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DAF-FM diacetate
DAF-FM diacetate is a more cell-permeable version of DAF-FM, a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO). Upon entry into cells, DAF-FM diacetate is hydrolyzed to the less cell-permeable DAF-FM by cellular esterases, thus preventing loss of signal due to leakage of DAF-FM diacetate from cells. In the presence of oxygen, DAF-FM reacts with NO to yield the highly fluorescent difluoro-triazolofluorescein. The intracellular fluorescence (generated by DAF-FM diacetate) can be readily monitored using the standard FITC filter set. DAF-FM diacetate can be utilized in cells for a broad range of NO production, e.g., the small amounts of NO production (such as in endothelial cells) and large amounts of NO production (such as in macrophages). Compared with DAF-2 DA, DAF-FM diacetate is a much more sensitive and photo-stable fluorescent probe for detecting NO. In addition, DAF-FM is much less pH sensitive than DAF-2, essentially pH-independent in the physiological pH range.
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 16298 | 10x50 ug | Price | |
| 16299 | 1 mg | Price |
Physical properties
| Molecular weight | 496.42 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| CAS | 254109-22-3 |
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| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
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| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 22, 2025