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DASPEI [2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide] *CAS#: 3785-01-1*

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Physical properties
Molecular weight380.27
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


See also: Mitochondria
CAS
3785-01-1
Molecular weight
380.27
DASPEI is a styryl dye that stains mitochondria of live cells. This dye has a large fluorescence Stokes shift and is taken up relatively slowly as a function of membrane. The vital mitochondrial dye DASPEI has been used for the rapid visualization of several distinct classes of epidermal cells in vivo and in vitro: epidermal electroreceptors, mechanoreceptors, and chloride cells in teleosts, and mechanoreceptors in amphibians. DASPEI is also used to locate a different type of sensory cells. In vivo, bath-applied DASPEI stains entire larvae in a relatively non-specific fashion.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DASPEI [2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide] *CAS#: 3785-01-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM262.971 µL1.315 mL2.63 mL13.149 mL26.297 mL
5 mM52.594 µL262.971 µL525.942 µL2.63 mL5.259 mL
10 mM26.297 µL131.486 µL262.971 µL1.315 mL2.63 mL

Molarity calculator

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Citations


View all 1 citations: Citation Explorer
Environmental Hazard Potential of Nano-Photocatalysts Determined by Nano-Bio Interactions and Exposure Conditions
Authors: He, Yuan and Peng, Guotao and Jiang, Yue and Zhao, Mei and Wang, Xiaoxiao and Chen, Mengmeng and Lin, Sijie
Journal: Small (2020): 1907690

References


View all 17 references: Citation Explorer
Development of a liquid chromatography/mass spectrometry-based drug accumulation assay in Pseudomonas aeruginosa
Authors: Cai H, Rose K, Liang LH, Dunham S, Stover C.
Journal: Anal Biochem (2009): 321
Epithelial mitochondria-rich cells and associated innervation in adult and developing zebrafish
Authors: Jonz MG, Nurse CA.
Journal: J Comp Neurol (2006): 817
The use of zebrafish for assessing ototoxic and otoprotective agents
Authors: Ton C, Parng C.
Journal: Hear Res (2005): 79
Mitochondria-rich cell activity in the yolk-sac membrane of tilapia (Oreochromis mossambicus) larvae acclimatized to different ambient chloride levels
Authors: Lin LY, Hwang PP.
Journal: J Exp Biol (2004): 1335
Extramembrane central pore of multidrug exporter AcrB in Escherichia coli plays an important role in drug transport
Authors: Murakami S, Tamura N, Saito A, Hirata T, Yamaguchi A.
Journal: J Biol Chem (2004): 3743
Neomycin-induced hair cell death and rapid regeneration in the lateral line of zebrafish (Danio rerio)
Authors: Harris JA, Cheng AG, Cunningham LL, MacDonald G, Raible DW, Rubel EW.
Journal: J Assoc Res Otolaryngol (2003): 219
Developmental differences in susceptibility to neomycin-induced hair cell death in the lateral line neuromasts of zebrafish (Danio rerio)
Authors: Murakami SL, Cunningham LL, Werner LA, Bauer E, Pujol R, Raible DW, Rubel EW.
Journal: Hear Res (2003): 47
Calcium ion triggers rapid morphological oscillation of chloride cells in the mudskipper, Periophthalmus modestus
Authors: Sakamoto T, Ando M.
Journal: J Comp Physiol B (2002): 435
Mitochondria-rich cells in gills and skin of an African lungfish, Protopterus annectens
Authors: Sturla M, Masini MA, Prato P, Grattarola C, Uva B.
Journal: Cell Tissue Res (2001): 351
Rapid morphological oscillation of mitochondrion-rich cell in estuarine mudskipper following salinity changes
Authors: Sakamoto T, Yokota S, Ando M.
Journal: J Exp Zool (2000): 666