2019): 933-947
Differential radio-adaptive responses in BALB/c and C57BL/6 mice: pivotal role of calcium and nitric oxide signalling.
Authors: Premkumar, Kavitha and Nair, Jisha and Shankar, Bhavani S
Journal: International journal of radiation biology (2019): 655-666
Selective inhibition of histone deacetylase 8 improves vascular hypertrophy, relaxation, and inflammation in angiotensin II hypertensive mice.
Authors: Kee, Hae Jin and Ryu, Yuhee and Seok, Young Mi and Choi, Sin Young and Sun, Simei and Kim, Gwi Ran and Jeong, Myung Ho
Journal: Clinical hypertension (2019): 13