AAT Bioquest


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Physical properties
Molecular weight412.35
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
DAF-2 is the original compound and is widely used for detecting nitric oxide (NO) in cells. DAF-FM is an improved version of DAF. Compared with DAF-2, DAF-FM is a much more sensitive and photo-stable fluorescent probe for detecting NO. In addition, DAF-FM is much less pH sensitive than DAF-2, essentially pH-independent in the physiological pH range. DAF-FM is not quite as cell-permeable as the diacetate derivative, DAF-FM, but it does not need to be activated by cytosolic enzymes.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DAF-FM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM242.512 µL1.213 mL2.425 mL12.126 mL24.251 mL
5 mM48.502 µL242.512 µL485.025 µL2.425 mL4.85 mL
10 mM24.251 µL121.256 µL242.512 µL1.213 mL2.425 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles



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