DAF-FM diacetate
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 496.42 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Reactive Oxygen Species (ROS)
CAS 254109-22-3 | Molecular weight 496.42 |
DAF-FM diacetate is a more cell-permeable version of DAF-FM, a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO). Upon entry into cells, DAF-FM diacetate is hydrolyzed to the less cell-permeable DAF-FM by cellular esterases, thus preventing loss of signal due to leakage of DAF-FM diacetate from cells. In the presence of oxygen, DAF-FM reacts with NO to yield the highly fluorescent difluoro-triazolofluorescein. The intracellular fluorescence (generated by DAF-FM diacetate) can be readily monitored using the standard FITC filter set. DAF-FM diacetate can be utilized in cells for a broad range of NO production, e.g., the small amounts of NO production (such as in endothelial cells) and large amounts of NO production (such as in macrophages). Compared with DAF-2 DA, DAF-FM diacetate is a much more sensitive and photo-stable fluorescent probe for detecting NO. In addition, DAF-FM is much less pH sensitive than DAF-2, essentially pH-independent in the physiological pH range.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DAF-FM diacetate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 201.442 µL | 1.007 mL | 2.014 mL | 10.072 mL | 20.144 mL |
5 mM | 40.288 µL | 201.442 µL | 402.885 µL | 2.014 mL | 4.029 mL |
10 mM | 20.144 µL | 100.721 µL | 201.442 µL | 1.007 mL | 2.014 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
References
View all 2 references: Citation Explorer
A reliable fluorimetric method to screen the nitric oxide synthase inhibitors in 96 well plate.
Authors: Agrawal, Saurabh and Kumari, Rita and Luthra, Pratibha Mehta
Journal: Analytical biochemistry (2019): 42-44
Authors: Agrawal, Saurabh and Kumari, Rita and Luthra, Pratibha Mehta
Journal: Analytical biochemistry (2019): 42-44
Modification of endoplasmic reticulum Ca2+ stores by select oxidants produces changes reminiscent of those in cells from patients with Alzheimer disease.
Authors: Huang, Hsueh-Meei and Chen, Huan-Lian and Xu, Hui and Gibson, Gary E
Journal: Free radical biology & medicine (2005): 979-89
Authors: Huang, Hsueh-Meei and Chen, Huan-Lian and Xu, Hui and Gibson, Gary E
Journal: Free radical biology & medicine (2005): 979-89
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells