AAT Bioquest

DAF-FM diacetate

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Physical properties
Molecular weight496.42
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
DAF-FM diacetate is a more cell-permeable version of DAF-FM, a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO). Upon entry into cells, DAF-FM diacetate is hydrolyzed to the less cell-permeable DAF-FM by cellular esterases, thus preventing loss of signal due to leakage of DAF-FM diacetate from cells. In the presence of oxygen, DAF-FM reacts with NO to yield the highly fluorescent difluoro-triazolofluorescein. The intracellular fluorescence (generated by DAF-FM diacetate) can be readily monitored using the standard FITC filter set. DAF-FM diacetate can be utilized in cells for a broad range of NO production, e.g., the small amounts of NO production (such as in endothelial cells) and large amounts of NO production (such as in macrophages). Compared with DAF-2 DA, DAF-FM diacetate is a much more sensitive and photo-stable fluorescent probe for detecting NO. In addition, DAF-FM is much less pH sensitive than DAF-2, essentially pH-independent in the physiological pH range.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DAF-FM diacetate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM201.442 µL1.007 mL2.014 mL10.072 mL20.144 mL
5 mM40.288 µL201.442 µL402.885 µL2.014 mL4.029 mL
10 mM20.144 µL100.721 µL201.442 µL1.007 mL2.014 mL

Molarity calculator

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View all 2 references: Citation Explorer
A reliable fluorimetric method to screen the nitric oxide synthase inhibitors in 96 well plate.
Authors: Agrawal, Saurabh and Kumari, Rita and Luthra, Pratibha Mehta
Journal: Analytical biochemistry (2019): 42-44
Modification of endoplasmic reticulum Ca2+ stores by select oxidants produces changes reminiscent of those in cells from patients with Alzheimer disease.
Authors: Huang, Hsueh-Meei and Chen, Huan-Lian and Xu, Hui and Gibson, Gary E
Journal: Free radical biology & medicine (2005): 979-89