DAPI | AAT Bioquest

DAPI

4,6-Diamidino-2-phenylindole, dihydrochloride; 10 mM solution in water

DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 500 nm when bound to RNA. DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures.

Fluorescence image of lung tissue stained for EpCAM. Lung tissue was incubated with an EpCAM primary antibody followed by a purified GXM HRP-conjugated secondary antibody. Signal was developed using a mixture of XFD 532 TSA (Cat. #11092) and iFluor® 532 PSA (Cat. #45023). Nuclei were counterstained with DAPI, and images were acquired using TRITC and DAPI filter sets.

Datasheet

SDS

Catalog	Size	Price	Quantity
17507	2 mL	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *10 mM solution in water* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	285.51 µL	1.428 mL	2.855 mL	14.276 mL	28.551 mL
5 mM	57.102 µL	285.51 µL	571.021 µL	2.855 mL	5.71 mL
10 mM	28.551 µL	142.755 µL	285.51 µL	1.428 mL	2.855 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	350.25 g/mol	=	mL	x	mM	=	mmol

Citations

View all 55 citations: Citation Explorer

Adipose tissue explant culture using PDMS flow chambers: an alternative to static explant culture

Authors:

Cohen, M and Bandaru, P and Szylo, K and Nguyen, N and Nadeak, B and Paszkiewicz, R and Ashby, JW and Mack, JJ and Tanaka, L and Tan, J and others,

Journal:

Adipocyte (2025): 2578286

Inhibitory impact of a mesoporous silica nanoparticle-based drug delivery system on Porphyromonas gingivalis-induced bone resorption

Authors:

Li, Mengya and Sun, Jian and Zhao, Dong and Zhang, Wen and Xu, Qingan

Journal:

Journal of Materials Science: Materials in Medicine (2024): 56

Temperature switchable linkers suitable for triggered drug release in cancer thermo-chemotherapy

Authors:

Ye, Junxiao and Cui, Hui and Liu, Ergang and Pei, Xing and Chai, Meihong and Sun, Lu and Wang, Dongmei and Yang, Victor C and Yu, Fei

Journal:

International Journal of Pharmaceutics (2024): 124757

Tristetraprolin affects invasion-associated genes expression and cell motility in triple-negative breast cancer model

Authors:

Hubiernatorova, Anastasiia and Novak, Josef and Vaskovicova, Michaela and Sekac, David and Kropyvko, Serhii and Hodny, Zdenek

Journal:

Cytoskeleton (2024)

An NIR-responsive hydrogel loaded with polydeoxyribonucleotide nano-vectors for enhanced chronic wound healing

Authors:

Sun, Yanzhen and Li, Yao and Ding, Xiaokang and Xu, Pan and Jing, Xiaodong and Cong, Hailin and Hu, Hao and Yu, Bing and Xu, Fu-Jian

Journal:

Biomaterials (2024): 122789

References

View all 134 references: Citation Explorer

Physical localization of ribosomal genes and chromosome DAPI banding by in situ hybridization in Medicago sativa L

Authors:

Chen JM, Hong YH, Wang YP, Bowley S, Wan JM.

Journal:

Yi Chuan (2006): 184

DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I

Authors:

Gichner T, Mukherjee A, Veleminsky J.

Journal:

Mutat Res (2006): 17

Identification of all pachytene bivalents in the common shrew using DAPI-staining of synaptonemal complex spreads

Authors:

Belonogova NM, Karamysheva TV, Biltueva LS, Perepelov EA, Minina JM, Polyakov AV, Zhdanova NS, Rubtsov NB, Searle JB, Borodin PM.

Journal:

Chromosome Res (2006): 673

Reliable DNA ploidy determination in dehydrated tissues of vascular plants by DAPI flow cytometry--new prospects for plant research

Authors:

Suda J, Travnicek P.

Journal:

Cytometry A (2006): 273

Investigation of chromosomes in varieties and translocation lines of pea Pisum sativum L. by FISH, Ag-NOR, and differential DAPI staining

Authors:

Samatadze TE, Muravenko OM, Bol'sheva NL, Amosova AB, Gostimsckii SA, Zelenin AV.

Journal:

Genetika (2005): 1665

Physical properties

Molecular weight	350.25
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	359
Emission (nm)	457

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	28718-90-3

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Page updated on September 19, 2024