Dansyl cadaverine [5-Dimethylaminonaphthalene-1-(N-(5-aminopentyl))sulfonamide]

Dansyl cadaverine

5-Dimethylaminonaphthalene-1-(N-(5-aminopentyl))sulfonamide

Dansyl cadaverine is an amine-containing Dansyl building block. Dansyl cadaverine is also the most popular fluorescent transglutaminase substrate to label proteins by transaminidation. It has also been used to prepare other small fluorescent biomolecules via amidation or reductive amination. It should be noted that the Dansyl fluorescence is highly environment-sensitive. Dansyl dyes have environmentally sensitive fluorescence quantum yields and emission maxima along with large Stokes shifts. This environment-sensitive fluorescence property has made Dansyl dyes an important tool for biophysical studies. They are particularly useful for preparing fluorescent drug or ligand analogs that are expected to bind to hydrophobic sites in proteins or membranes or other biological receptors. Dansyl protein conjugates have fluorescence lifetimes of 10-20 nanoseconds.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
810	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Dansyl cadaverine [5-Dimethylaminonaphthalene-1-(N-(5-aminopentyl))sulfonamide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	298.098 µL	1.49 mL	2.981 mL	14.905 mL	29.81 mL
5 mM	59.62 µL	298.098 µL	596.196 µL	2.981 mL	5.962 mL
10 mM	29.81 µL	149.049 µL	298.098 µL	1.49 mL	2.981 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	335.46 g/mol	=	mL	x	mM	=	mmol

References

View all 33 references: Citation Explorer

Phenylisothiocyanate and dansyl chloride precolumn derivatizations for the high-performance liquid chromatography analysis of the antitumoral agent ES-285 in dog plasma

Authors:

Maraschiello C, Mir and a E, Millan E, Floriano P, Vilageliu J.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2003): 1

Dansyl chloride derivatization of methamphetamine: a method with advantages for screening and analysis of methamphetamine in urine

Authors:

Yamada H, Yamahara A, Yasuda S, Abe M, Oguri K, Fukushima S, Ikeda-Wada S.

Journal:

J Anal Toxicol (2002): 17

Determination of the xenoestrogens 4-nonylphenol and bisphenol A by high-performance liquid chromatography and fluorescence detection after derivatisation with dansyl chloride

Authors:

Naassner M, Mergler M, Wolf K, Schuphan I.

Journal:

J Chromatogr A (2002): 133

Automated pre-column derivatization of amines in biological samples with dansyl chloride and with or without post-column chemiluminescence formation by using TCPO-H2O2

Authors:

Molins-Legua C, Campins-Falco P, Sevillano-Cabeza A.

Journal:

Analyst (1998): 2871

A new screening method for amphetamine and methamphetamine using dansyl chloride derivatization and cartridge fluorescence

Authors:

Yamada H, Ikeda-Wada S, Oguri K.

Journal:

Biol Pharm Bull (1998): 778

Physical properties

Molecular weight	335.46
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.387
Extinction coefficient (cm ^-1 M ^-1)	3300
Excitation (nm)	335
Emission (nm)	518

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	10121-91-2

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Page updated on October 12, 2025