Dansyl-X, acid

Compared to Dansyl chloride, Dansyl-X acid gives better yield for labeling peptides and nucleotides. Dansyl dyes have environmentally sensitive fluorescence quantum yields and emission maxima along with large Stokes shifts. This environment-sensitive fluorescence property has made Dansyl dyes an important tool for biophysical studies. They are particularly useful for preparing fluorescent drug or ligand analogs that are expected to bind to hydrophobic sites in proteins or membranes or other biological receptors. Dansyl protein conjugates have fluorescence lifetimes of 10-20 nanoseconds.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
812	5 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Dansyl-X, acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	274.379 µL	1.372 mL	2.744 mL	13.719 mL	27.438 mL
5 mM	54.876 µL	274.379 µL	548.757 µL	2.744 mL	5.488 mL
10 mM	27.438 µL	137.189 µL	274.379 µL	1.372 mL	2.744 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	364.46 g/mol	=	mL	x	mM	=	mmol

References

View all 33 references: Citation Explorer

Phenylisothiocyanate and dansyl chloride precolumn derivatizations for the high-performance liquid chromatography analysis of the antitumoral agent ES-285 in dog plasma

Authors:

Maraschiello C, Mir and a E, Millan E, Floriano P, Vilageliu J.

Journal:

J Chromatogr B Analyt Technol Biomed Life Sci (2003): 1

Dansyl chloride derivatization of methamphetamine: a method with advantages for screening and analysis of methamphetamine in urine

Authors:

Yamada H, Yamahara A, Yasuda S, Abe M, Oguri K, Fukushima S, Ikeda-Wada S.

Journal:

J Anal Toxicol (2002): 17

Determination of the xenoestrogens 4-nonylphenol and bisphenol A by high-performance liquid chromatography and fluorescence detection after derivatisation with dansyl chloride

Authors:

Naassner M, Mergler M, Wolf K, Schuphan I.

Journal:

J Chromatogr A (2002): 133

Automated pre-column derivatization of amines in biological samples with dansyl chloride and with or without post-column chemiluminescence formation by using TCPO-H2O2

Authors:

Molins-Legua C, Campins-Falco P, Sevillano-Cabeza A.

Journal:

Analyst (1998): 2871

A new screening method for amphetamine and methamphetamine using dansyl chloride derivatization and cartridge fluorescence

Authors:

Yamada H, Ikeda-Wada S, Oguri K.

Journal:

Biol Pharm Bull (1998): 778

Physical properties

Molecular weight	364.46
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.387
Extinction coefficient (cm ^-1 M ^-1)	3300
Excitation (nm)	335
Emission (nm)	518

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on April 6, 2022