Dansyl chloride [5-Dimethylaminonaphthalene-1-sulfonyl chloride] *CAS 605-65-2*

Dansyl chloride

5-Dimethylaminonaphthalene-1-sulfonyl chloride; CAS 605-65-2

Dansyl chloride is non-fluorescent until it reacts with amines. The resulting Dansyl amides have environmentally sensitive fluorescence quantum yields and emission maxima along with large Stokes shifts. This environment-sensitive fluorescence property has made Dansyl chloride an important tool for biophysical studies. It is particularly useful for preparing fluorescent drug or ligand analogs that are expected to bind to hydrophobic sites in proteins or membranes or other biological receptors. Dansyl protein conjugates have fluorescence lifetimes of 10-20 nanoseconds.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
811	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of Dansyl chloride [5-Dimethylaminonaphthalene-1-sulfonyl chloride] *CAS 605-65-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	370.714 µL	1.854 mL	3.707 mL	18.536 mL	37.071 mL
5 mM	74.143 µL	370.714 µL	741.427 µL	3.707 mL	7.414 mL
10 mM	37.071 µL	185.357 µL	370.714 µL	1.854 mL	3.707 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	269.75 g/mol	=	mL	x	mM	=	mmol

References

View all 33 references: Citation Explorer

Phenylisothiocyanate and dansyl chloride precolumn derivatizations for the high-performance liquid chromatography analysis of the antitumoral agent ES-285 in dog plasma

Authors:

Maraschiello C, Mir and a E, Millan E, Floriano P, Vilageliu J.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2003): 1

Dansyl chloride derivatization of methamphetamine: a method with advantages for screening and analysis of methamphetamine in urine

Authors:

Yamada H, Yamahara A, Yasuda S, Abe M, Oguri K, Fukushima S, Ikeda-Wada S.

Journal:

J Anal Toxicol (2002): 17

Determination of the xenoestrogens 4-nonylphenol and bisphenol A by high-performance liquid chromatography and fluorescence detection after derivatisation with dansyl chloride

Authors:

Naassner M, Mergler M, Wolf K, Schuphan I.

Journal:

J Chromatogr A (2002): 133

Automated pre-column derivatization of amines in biological samples with dansyl chloride and with or without post-column chemiluminescence formation by using TCPO-H2O2

Authors:

Molins-Legua C, Campins-Falco P, Sevillano-Cabeza A.

Journal:

Analyst (1998): 2871

A new screening method for amphetamine and methamphetamine using dansyl chloride derivatization and cartridge fluorescence

Authors:

Yamada H, Ikeda-Wada S, Oguri K.

Journal:

Biol Pharm Bull (1998): 778

Physical properties

Molecular weight	269.75
Solvent	DMF

Spectral properties

Correction factor (280 nm)	0.387
Extinction coefficient (cm ^-1 M ^-1)	3300
Excitation (nm)	335
Emission (nm)	518

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	605-65-2

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 13, 2025